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{"title"=>"Tumor-specific chromosome mis-segregation controls cancer plasticity by maintaining tumor heterogeneity", "type"=>"journal", "authors"=>[{"first_name"=>"Yuanjie", "last_name"=>"Hu", "scopus_author_id"=>"55661652500"}, {"first_name"=>"Ning", "last_name"=>"Ru", "scopus_author_id"=>"6603322237"}, {"first_name"=>"Huasheng", "last_name"=>"Xiao", "scopus_author_id"=>"57199793549"}, {"first_name"=>"Abhishek", "last_name"=>"Chaturbedi", "scopus_author_id"=>"51863108800"}, {"first_name"=>"Neil T.", "last_name"=>"Hoa", "scopus_author_id"=>"7003956008"}, {"first_name"=>"Xiao Jun", "last_name"=>"Tian", "scopus_author_id"=>"57189358201"}, {"first_name"=>"Hang", "last_name"=>"Zhang", "scopus_author_id"=>"55737254100"}, {"first_name"=>"Chao", "last_name"=>"Ke", "scopus_author_id"=>"54889907500"}, {"first_name"=>"Fengrong", "last_name"=>"Yan", "scopus_author_id"=>"57198405556"}, {"first_name"=>"Jodi", "last_name"=>"Nelson", "scopus_author_id"=>"7404796266"}, {"first_name"=>"Zhenzhi", "last_name"=>"Li", "scopus_author_id"=>"56080327200"}, {"first_name"=>"Robert", "last_name"=>"Gramer", "scopus_author_id"=>"56026346700"}, {"first_name"=>"Liping", "last_name"=>"Yu", "scopus_author_id"=>"55686824900"}, {"first_name"=>"Eric", "last_name"=>"Siegel", "scopus_author_id"=>"7201739716"}, {"first_name"=>"Xiaona", "last_name"=>"Zhang", "scopus_author_id"=>"56080553300"}, {"first_name"=>"Zhenyu", "last_name"=>"Jia", "scopus_author_id"=>"12140153200"}, {"first_name"=>"Martin R.", "last_name"=>"Jadus", "scopus_author_id"=>"7004619374"}, {"first_name"=>"Charles L.", "last_name"=>"Limoli", "scopus_author_id"=>"7003719352"}, {"first_name"=>"Mark E.", "last_name"=>"Linskey", "scopus_author_id"=>"7003806610"}, {"first_name"=>"Jianhua", "last_name"=>"Xing", "scopus_author_id"=>"7202559169"}, {"first_name"=>"Yi Hong", "last_name"=>"Zhou", "scopus_author_id"=>"16168171300"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84896731586", "pmid"=>"24282558", "doi"=>"10.1371/journal.pone.0080898", "pui"=>"372426113", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "issn"=>"19326203", "scopus"=>"2-s2.0-84896731586"}, "id"=>"e49420cb-4dd7-36b0-8201-e51a4fbbd758", "abstract"=>"Aneuploidy with chromosome instability is a cancer hallmark. We studied chromosome 7 (Chr7) copy number variation (CNV) in gliomas and in primary cultures derived from them. We found tumor heterogeneity with cells having Chr7-CNV commonly occurs in gliomas, with a higher percentage of cells in high-grade gliomas carrying more than 2 copies of Chr7, as compared to low-grade gliomas. Interestingly, all Chr7-aneuploid cell types in the parental culture of established glioma cell lines reappeared in single-cell-derived subcultures. We then characterized the biology of three syngeneic glioma cultures dominated by different Chr7-aneuploid cell types. We found phenotypic divergence for cells following Chr7 mis-segregation, which benefited overall tumor growth in vitro and in vivo. Mathematical modeling suggested the involvement of chromosome instability and interactions among cell subpopulations in restoring the optimal equilibrium of tumor cell types. Both our experimental data and mathematical modeling demonstrated that the complexity of tumor heterogeneity could be enhanced by the existence of chromosomes with structural abnormality, in addition to their mis-segregations. Overall, our findings show, for the first time, the involvement of chromosome instability in maintaining tumor heterogeneity, which underlies the enhanced growth, persistence and treatment resistance of cancers.", "link"=>"http://www.mendeley.com/research/tumorspecific-chromosome-missegregation-controls-cancer-plasticity-maintaining-tumor-heterogeneity", "reader_count"=>14, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>2, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>3, "Medicine and Dentistry"=>4, "Physics and Astronomy"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "Portugal"=>1}, "group_count"=>0}

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• http://doi.org/10.1002/em.21988
• http://doi.org/10.1002/cam4.606
• http://doi.org/10.29252/arakmu.11.3.1
• http://doi.org/10.3390/cancers9120168
• http://doi.org/10.3390/cancers11081083
• http://doi.org/10.1016/j.intimp.2014.06.040

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• 4359233
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• {"type"=>"COMMENT", "annotationUri"=>"info:doi/10.1371/annotation/877aec12-3b01-4937-aced-2d48aa8425b5", "title"=>"similar report in yeast", "body"=>"Similar phenomenon of aneuploidy induced phenotypic switch has been reported in yeast\n\nhttp://www.pnas.org/content/early/2013/06/26/1301047110", "isRemoved"=>false, "created"=>"2014-05-11T01:18:14Z", "lastModified"=>"2014-05-11T01:18:14Z", "creator"=>{"userId"=>"128113"}, "highlightedText"=>"", "competingInterestStatement"=>{"creatorWasPrompted"=>true, "hasCompetingInterests"=>false}, "parentArticle"=>{"doi"=>"info:doi/10.1371/journal.pone.0080898", "state"=>"published", "journals"=>{"PLoSONE"=>{"journalKey"=>"PLoSONE", "eIssn"=>"1932-6203", "title"=>"PLOS ONE"}}}, "replyTreeSize"=>0, "mostRecentActivity"=>"2014-05-11T01:18:14Z", "replies"=>[]}

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• {"id"=>"428542893913948160", "text"=>"Importance of #tumor #heterogeneity for #pgx #PLOSONE: Tumor-Specific Chr. Mis-Segregation Controls #Cancer... http://t.co/Mhswt0B3Rl", "created_at"=>"2014-01-29T14:59:22Z", "user"=>"Cancer_pgx", "user_name"=>"J. Todd Auman", "user_profile_image"=>"http://abs.twimg.com/sticky/default_profile_images/default_profile_3_normal.png"}

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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/1295082"], "description"=>"<p><b>A</b>, real-time qRT-PCR (right) quantification of the expression of genes associated with neural stem cell features as well as glioma cell migration and invasion, normalized to <i>ACTB</i>, in cells of the same cultures used for zymography, and enzyme immunometric assays (left) for quantification of VEGFA (VEGF-165) and SPP1 (Osteopontin) in conditioned medium, normalized by cell numbers. Bar and line height are mean and SD based on quantification of 3–6 sets of independent cultures. <b>B</b>, western blot (top) and immunocytofluorescence (bottom) of EGFR (1∶1000 from Cell Signaling) in U251 parental (P) and two clonal NS lines (NS1 and NS2). <b>C</b>, soft agar colony formation assay of U251 parental (P), NS1, and P-E1wd lines. <b>D</b>, s.c. tumorigenicity assay of cells described above, with follow-up of tumor growth as described previously <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080898#pone.0080898-Mayes1\" target=\"_blank\">[33]</a>. <b>E</b>, immunocytofluorescence analysis of NS1 and SA1-NS before and after being subjected to neural stem cell differentiation conditions described in Methods.</p>", "links"=>[], "tags"=>["phenotypes", "subpopulation", "cells"], "article_id"=>862029, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g004", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distinct_phenotypes_of_subpopulation_cells_in_U251_/862029", "title"=>"Distinct phenotypes of subpopulation cells in U251.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295100"], "description"=>"<p>FISH was performed 1 day after exposure to γ-rays, in single dose, at the 2 and 5 Gy levels.</p>", "links"=>[], "tags"=>[], "article_id"=>862043, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g008", "stats"=>{"downloads"=>2, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_radiation_on_Chr7_MS_/862043", "title"=>"Effect of radiation on Chr7-MS.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295081"], "description"=>"<p><b>A</b>, representative metaphase FISH pictures of PTEN/CEP10 and EGFR/CEP7 dual probes showing all cells carrying one copy of Chr10, an unknown chromosome with a PTEN translocation, and three cell types differing in their composition of normal and derivative Chr7 (dChr7). <i>Arrow</i> points to dChr7; <i>arrowhead</i> to normal Chr7. <b>B</b>, percentage of majority cells in the parental culture, derived or converted SA or NS subcultures, and the parental culture after lentiviral transductions by pTRIPZ-Vec (P-Vec), pTRIPZ-EFEMP1 with (P-E1) or after withdrawal (P-E1wd) of doxycyclin. <b>C–D</b>, comparison of DNA copy number variation in chromosomes 7, 8, 17, and 22 for U251 parental derived or converted NS subcultures of NS1 or SA1-NS, respectively. The Y axis is the log ratio of intensity (the ratio of test sample and normal blood) from comparative genome hybridization. Amplifications or deletions are shown by blue lines above or below the red or green areas, respectively, based on Z-score, and those with marked changes are highlighted in purple.</p>", "links"=>[], "tags"=>["karyotypes", "subpopulation", "cells"], "article_id"=>862028, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g003", "stats"=>{"downloads"=>0, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distinct_karyotypes_of_three_subpopulation_cells_in_U251_/862028", "title"=>"Distinct karyotypes of three subpopulation cells in U251.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295099"], "description"=>"<p>U251-NS1 infected with retrovirual vectors of VEGF-165 and LacZ described previously <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080898#pone.0080898-Hu1\" target=\"_blank\">[35]</a> were s.c. implanted in nude mice as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080898#s2\" target=\"_blank\">Materials and Methods</a>.</p>", "links"=>[], "tags"=>["u251-ns1", "tumorigenicity", "overexpression"], "article_id"=>862042, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g007", "stats"=>{"downloads"=>2, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Re_examination_of_U251_NS1_cell_s_c_tumorigenicity_after_overexpression_of_VEGFA_/862042", "title"=>"Re-examination of U251-NS1 cell s.c. tumorigenicity after overexpression of VEGFA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295072"], "description"=>"<p><b>A</b>, ternary plot of population proportions with 1 copy (deletion), 2 copies (normal), and 3 or more (amplification) copies of Chr7, based on CEP7 signals in Ffuorescent <i>in situ</i> hybridization (FISH) of 14 glioblastoma multiformes (GBMs, <i>Red triangle</i>) and 12 oligodendroglial tumor (OGs, <i>black square</i>), <i>P</i> = 0.0012 from MANOVA analysis. <b>B</b>, comparison of tumor heterogeneity with regard to chromosome 7 (Chr7) aneuploidy in the original tumor (T) and corresponding 3–4 week-old primary cultures under serum adherent (SA) or neural sphere (NS) culture conditions. <b>C–D</b>, patterns of cells with Chr7-CNV in sequential and high-EGFR amplified GBMs. Representative FISH pictures of cells carrying 1–4 copies of Chr7 with focal EGFR amplification.</p>", "links"=>[], "tags"=>["cells", "chr7", "high-", "low-grade", "gliomas", "glioma"], "article_id"=>862019, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g001", "stats"=>{"downloads"=>1, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proportion_of_cells_with_Chr7_number_variation_in_high_and_low_grade_gliomas_and_glioma_primary_cultures_/862019", "title"=>"Proportion of cells with Chr7 number variation in high- and low-grade gliomas and glioma primary cultures.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295102", "https://ndownloader.figshare.com/files/1295103", "https://ndownloader.figshare.com/files/1295104"], "description"=>"<div><p>Aneuploidy with chromosome instability is a cancer hallmark. We studied chromosome 7 (Chr7) copy number variation (CNV) in gliomas and in primary cultures derived from them. We found tumor heterogeneity with cells having Chr7-CNV commonly occurs in gliomas, with a higher percentage of cells in high-grade gliomas carrying more than 2 copies of Chr7, as compared to low-grade gliomas. Interestingly, all Chr7-aneuploid cell types in the parental culture of established glioma cell lines reappeared in single-cell-derived subcultures. We then characterized the biology of three syngeneic glioma cultures dominated by different Chr7-aneuploid cell types. We found phenotypic divergence for cells following Chr7 mis-segregation, which benefited overall tumor growth <i>in vitro</i> and <i>in vivo</i>. Mathematical modeling suggested the involvement of chromosome instability and interactions among cell subpopulations in restoring the optimal equilibrium of tumor cell types. Both our experimental data and mathematical modeling demonstrated that the complexity of tumor heterogeneity could be enhanced by the existence of chromosomes with structural abnormality, in addition to their mis-segregations. Overall, our findings show, for the first time, the involvement of chromosome instability in maintaining tumor heterogeneity, which underlies the enhanced growth, persistence and treatment resistance of cancers.</p></div>", "links"=>[], "tags"=>["chromosome", "mis-segregation", "controls", "cancer", "plasticity", "maintaining"], "article_id"=>862045, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0080898.s001", "https://dx.doi.org/10.1371/journal.pone.0080898.s002", "https://dx.doi.org/10.1371/journal.pone.0080898.s003"], "stats"=>{"downloads"=>11, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Tumor_Specific_Chromosome_Mis_Segregation_Controls_Cancer_Plasticity_by_Maintaining_Tumor_Heterogeneity_/862045", "title"=>"Tumor-Specific Chromosome Mis-Segregation Controls Cancer Plasticity by Maintaining Tumor Heterogeneity", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295101"], "description"=>"<p>The parameter r₂₀ is chosen to have an initial doubling time of 1.1 days.</p>", "links"=>[], "tags"=>[], "article_id"=>862044, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.t001", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Parameters_that_best_fit_of_the_percentage_time_course_data_/862044", "title"=>"Parameters that best fit of the percentage time course data.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295098"], "description"=>"<p><b>A</b>, schematic illustration of the working mechanism with Chr7-MS resulting in heterogeneous subpopulations and cellular phenotype inter-conversion, with STIC inhibiting growth of TMC and/or TMC stimulating growth of STIC. The Chr7 composition was shown for representative subpopulation cells in U251, based on metaphase FISH.A higher growth rate for TMC (<i>r</i>₃) was shown compared to that for STIC (<i>r</i>₂). Cells marked by black circles were seen in metaphase FISH analysis, suggesting their ability to grow <i>in vitro</i>. Cells marked by gray boxes were not seen in metaphase FISH analysis, suggesting they are unable, or have very low ability to grow <i>in vitro</i>. <b>B</b>, changes in population equilibrium from homogeneity to heterogeneity in NS1 after serial, three-day passages in SA-culture conditions, using the same cell plating density (5×10⁵/100 mm dish), with cell types determined by FISH (experimental) and then modeled using different parameters as detailed in Methods. <b>C</b>. changes in cell growth speed as measured or predicted by various mathematical models. <b>D</b>, s.c. tumorigenicity assay showing the TMC features of 3Chr7:2n,1d cells converted from STIC by increasing percentage tumor onset (a tumor size of ∼50 mm³). The plot with percent uses percents computed via the Kaplan-Meier method. Log-rank test for trend with 3Chr7:2n,1d cells shows two-sided <i>P</i><0.0001.</p>", "links"=>[], "tags"=>["modeling", "equilibrium", "homogeneity"], "article_id"=>862041, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g006", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mathematical_modeling_of_experimental_data_with_changes_in_population_equilibrium_from_homogeneity_to_heterogeneity_/862041", "title"=>"Mathematical modeling of experimental data with changes in population equilibrium from homogeneity to heterogeneity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295097"], "description"=>"<p><b>A</b>, H&E images (2X) and FISH images (100X) of i.c. xenografts derived from NS1. <i>Arrowhead</i>, <i>double</i> and <i>single arrow</i> point to cells with 1, 2, and 3 copies of Chr7, respectively. <b>B</b>, comparison of cell population equilibrium in NS1 (<i>in vitro</i> culture) and the derived i.c. tumors. <b>C</b>, fluorescence images of i.c. xenografts derived from co-implantation of RFP-labeled STIC-enriched U251-NS and GFP-labeled U251 in a 9∶1 ratio. <b>D–E</b>, immunofluorescence images of i.c. xenografts from co-implantation of the two lines in a 1∶99 ratio, with purple color marking BMI1 and MELK expression by RFP cells at the tumor boundary and their expression of CD133 and SPARC in vascular mimicry within the bulk tumor mass. <b>F</b>, confocal immunofluorescence images of i.c. xenografts derived from 100% RFP cells, with yellow color marking co-localization of RFP with CD31 or GFAP. <b>G</b>, Kaplan-Meier survival curves of mice after implanting a mixture of U251 parental and STIC-enriched NS subculture. Adjusted Hazard ratios (HRs) from the stratified analysis and p-value are from Cox regression analyses examining the effect of STIC percentage on survival.</p>", "links"=>[], "tags"=>["subpopulations", "u251", "optimal", "equilibrium", "benefiting"], "article_id"=>862040, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g005", "stats"=>{"downloads"=>2, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distinct_8220_go_8221_and_8220_grow_8221_features_of_two_cell_subpopulations_in_U251_with_optimal_equilibrium_benefiting_overall_tumor_growth_/862040", "title"=>"Distinct “go” and “grow” features of two cell subpopulations in U251 with optimal equilibrium benefiting overall tumor growth.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}
• {"files"=>["https://ndownloader.figshare.com/files/1295074"], "description"=>"<p><b>A–B</b>, percentage of cells with Chr7-CNV in glioma cell lines, and their SA and NS subcultures from single (e.g. 1, 2) or mixed (mix) soft-agar colonies, respectively. Whole chromosome numbers (WCN) ranging near the mode were found in >50% of cells in each glioma cell line. <b>D</b>, FISH pictures showing normal (n) and derivative (d) Chr7 and the unknown (?) chromosome carrying a translocated EGFR from a representative metaphase cell for each cell line. The chromosome is shown by DAPI (blue), centromere and EGFR are shown by FISH probes for CEP7 (green) and EGFR (red).</p>", "links"=>[], "tags"=>["heterogeneity", "cells", "chr7-cnv", "established", "glioma", "lines", "clonal"], "article_id"=>862021, "categories"=>["Biological Sciences"], "users"=>["Yuanjie Hu", "Ning Ru", "Huasheng Xiao", "Abhishek Chaturbedi", "Neil T. Hoa", "Xiao-Jun Tian", "Hang Zhang", "Chao Ke", "Fengrong Yan", "Jodi Nelson", "Zhenzhi Li", "Robert Gramer", "Liping Yu", "Eric Siegel", "Xiaona Zhang", "Zhenyu Jia", "Martin R. Jadus", "Charles L. Limoli", "Mark E. Linskey", "Jianhua Xing", "Yi-Hong Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080898.g002", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Equilibrium_of_heterogeneity_in_cells_with_Chr7_CNV_in_established_glioma_cell_lines_and_their_clonal_subcultures_/862021", "title"=>"Equilibrium of heterogeneity in cells with Chr7-CNV in established glioma cell lines and their clonal subcultures.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-25 03:51:08"}

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Relative Metric

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F1000Prime