Screening of Dengue Virus Antiviral Activity of Marine Seaweeds by an In Situ Enzyme-Linked Immunosorbent Assay
Publication Date
December 05, 2012
Journal
PLOS ONE
Authors
Andrea Cristine Koishi, Paula Rodrigues Zanello, Éverson Miguel Bianco, Juliano Bordignon, et al
Volume
7
Issue
12
Pages
e51089
DOI
https://dx.plos.org/10.1371/journal.pone.0051089
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0051089
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/23227238
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515490
Europe PMC
http://europepmc.org/abstract/MED/23227238
Web of Science
000312588200098
Scopus
84870798620
Mendeley
http://www.mendeley.com/research/screening-dengue-virus-antiviral-activity-marine-seaweeds-situ-enzymelinked-immunosorbent-assay
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/531107"], "description"=>"<p><i>In situ</i> ELISA assay workflow.</p>", "links"=>[], "tags"=>["elisa", "assay"], "article_id"=>201600, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_situ_ELISA_assay_workflow_/201600", "title"=>"<i>In situ</i> ELISA assay workflow.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:26:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/531187"], "description"=>"<p>(<b>A</b>) Effect of different blocking buffers (skim milk and FBS) and fixatives (methanol:acetone and paraformaldehyde followed by triton X-100) for the assay. (<b>B</b>) Cells were seeded in a range of concentration and the influence for the assay performance was evaluated. (<b>C</b>) The ideal MOI for all DENV serotypes was evaluated 48 hours post-infection and (<b>D</b>) 72 hours post-infection. Values represent mean ± SD of three independent experiments performed in triplicate.</p>", "links"=>[], "tags"=>["elisa"], "article_id"=>201687, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_situ_ELISA_optimization_/201687", "title"=>"<i>In situ</i> ELISA optimization.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:28:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/531295"], "description"=>"<p>(<b>A</b>) Correlation between the <i>in situ</i> ELISA and the foci-forming assay, where Huh7.5 was infected with DENV-1, DENV-2, DENV-3 and DENV-4 at a range of MOI (4–0.01). After 72 hours of infection the supernatant was used for the foci-forming assay and the cells submitted to the ELISA assay. Data from one representative experiment, analyzed using Pearson correlation test. (<b>B</b>) Comparison of the DRC and the IC50 for IFN-α 2A and DENV-4 infection, obtained with the <i>in situ</i> ELISA, foci-forming assay and a commercial NS1 antigen capture ELISA assay. Mean ± SD of three independent experiments, analyzed by sigmoidal dose-response curve (variable slope), the dashed line represents the detection limit of the foci-forming assay.</p>", "links"=>[], "tags"=>["elisa", "assay"], "article_id"=>201794, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_situ_ELISA_assay_validation_/201794", "title"=>"<i>In situ</i> ELISA assay validation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:29:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/531368"], "description"=>"<p>(<b>A</b>) Huh7.5 were seeded in a 96-well plate and infected with DENV-1, (<b>B</b>) DENV-2 and (<b>C</b>) DENV-3 with an MOI of 4, and (<b>D</b>) DENV-4 with an MOI of 0.1. Interferon-α 2A (100 IU/ml) was used as a positive control and after 72 hours post-infection the ELISA was performed. Data were analyzed using one-way ANOVA followed by Tukey test. Values are mean ± SD of three independent experiments. *p<0.05.</p>", "links"=>[], "tags"=>["extracts", "antiviral"], "article_id"=>201861, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Seaweed_extracts_antiviral_screening_/201861", "title"=>"Seaweed extracts antiviral screening.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:31:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/531470"], "description"=>"<p>Huh7.5 was seeded in a 96-well plate and infected with each strain with a MOI of 4 for 1 h30 min, then the seaweed extracts were added and after 72 hours the ELISA was performed. Data were analyzed using one-way ANOVA followed by Dunnett test. Values are mean ± SD of three independent experiments,*p<0.05.</p>", "links"=>[], "tags"=>["inhibition"], "article_id"=>201967, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DENV_infection_inhibition_for_different_strains_serotypes_/201967", "title"=>"DENV infection inhibition for different strains/serotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:32:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/531569"], "description"=>"<p>(<b>A</b>) Huh 7.5 cells were infected with DENV-4 with an MOI of 0.1 following each treatment (before the infection, −1.5 h; during the infection, 0 h; and after the infection, +1.5 h) at the MNTD, the cells were submitted to the ELISA assay (<b>B</b>) and the supernatant for the foci-forming assay. The dashed line represents the detection limit of the foci-forming assay.</p>", "links"=>[], "tags"=>["studies", "seaweed", "extracts", "a8"], "article_id"=>202059, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Time_of_addition_studies_with_seaweed_extracts_A1_A3_A8_and_A12_/202059", "title"=>"Time of addition studies with seaweed extracts A1, A3, A8 and A12.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:34:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/531624"], "description"=>"<p>(<b>A</b>) Huh 7.5 cells were infected with DENV-4 and treated during the infection in a range of concentration for each seaweed extract. (<b>B</b>) CC50, IC50 and SI were calculated. Mean ± SD of three independent experiments, analyzed by sigmoidal dose response curve (variable slope).</p>", "links"=>[], "tags"=>["seaweed", "extracts", "a8"], "article_id"=>202114, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Dose_response_curve_for_the_seaweed_extracts_A1_A3_A8_and_A12_/202114", "title"=>"Dose response curve for the seaweed extracts A1, A3, A8 and A12.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:35:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/531780"], "description"=>"<p>(<b>A</b>) Binding inhibition assay. (<b>B</b>) Internalization inhibition assay. Data were analyzed using one-way ANOVA followed by Dunnett test. Values are mean ± SD of three independent experiments,*p<0.05.</p>", "links"=>[], "tags"=>["seaweed", "extracts", "a8", "a12", "denv-4"], "article_id"=>202270, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_the_seaweed_extracts_A1_A3_A8_and_A12_on_DENV_4_entry_process_/202270", "title"=>"Effect of the seaweed extracts A1, A3, A8 and A12 on DENV-4 entry process.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2012-12-05 00:37:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/531836"], "description"=>"a<p>CC50∶50% cytotoxic concentration (µg/ml).</p>b<p>MNTD: maximum non- toxic dose based on MTT and Neutral Red assays.</p>c<p>Phaeophyta = Brown seaweeds; Rhodophyta = Red seaweeds; Chlorophyta = Green seaweeds.</p>", "links"=>[], "tags"=>["seaweeds", "antiviral", "cytotoxicity"], "article_id"=>202331, "categories"=>["Virology", "Biochemistry", "Infectious Diseases", "Immunology", "Pharmacology", "Biotechnology"], "users"=>["Andrea Cristine Koishi", "Paula Rodrigues Zanello", "Éverson Miguel Bianco", "Juliano Bordignon", "Claudia Nunes Duarte dos Santos"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0051089.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Marine_seaweeds_selected_for_antiviral_screening_location_of_collection_and_cytotoxicity_activity_in_Huh7_5_cells_/202331", "title"=>"Marine seaweeds selected for antiviral screening, location of collection and cytotoxicity activity in Huh7.5 cells.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2012-12-05 00:38:51"}

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Relative Metric

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