Profiling of Androgen Response in Rainbow Trout Pubertal Testis: Relevance to Male Gonad Development and Spermatogenesis
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Profiling of Androgen Response in Rainbow Trout Pubertal Testis: Relevance to Male Gonad Development and Spermatogenesis", "type"=>"journal", "authors"=>[{"first_name"=>"Antoine D.", "last_name"=>"Rolland", "scopus_author_id"=>"16025538500"}, {"first_name"=>"Aurélie", "last_name"=>"Lardenois", "scopus_author_id"=>"12546190900"}, {"first_name"=>"Anne Sophie", "last_name"=>"Goupil", "scopus_author_id"=>"35208366300"}, {"first_name"=>"Jean Jacques", "last_name"=>"Lareyre", "scopus_author_id"=>"6701608663"}, {"first_name"=>"Rémi", "last_name"=>"Houlgatte", "scopus_author_id"=>"56236723600"}, {"first_name"=>"Frédéric", "last_name"=>"Chalmel", "scopus_author_id"=>"6506567446"}, {"first_name"=>"Florence", "last_name"=>"Le Gac", "scopus_author_id"=>"6701423681"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84871908646", "sgr"=>"84871908646", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0053302", "pmid"=>"23301058", "isbn"=>"1932-6203", "pui"=>"368044705"}, "id"=>"52d6eb99-9de9-32e8-83c2-1f18179135c1", "abstract"=>"The capacity of testicular somatic cells to promote and sustain germ cell differentiation is largely regulated by sexual steroids and notably androgens. In fish species the importance of androgens is emphasized by their ability to induce sex reversal of the developing fries and to trigger spermatogenesis. Here we studied the influence of androgens on testicular gene expression in trout testis using microarrays. Following treatment of immature males with physiological doses of testosterone or 11-ketotestosterone, 418 genes that exhibit changes in expression were identified. Interestingly, the activity of testosterone appeared stronger than that of 11-ketotestosterone. Expression profiles of responsive genes throughout testis development and in isolated germ cells confirmed androgens to mainly affect gene expression in somatic cells. Furthermore, specific clusters of genes that exhibit regulation coincidently with changes in the natural circulating levels of androgens during the reproductive cycle were highlighted, reinforcing the physiological significance of these data. Among somatic genes, a phylogenetic footprinting study identified putative androgen response elements within the proximal promoter regions of 42 potential direct androgen target genes. Finally, androgens were also found to alter the germ line towards meiotic expression profiles, supporting the hypothesis of a role for the somatic responsive genes in driving germ cell fate. This study significantly increases our understanding of molecular pathways regulated by androgens in vertebrates. The highly cyclic testicular development in trout together with functions associated with regulated genes reveal potential mechanisms for androgen actions in tubule formation, steroid production, germ cell development and sperm secretion.", "link"=>"http://www.mendeley.com/research/profiling-androgen-response-rainbow-trout-pubertal-testis-relevance-male-gonad-development-spermatog", "reader_count"=>40, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>4, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>2, "Student > Master"=>6, "Other"=>4, "Student > Bachelor"=>3, "Lecturer"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>3, "Researcher"=>4, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>2, "Student > Master"=>6, "Other"=>4, "Student > Bachelor"=>3, "Lecturer"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>27, "Medicine and Dentistry"=>2, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>27}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>2}}, "reader_count_by_country"=>{"Canada"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84871908646"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84871908646?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871908646&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84871908646&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84871908646", "dc:identifier"=>"SCOPUS_ID:84871908646", "eid"=>"2-s2.0-84871908646", "dc:title"=>"Profiling of Androgen Response in Rainbow Trout Pubertal Testis: Relevance to Male Gonad Development and Spermatogenesis", "dc:creator"=>"Rolland A.D.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-01-03", "prism:coverDisplayDate"=>"3 January 2013", "prism:doi"=>"10.1371/journal.pone.0053302", "citedby-count"=>"26", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Centre INRAE Bretagne-Normandie", "affiliation-city"=>"Le Rheu", "affiliation-country"=>"France"}], "pubmed-id"=>"23301058", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e53302", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0053302", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"1", "year"=>"2013", "pdf_views"=>"41", "xml_views"=>"3", "html_views"=>"340"}
  • {"month"=>"2", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"67"}
  • {"month"=>"3", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"68"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"56"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"99"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"152"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"135"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"96"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"117"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"104"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"93"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"86"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/513876"], "description"=>"<p>Relative expression profiles as measured by qPCR in the androgen supplementation experiment (left panel) and by microarray during spermatogenesis (right panel). The mRNA levels measured by qPCR were normalised to the Rps15 gene reference levels whereas microarray data correspond to Log2-transformed signal intensities. Mean values ± SEM are are presented. One, 2 or 3 star symbols indicate p<0.05, p<0.01 or p<0.005, respectively, when comparing treated samples and corresponding controls (cont) using the non-parametric test of Mann & Whitney. T1 and T2 correspond to animals supplemented with testosterone implants of 0.1 and 0.2 mg, respectively. 11KT indicates animals treated with 11-ketotestosterone implants of 0.25 mg. Day 7 and Day 14 correspond to animals treated for 7 and 14 days, respectively. The number of biological replicates used for qPCR experiments is indicated in brackets. Roman numbers (I–V and VIII) indicate testicular developmental stages analysed by microarrays. Sg = spermatogonia. Sc = spermatocytes. St = spermatids.</p>", "links"=>[], "tags"=>["pcr", "validation"], "article_id"=>184365, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053302.g005", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Real_time_PCR_validation_of_candidate_genes_/184365", "title"=>"Real-time PCR validation of candidate genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:12:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/513613"], "description"=>"<p>Heatmap representation of specific expression clusters of androgen-regulated genes that also display differential expression during trout spermatogenesis. Subgroups of genes were established by crossing 4 clusters (1 to 4) of androgen-responsive genes and 9 clusters (A to I) of spermatogenesis as indicated in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053302#pone-0053302-t001\" target=\"_blank\">table 1</a>. For these genes, we present expression signals obtained: - in testes from controls and androgen-supplemented animals, and - in testes at various developmental stages and in enriched fractions of isolated germ cells. Normalized expression values are shown according to the scale bar while histograms represent averaged fold changes to the control (androgens data) or to the stage I (spermatogenesis data) ± SEM for each cluster. T1 and T2 correspond to animals supplemented with testosterone implants of 0.1 and 0.2 mg, respectively; 11KT indicates animals treated with 11-ketotestosterone implants of 0.25 mg; d7 and d14 correspond to animals treated for 7 and 14 days, respectively. Roman numerals (I–V and VIII) indicate testicular developmental stages. Sg, Sc and St correspond to isolated germ cell populations enriched in spermatogonia, spermatocytes and spermatids, respectively. The annotation of each gene is accessible in the searchable File S1.</p>", "links"=>[], "tags"=>["androgen-responsive", "developmentally-regulated"], "article_id"=>184104, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053302.g002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_androgen_responsive_and_developmentally_regulated_genes_/184104", "title"=>"Expression of androgen-responsive and developmentally-regulated genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:08:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/514041"], "description"=>"<p>Subgroups of clones were established by crossing 4 clusters of androgen-responsive genes (1 to 4, present study) and 9 clusters (A to I) of genes differentially expressed during trout spermatogenetic development <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053302#pone.0053302-Rolland2\" target=\"_blank\">[23]</a>. Bolded numbers indicate subgroups of genes that are represented in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053302#pone-0053302-g002\" target=\"_blank\">Figure 2</a>.</p>", "links"=>[], "tags"=>["androgen-responsive", "clones", "differential"], "article_id"=>184533, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053302.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_androgen_responsive_clones_that_also_exhibit_differential_expression_during_spermatogenesis_/184533", "title"=>"Summary of androgen-responsive clones that also exhibit differential expression during spermatogenesis.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-01-03 01:15:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/513723"], "description"=>"<p>A : The relative number of genes displaying 0 to 14 conserved AREs is presented. Conserved AREs (cAREs) were searched in a region of −10000/+2000 base pairs relative to the TSS in the promoter of 395 androgen-responsive gene orthologs in <i>Gasterosteus aculeatus</i>. B : Frequency of conserved AREs in the promoter regions of androgen-responsive genes with respect to the TSS, expressed by number of sites per kilobase of DNA.</p>", "links"=>[], "tags"=>["conserved", "androgen", "elements", "promoter", "androgen-regulated"], "article_id"=>184207, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053302.g003", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identification_of_conserved_Androgen_Response_Elements_within_the_promoter_of_androgen_regulated_genes_/184207", "title"=>"Identification of conserved Androgen Response Elements within the promoter of androgen-regulated genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:10:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/513780"], "description"=>"<p>Over-represented “biological process” terms from the GeneOntology (GO) were identified in the 4 expression clusters of androgen-responsive genes (1 to 4) as well as in 3 groups of androgen-regulated genes that exhibit “somatic” (A to D), “spermatogonial” (E and F) and “germline” (G to I) expression profiles during spermatogenesis. Rectangles indicate the observed (left) and expected (right) numbers of genes bearing the corresponding GO term whereas the number of genes exhibiting this GO term on the entire microarray is given on the left. Only GO terms with a p-value of ≤10<sup>−6</sup> and for which at least 3 non-redundant genes were in the cluster were considered as statistically enriched. To avoid redundancy between closely related terms an Ontology Specific Information Rate (OSIR) cutoff of ≥0.95 was selected <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053302#pone.0053302-Chalmel1\" target=\"_blank\">[27]</a>. Numbers in bold indicate a statistical enrichment for a given GO term according to the scale bar.</p>", "links"=>[], "tags"=>["processes", "testicular", "androgen-responsive"], "article_id"=>184270, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053302.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Enriched_biological_processes_among_testicular_androgen_responsive_genes_/184270", "title"=>"Enriched biological processes among testicular androgen-responsive genes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:11:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/513536"], "description"=>"<p>A : Increased androgen plasma concentrations following testosterone and 11-ketotestosterone supplementation. Androgen circulating levels were measured in stage I to II males supplemented for 7 or 14 days with testosterone (0.1 mg, T1; 0.2 mg, T2) or 11-ketotestosterone (0.25 mg, 11KT) as well as in corresponding controls (Cont). B : Natural testosterone (grey) and 11-ketotestosterone (black) circulating levels throughout the male trout reproductive cycle. Roman numerals (I-VIII) indicate testicular developmental stages. Mean values ± standard deviation are presented.</p>", "links"=>[], "tags"=>["plasma", "levels"], "article_id"=>184028, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053302.g001", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Androgen_plasma_levels_in_male_trout_/184028", "title"=>"Androgen plasma levels in male trout.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:07:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/513972"], "description"=>"<p>Real-time PCR validation of candidate genes (continued).</p>", "links"=>[], "tags"=>["pcr", "validation", "genes"], "article_id"=>184463, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0053302.g006", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Real_time_PCR_validation_of_candidate_genes_continued_/184463", "title"=>"Real-time PCR validation of candidate genes (continued).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-01-03 01:14:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/278872", "https://ndownloader.figshare.com/files/278903", "https://ndownloader.figshare.com/files/278955", "https://ndownloader.figshare.com/files/278995", "https://ndownloader.figshare.com/files/279048", "https://ndownloader.figshare.com/files/279081", "https://ndownloader.figshare.com/files/279135", "https://ndownloader.figshare.com/files/279181", "https://ndownloader.figshare.com/files/279208"], "description"=>"<div><p>The capacity of testicular somatic cells to promote and sustain germ cell differentiation is largely regulated by sexual steroids and notably androgens. In fish species the importance of androgens is emphasized by their ability to induce sex reversal of the developing fries and to trigger spermatogenesis. Here we studied the influence of androgens on testicular gene expression in trout testis using microarrays. Following treatment of immature males with physiological doses of testosterone or 11-ketotestosterone, 418 genes that exhibit changes in expression were identified. Interestingly, the activity of testosterone appeared stronger than that of 11-ketotestosterone. Expression profiles of responsive genes throughout testis development and in isolated germ cells confirmed androgens to mainly affect gene expression in somatic cells. Furthermore, specific clusters of genes that exhibit regulation coincidently with changes in the natural circulating levels of androgens during the reproductive cycle were highlighted, reinforcing the physiological significance of these data. Among somatic genes, a phylogenetic footprinting study identified putative androgen response elements within the proximal promoter regions of 42 potential direct androgen target genes. Finally, androgens were also found to alter the germ line towards meiotic expression profiles, supporting the hypothesis of a role for the somatic responsive genes in driving germ cell fate. This study significantly increases our understanding of molecular pathways regulated by androgens in vertebrates. The highly cyclic testicular development in trout together with functions associated with regulated genes reveal potential mechanisms for androgen actions in tubule formation, steroid production, germ cell development and sperm secretion.</p> </div>", "links"=>[], "tags"=>["profiling", "androgen", "rainbow", "trout", "pubertal", "relevance", "gonad", "spermatogenesis"], "article_id"=>114995, "categories"=>["Chemistry", "Inorganic Chemistry", "Biochemistry", "Physiology", "Genetics"], "users"=>["Antoine D. Rolland", "Aurélie Lardenois", "Anne-Sophie Goupil", "Jean-Jacques Lareyre", "Rémi Houlgatte", "Frédéric Chalmel", "Florence Le Gac"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0053302.s001", "https://dx.doi.org/10.1371/journal.pone.0053302.s002", "https://dx.doi.org/10.1371/journal.pone.0053302.s003", "https://dx.doi.org/10.1371/journal.pone.0053302.s004", "https://dx.doi.org/10.1371/journal.pone.0053302.s005", "https://dx.doi.org/10.1371/journal.pone.0053302.s006", "https://dx.doi.org/10.1371/journal.pone.0053302.s007", "https://dx.doi.org/10.1371/journal.pone.0053302.s008", "https://dx.doi.org/10.1371/journal.pone.0053302.s009"], "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Profiling_of_Androgen_Response_in_Rainbow_Trout_Pubertal_Testis_Relevance_to_Male_Gonad_Development_and_Spermatogenesis__/114995", "title"=>"Profiling of Androgen Response in Rainbow Trout Pubertal Testis: Relevance to Male Gonad Development and Spermatogenesis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-01-03 01:23:15"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"28", "full-text"=>"25", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"72", "cited-by"=>"0", "year"=>"2013", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"26", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"19", "full-text"=>"22", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"18", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"16", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[248, 440, 562, 666, 765, 856, 944, 1028, 1112, 1198, 1282, 1362, 1430]}, {"subject_area"=>"/Biology and life sciences/Anatomy and physiology", "average_usage"=>[256, 428]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[266, 468, 593, 703, 804, 903, 993, 1084, 1171, 1256, 1339, 1422, 1492]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[281, 484, 611, 728, 835, 934, 1030, 1123, 1214, 1299, 1383, 1464]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[256, 449, 572, 676, 775, 866, 955, 1041, 1127, 1213, 1290, 1370, 1437]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[248, 441, 564, 668, 769, 859, 948, 1034, 1117, 1202, 1290, 1368, 1437]}]}
Loading … Spinner
There are currently no alerts