Reference Genes to Study Herbicide Stress Response in Lolium sp.: Up-Regulation of P450 Genes in Plants Resistant to Acetolactate-Synthase Inhibitors
- Publication Date
- May 16, 2013
- Journal
- PLOS ONE
- Authors
- Arnaud Duhoux & Christophe Délye
- Volume
- 8
- Issue
- 5
- Pages
- e63576
- DOI
- https://dx.plos.org/10.1371/journal.pone.0063576
- Publisher URL
- http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0063576
- PubMed
- http://www.ncbi.nlm.nih.gov/pubmed/23696834
- PubMed Central
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3656029
- Europe PMC
- http://europepmc.org/abstract/MED/23696834
- Web of Science
- 000319081900042
- Scopus
- 84877810067
- Mendeley
- http://www.mendeley.com/research/reference-genes-study-herbicide-stress-response-lolium-sp-upregulation-p450-genes-plants-resistant-a
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Mendeley | Further Information
{"title"=>"Reference Genes to Study Herbicide Stress Response in Lolium sp.: Up-Regulation of P450 Genes in Plants Resistant to Acetolactate-Synthase Inhibitors", "type"=>"journal", "authors"=>[{"first_name"=>"Arnaud", "last_name"=>"Duhoux", "scopus_author_id"=>"56633292600"}, {"first_name"=>"Christophe", "last_name"=>"Délye", "scopus_author_id"=>"6701632994"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "sgr"=>"84877810067", "scopus"=>"2-s2.0-84877810067", "isbn"=>"1932-6203 (Electronic)\r1932-6203 (Linking)", "pui"=>"368926645", "doi"=>"10.1371/journal.pone.0063576", "pmid"=>"23696834"}, "id"=>"17f46876-8ee3-3b82-80bc-8aeb89db4b69", "abstract"=>"<p>Variation in the expression of numerous genes is at the basis of plant response to environmental stresses. Non-target-site-based resistance to herbicides (NTSR), the major threat to grass weed chemical control, is governed by a subset of the genes involved in herbicide stress response. Quantitative PCR assays allowing reliable comparison of gene expression are thus key to identify genes governing NTSR. This work aimed at identifying a set of reference genes with a stable expression to be used as an internal standard for the normalisation of quantitative PCR data in studies investigating NTSR to herbicides inhibiting acetolactate synthase (ALS) in the major grass weed <italic>Lolium</italic> sp. Gene expression stability was assessed in plants resistant or sensitive to two ALS inhibitors, subjected or not to herbicide stress. Using three complementary approaches implemented in the programs BestKeeper, NormFinder and geNorm, cap-binding protein, glyceraldehyde-3-phosphate-dehydrogenase and ubiquitin were identified as the most suitable reference genes. This reference gene set can probably be used to study herbicide response in other weed species. It was used to compare the expression of the genes encoding two herbicide target enzymes (ALS and acetyl-coenzyme A carboxylase) and five cytochromes P450 (CYP) with potential herbicide-degrading activity between plants resistant or sensitive to ALS inhibitors. Overall, herbicide application enhanced <italic>CYP</italic> gene expression. Constitutive up-regulation of all <italic>CYP</italic> genes observed in resistant plants compared to sensitive plants suggested enhanced secondary metabolism in the resistant plants. Comprehensive transcriptome studies associated to gene expression analyses using the reference gene set validated here are required to unravel NTSR genetic determinants.</p>", "link"=>"http://www.mendeley.com/research/reference-genes-study-herbicide-stress-response-lolium-sp-upregulation-p450-genes-plants-resistant-a", "reader_count"=>32, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Other"=>2, "Student > Master"=>5, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>2, "Other"=>2, "Student > Master"=>5, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>28, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>28}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Environmental Science"=>{"Environmental Science"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}CrossRef
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Scopus | Further Information
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Relative Metric
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