Opto-Mechanical Coupling in Interfaces under Static and Propagative Conditions and Its Biological Implications
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{"title"=>"Opto-Mechanical Coupling in Interfaces under Static and Propagative Conditions and Its Biological Implications", "type"=>"journal", "authors"=>[{"first_name"=>"Shamit", "last_name"=>"Shrivastava", "scopus_author_id"=>"55785593600"}, {"first_name"=>"Matthias F.", "last_name"=>"Schneider", "scopus_author_id"=>"57199120501"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84879826719", "issn"=>"19326203", "scopus"=>"2-s2.0-84879826719", "pmid"=>"23861769", "pui"=>"369260693", "doi"=>"10.1371/journal.pone.0067524"}, "id"=>"84798333-f05c-3d12-a93e-a7efa189199f", "abstract"=>"Fluorescent dyes are vital for studying static and dynamic patterns and pattern formation in cell biology. Emission properties of the dyes incorporated in a biological interface are known to be sensitive to their local environment. We report that the fluorescence intensity of dye molecules embedded in lipid interfaces is indeed a thermodynamic observable of the system. Opto-mechanical coupling of lipid-dye system was measured as a function of the thermodynamic state of the interface. The corresponding state diagrams quantify the thermodynamic coupling between intensity I and lateral pressure π. We further demonstrate that the coupling is conserved upon varying the temperature T. Notably, the observed opto-mechanical coupling is not limited to equilibrium conditions, but also holds for propagating pressure pulses. The non-equilibrium data show, that fluorescence is especially sensitive to dynamic changes in state such as the LE-LC phase transition. We conclude that variations in the thermodynamic state (here π and T, in general pH, membrane potential V, etc also) of lipid membranes are capable of controlling fluorescence intensity. Therefore, interfacial thermodynamic state diagrams of I should be obtained for a proper interpretation of intensity data.", "link"=>"http://www.mendeley.com/research/optomechanical-coupling-interfaces-under-static-propagative-conditions-biological-implications", "reader_count"=>19, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>9, "Student > Master"=>2, "Other"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>9, "Student > Master"=>2, "Other"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>1, "Medicine and Dentistry"=>1, "Neuroscience"=>1, "Design"=>1, "Physics and Astronomy"=>9}, "reader_count_by_subdiscipline"=>{"Design"=>{"Design"=>1}, "Engineering"=>{"Engineering"=>4}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>9}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>2}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1112055"], "description"=>"<p>As a visual aid for beginners, the figure introduces the thermodynamic approach for a propagating pulse at the interface. A perturbation at the interface alters the state of local hydrated environment. The disturbance can propagate and is conserved over long distances influencing the properties of a single molecule (e.g the emission properties of a flourophore, kinetics of an enzyme) located at a remote location. All changes induced are reversible leading to local oscillations in the mean states the wave passes by. In a nonlinear system, the thermodynamic properties can change with a propagating density pulse (I – II). The accompanied state change implies changes in local fluctuations and consequently kinetic processes. Here <i>X<sub>j</sub></i> can be pressure, temperature, electric field etc and <i>n<sub>i</sub></i> can be area, charge, intensity, [H<sup>+</sup>] etc.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles", "visualization", "propagation"], "article_id"=>740218, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g009", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Approximate_visualization_of_Pulse_Propagation_at_an_Interface_/740218", "title"=>"Approximate visualization of Pulse Propagation at an Interface.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112050"], "description"=>"<p>A) Opto-mechanical susceptibilities of a DMPC-NBD monolayer. The local temperature at the site for optical detection is the same as the average temperature of the monolayer. Consequently the peaks in the corresponding susceptibilities correlate. B) When measured in a region of higher temperature (region 5 in Fig. 1) the opto-mechanic susceptibility (green-dash curve) is shifted against the compressibility (blue-solid curve) by 1.5 mN/m. From the given temperature dependence a local temperature increase by 0.8C (7.78.5C) is expected, which is in perfect agreement with the temperature difference measured during calibration (Fig. 1). The figure in the inset plots a linear regression on transition pressure for isotherms at different temperatures.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles", "reports", "variations"], "article_id"=>740213, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g004", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Intensity_reports_local_variations_in_the_mechanical_properties_of_the_interface_/740213", "title"=>"Intensity reports local variations in the mechanical properties of the interface.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112046"], "description"=>"<p>Opto-mechanical susceptibility (green-dash) and compressibility (blue – solid), plotted as a function of pressure. The original intensity and area curves have been plotted as a function of lateral pressure in the inset. Area and intensity change nonlinearly as a function of pressure during the LE-LC transition and the peaks in the corresponding susceptibilities (y axis) correlate precisely. The peak in opto-mechanical susceptibility results in increased sensitivity of intensity to pressure variations around LE-LC transition.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles", "isotherm", "quasi-static"], "article_id"=>740209, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g002", "stats"=>{"downloads"=>2, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Opto_Mechanical_Isotherm_for_a_Quasi_Static_Process_/740209", "title"=>"Opto-Mechanical Isotherm for a Quasi-Static Process.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112044"], "description"=>"<p>(1) The lateral pressure is measured using a Wilhemly balance. (2) The monolayer is excited chemically using a dipper that can be moved vertically. (3) Fluorescence is measured simultaneously with pressure. The spot for fluorescence measurement (D = 400 µm) can be moved across the interface to measure fluorescence from different regions (For simultaneous optical and mechanical measurement of a pressure pulse, (1) and (3) were equidistant from (2) at 12cm). (4) The barriers can be moved horizontally to compress the monolayer at the air/water interface. The temperature in the bulk can be controlled from underneath by circulating water from a heat bath (solid black). (5) There is a glass window which introduces local temperature gradients due to the absence of circulating coolant underneath. This gradient was used to investigate the local effects of temperature on the monolayer. At a room temperature of 21°C, on cooling down, the temperature at ‘5′ was 8.5°C compared to 7.7°C in the rest of the trough. The contact of solvent with the monolayer created a trigger signal that was used to control the microscope LED and data acquisition.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles"], "article_id"=>740207, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Experimental_setup_/740207", "title"=>"Experimental setup.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112057", "https://ndownloader.figshare.com/files/1112058", "https://ndownloader.figshare.com/files/1112059", "https://ndownloader.figshare.com/files/1112060", "https://ndownloader.figshare.com/files/1112061"], "description"=>"<div><p>Fluorescent dyes are vital for studying static and dynamic patterns and pattern formation in cell biology. Emission properties of the dyes incorporated in a biological interface are known to be sensitive to their local environment. We report that the fluorescence intensity of dye molecules embedded in lipid interfaces is indeed a thermodynamic observable of the system. Opto-mechanical coupling of lipid-dye system was measured as a function of the thermodynamic state of the interface. The corresponding state diagrams quantify the thermodynamic coupling between intensity <i>I</i> and lateral pressure <i>π</i>. We further demonstrate that the coupling is conserved upon varying the temperature <i>T</i>. Notably, the observed opto-mechanical coupling is <i>not</i> limited to equilibrium conditions, but also holds for propagating pressure pulses. The non-equilibrium data show, that fluorescence is especially sensitive to dynamic changes in state such as the LE-LC phase transition. We conclude that variations in the thermodynamic state (here <i>π</i> and <i>T,</i> in general <i>pH, membrane potential V</i>, etc also) of lipid membranes are capable of controlling fluorescence intensity. Therefore, interfacial thermodynamic state diagrams of <i>I</i> should be obtained for a proper interpretation of intensity data.</p></div>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles", "coupling", "interfaces", "static", "propagative"], "article_id"=>740220, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0067524.s001", "https://dx.doi.org/10.1371/journal.pone.0067524.s002", "https://dx.doi.org/10.1371/journal.pone.0067524.s003", "https://dx.doi.org/10.1371/journal.pone.0067524.s004", "https://dx.doi.org/10.1371/journal.pone.0067524.s005"], "stats"=>{"downloads"=>7, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Opto_Mechanical_Coupling_in_Interfaces_under_Static_and_Propagative_Conditions_and_Its_Biological_Implications_/740220", "title"=>"Opto-Mechanical Coupling in Interfaces under Static and Propagative Conditions and Its Biological Implications", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112054"], "description"=>"<p>Opto-mechanical coupling showing different transitions in a DPPC/NBD system at 22 C. The opto-mechanical isotherm is remarkably similar to a surface potential isotherm for DPPC monolayer. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0067524#pone.0067524-Vogel1\" target=\"_blank\">[32]</a> Specifically, the abrupt increase in intensity and the dipole moment during gas-LE transition are precisely aligned and support the dipole based explanation for the microscopic origin of the coupling.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles", "microscopic", "opto-mechanical"], "article_id"=>740217, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g008", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_On_the_Microscopic_origin_of_the_Opto_mechanical_coupling_/740217", "title"=>"On the Microscopic origin of the Opto-mechanical coupling.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112052"], "description"=>"<p>. The state of the initial equilibrium is marked on the pressure intensity isotherm in top left. Time profile of intensity (green-dashed) v/s pressure (blue-solid) pulses is plotted in A) LC, B) LE-LC and C) LE region. In the LE region intensity moderately couples to a propagating pressure pulse. Intensity is most sensitive to small pressure variations in LE-LC region. This non-equilibrium behavior is qualitatively similar to the quasi-static coupling. The opto-mechanical coupling under non-equilibrium conditions suddenly disappears in the LC regions where the intensity hardly responds to much stronger pressure variations. In (B) the pulse arrives at Δt = 0.44s at a distance of 12cm giving a velocity of propagation c = 0.27 m/s. Experiments were performed in a DPPC/NBD-PE (1%) system at 21°C.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles"], "article_id"=>740215, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Optical_measurement_of_a_Pressure_Pulse_/740215", "title"=>"Optical measurement of a Pressure Pulse", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112053"], "description"=>"<p>Non-equilibrium opto-mechanical susceptibility (green) as obtained from differential changes in pressure and intensity during individual pulses. The corresponding opto-mechanical susceptibility under equilibrium conditions is plotted in blue. Peak in equilibrium as well as non equilibrium conditions appear around 7 mN/m. Both fits were obtained assuming a sigmoidal response of intensity with respect to pressure in the transition region. The fit quality therefore gets worse away from the transition as the priority was to resolve the peak.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles", "coupling", "non", "equilibrium", "processes"], "article_id"=>740216, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g007", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Opto_Mechanical_Coupling_for_Non_Equilibrium_Processes_Propagating_Pulses_/740216", "title"=>"Opto-Mechanical Coupling for Non Equilibrium Processes (Propagating Pulses).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112051"], "description"=>"<p>The surface can be broadly divided in to three regions as liquid expanded (LE), liquid expanded – liquid condensed (LE-LC) and liquid condensed (LC) region. Slopes along the <i>A</i> axis represent compressibility and area fluctuations, while slopes along <i>I</i> characterize opto-mechanical susceptibility and fluctuations in Intensity, respectively. The transition region (LE-LC) moves to higher pressure, lower area and lower intensity with increasing temperature. The curves represent real data acquired using DPPC-NBD.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles"], "article_id"=>740214, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g005", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Opto_Mechanical_State_diagram_/740214", "title"=>"Opto-Mechanical State diagram.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/1112047"], "description"=>"<p><i>(</i>Δ<i>I/I)<sub>Tr</sub></i> plotted against <i>(</i>Δ<i>A/A)<sub>Tr</sub></i> for LE-LC transitions at different temperatures for a DPPC/NBD-PE system. The proportionality between intensity and area is invariant of the temperature. The numerator of the fraction is the total change in the variable during LE-LC transition where as the denominator represents the mean value of the variable during the transition. This also means that intensity like surface area or density is an observable of the interface.</p>", "links"=>[], "tags"=>["Biochemistry", "biocatalysis", "immunology", "Immunologic techniques", "immunofluorescence", "Molecular cell biology", "Signal transduction", "Mechanisms of signal transduction", "Chemical reactions", "catalysis", "Physical chemistry", "thermodynamics", "Material by structure", "Material films", "biophysics", "Biophysics theory", "Physical laws and principles"], "article_id"=>740210, "categories"=>["Physics", "Medicine", "Chemistry", "Biological Sciences"], "users"=>["Shamit Shrivastava", "Matthias F. Schneider"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0067524.g003", "stats"=>{"downloads"=>3, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Opto_mechanical_Invariance_/740210", "title"=>"Opto-mechanical Invariance.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-04 02:53:57"}

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Relative Metric

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