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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/975534"], "description"=>"<p>The spectrum was measured directly under the room light which had an opaque cover (blue line) and again in the cage (red line), which did not receive any direct room illumination. The measurements for the cage are X 10 of those for the room light. As common with room lighting, it is primarily composed of a series of discrete peaks, however none of these are close to 670 nm.</p>", "links"=>[], "tags"=>["mice"], "article_id"=>643238, "categories"=>["Physiology", "Neuroscience", "Immunology"], "users"=>["Rana Begum", "Michael B. Powner", "Natalie Hudson", "Chris Hogg", "Glen Jeffery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057828.g002", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spectral_composition_of_room_light_in_which_mice_were_caged_/643238", "title"=>"Spectral composition of room light in which mice were caged.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-01 17:18:53"}
• {"files"=>["https://ndownloader.figshare.com/files/975580"], "description"=>"<p>A.B. Retinal sections stained with C3 (red). This accumulates on Bruch’s membrane and outer segments. C.D Following 670 nm treatment C3 was significantly reduced on Bruch’s membrane and photoreceptor outer segments (p = 0.0001 for each). E. These data were confirmed with qPCR analysis, which showed again a statistically significant reduction in C3 expression following treatment (p = 0.0031). Abbreviations, Bruch’s membrane (BM), photoreceptor (PR), complement component (C3). Scale bars = 40 µm.</p>", "links"=>[], "tags"=>["retinal", "inflammation", "reduced", "670", "nm"], "article_id"=>643278, "categories"=>["Physiology", "Neuroscience", "Immunology"], "users"=>["Rana Begum", "Michael B. Powner", "Natalie Hudson", "Chris Hogg", "Glen Jeffery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057828.g005", "stats"=>{"downloads"=>1, "page_views"=>34, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Outer_retinal_inflammation_is_significantly_reduced_following_670_nm_treatment_/643278", "title"=>"Outer retinal inflammation is significantly reduced following 670 nm treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-01 17:23:55"}
• {"files"=>["https://ndownloader.figshare.com/files/975576"], "description"=>"<p>A–D. RPE flat mounts labelled with IBA-1 (green) to identify macrophages. After 14 days of treatment with 670 nm light these cells had significantly altered morphology over a range of metrics. E. Number of IBA-1⁺ cells per eye was measured; there was a reduction in the number of macrophages following treatment, but this was not statistically significant. F,G. Dendritic process length and area were significantly increased by 670 nm light (27%, 28% respectively) following treatment (p = 0.0001 for each). H. The distance between macrophages was measured from nucleus of one cell to its nearest neighbour, which also showed a significant increase (p = 0.0001). I. Not only were the 670 nm treated cells larger they also had more primary processes (p = 0.05). J. Even though these cells had a greater dendritic field and territory they had smaller cell bodies in comparison to controls (p = 0.05). Abbreviations, retinal pigmented epithelial (RPE), ionized calcium-binding adaptor molecule 1 (IBA-1), macrophages (mφ). Scale bars A, B = 40 µm, C,D = 20 µm.</p>", "links"=>[], "tags"=>["staining", "showed", "macrophage", "morphology", "670", "nm"], "article_id"=>643274, "categories"=>["Physiology", "Neuroscience", "Immunology"], "users"=>["Rana Begum", "Michael B. Powner", "Natalie Hudson", "Chris Hogg", "Glen Jeffery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057828.g004", "stats"=>{"downloads"=>1, "page_views"=>175, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_IBA_1_staining_showed_significantly_different_macrophage_morphology_between_670_nm_and_control_groups_/643274", "title"=>"IBA-1 staining showed significantly different macrophage morphology between 670 nm and control groups.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-01 17:23:03"}
• {"files"=>["https://ndownloader.figshare.com/files/975557"], "description"=>"<p>A–D. Retinal sections were immunostained with COX (red). This enzyme is involved in mitochondrial respiratory chain and is activated by 670 nm light. In the experimental groups COX expression is significantly up regulated (A–B) compared to controls (C–E, p = 0.0001). B, D. Showed label with DAPI as a counter stain. COX staining was largely confined to photoreceptor inner segments and outer plexiform layer. F–G. This result was confirmed with significant increases in COX expression in qPCR (p = 0.0030) and Western blots (p = 0.0004). H. Differences for tubulin between groups were non-significant. Abbreviations, cytochrome c oxidase (COX), photoreceptor (PR), outer nuclear layer (ONL) and outer plexiform layer (OPL). Scale bar A–D = 40 µm.</p>", "links"=>[], "tags"=>["cytochrome", "oxidase", "enhanced", "670", "nm", "treated", "aged"], "article_id"=>643255, "categories"=>["Physiology", "Neuroscience", "Immunology"], "users"=>["Rana Begum", "Michael B. Powner", "Natalie Hudson", "Chris Hogg", "Glen Jeffery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057828.g003", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mitochondrial_cytochrome_c_oxidase_expression_is_enhanced_in_670_nm_treated_aged_mice_/643255", "title"=>"Mitochondrial cytochrome c oxidase expression is enhanced in 670 nm treated aged mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-01 17:20:53"}
• {"files"=>["https://ndownloader.figshare.com/files/975590"], "description"=>"<p>Vimentin and GFAP are Muller cell markers, which are up regulated in ageing and when the retina is damaged or stressed. A–D. 670 nm treated groups showed significantly reduced vimentin labelling both in terms of length of Muller cells processes (E, p = 0.0001) and their number (F, p = 0.0329). G–J. Shows similar differences for GFAP with a statistically significant reduction following 670 nm treatment (K, p = 0.0001). In both A–D and G–J upper panels are immunostaining alone (red) while in lower panels DAPI is shown as a counter stain (blue). Abbreviations, outer nuclear layer (ONL), outer plexiform layer (OPL), inner plexiform layer (IPL), ganglion cell layer (GCL) and glial fibrillary acidic protein (GFAP). Scale bar A–D = 40 µm.</p>", "links"=>[], "tags"=>["reduced", "670", "nm"], "article_id"=>643288, "categories"=>["Physiology", "Neuroscience", "Immunology"], "users"=>["Rana Begum", "Michael B. Powner", "Natalie Hudson", "Chris Hogg", "Glen Jeffery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057828.g006", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Retinal_stress_is_significantly_reduced_following_670_nm_treatment_/643288", "title"=>"Retinal stress is significantly reduced following 670 nm treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-01 17:26:10"}
• {"files"=>["https://ndownloader.figshare.com/files/975529"], "description"=>"<p>LED lights were placed at both ends of the cage behind perspex screens. The internal space occupied a volume of 6,422 cubic centimeters (13 cm×26 cm×19 cm). A maximum of five animals were caged together. The longest distance the animals were from the light source was approximately 13 cm and the minimum was 0.4 cm, which is the thickness of the perspex screens.</p>", "links"=>[], "tags"=>["670", "nm"], "article_id"=>643233, "categories"=>["Physiology", "Neuroscience", "Immunology"], "users"=>["Rana Begum", "Michael B. Powner", "Natalie Hudson", "Chris Hogg", "Glen Jeffery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057828.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cage_for_670_nm_exposure_/643233", "title"=>"Cage for 670 nm exposure.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-01 17:18:11"}
• {"files"=>["https://ndownloader.figshare.com/files/975597"], "description"=>"<p>A.B. Aβ (red) expression was measured at the Bruch’s membrane/RPE interface and this was unchanged between experimental and control groups as shown in C. Abbreviation, amyloid beta (Aβ), Bruch’s membrane (BM), retinal pigmented epithelium (RPE). Scale bar A–B = 40 µm.</p>", "links"=>[], "tags"=>["beta", "unaffected", "670", "nm"], "article_id"=>643295, "categories"=>["Physiology", "Neuroscience", "Immunology"], "users"=>["Rana Begum", "Michael B. Powner", "Natalie Hudson", "Chris Hogg", "Glen Jeffery"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057828.g007", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amyloid_beta_expression_is_unaffected_by_670_nm_treatment_/643295", "title"=>"Amyloid beta expression is unaffected by 670 nm treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-01 17:27:18"}

PMC Usage Stats | Further Information

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Relative Metric

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F1000Prime