Hybridization-Based Detection of Helicobacter pylori at Human Body Temperature Using Advanced Locked Nucleic Acid (LNA) Probes
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Scopus | Further Information

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Figshare

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  • {"files"=>["https://ndownloader.figshare.com/files/1293178"], "description"=>"<p>(A) Detection of <i>H. pylori</i> using the HP_LNA/2OMe_PS probe in a histological slide of a gastric biopsy specimen of an infected patient; (B) Experiment control performed in parallel using the HP_LNA/2OMe_PS probe in a histological slide of a gastric biopsy specimen of an non infected patient. Arrows indicate the presence of <i>H. pylori</i> infecting the gastric mucosa. All images were taken at equal exposure times Scale bars: 10 µm. Original magnification of ×1000.</p>", "links"=>[], "tags"=>["detection", "paraffin-embedded", "sections", "gastric"], "article_id"=>860469, "categories"=>["Biological Sciences"], "users"=>["Sílvia Fontenete", "Nuno Guimarães", "Marina Leite", "Ceu Figueiredo", "Jesper Wengel", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081230.g004", "stats"=>{"downloads"=>5, "page_views"=>165, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FISH_detection_of_H_pylori_in_paraffin_embedded_sections_of_gastric_biopsies_using_2_O_methyl_LNA_FISH_detection_conditions_/860469", "title"=>"FISH detection of <i>H.pylori</i> in paraffin-embedded sections of gastric biopsies, using 2’-O-methyl/LNA FISH detection conditions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-22 02:43:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/1293179"], "description"=>"<div><p>The understanding of the human microbiome and its influence upon human life has long been a subject of study. Hence, methods that allow the direct detection and visualization of microorganisms and microbial consortia (e.g. biofilms) within the human body would be invaluable. In here, we assessed the possibility of developing a variant of fluorescence in situ hybridization (FISH), named fluorescence in vivo hybridization (FIVH), for the detection of <i>Helicobacter pylori</i>. Using oligonucleotide variations comprising locked nucleic acids (LNA) and 2’-O-methyl RNAs (2’OMe) with two types of backbone linkages (phosphate or phosphorothioate), we were able to successfully identify two probes that hybridize at 37 °C with high specificity and sensitivity for <i>H. pylori</i>, both in pure cultures and in gastric biopsies. Furthermore, the use of this type of probes implied that toxic compounds typically used in FISH were either found to be unnecessary or could be replaced by a non-toxic substitute. We show here for the first time that the use of advanced LNA probes in FIVH conditions provides an accurate, simple and fast method for <i>H. pylori</i> detection and location, which could be used in the future for potential <i>in vivo</i> applications either for this microorganism or for others.</p> </div>", "links"=>[], "tags"=>["hybridization-based", "detection", "locked", "nucleic", "probes"], "article_id"=>860470, "categories"=>["Biological Sciences"], "users"=>["Sílvia Fontenete", "Nuno Guimarães", "Marina Leite", "Ceu Figueiredo", "Jesper Wengel", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081230", "stats"=>{"downloads"=>10, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Hybridization_Based_Detection_of_Helicobacter_pylori_at_Human_Body_Temperature_Using_Advanced_Locked_Nucleic_Acid_LNA_Probes/860470", "title"=>"Hybridization-Based Detection of <i>Helicobacter pylori</i> at Human Body Temperature Using Advanced Locked Nucleic Acid (LNA) Probes", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-11-22 02:43:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/1293172"], "description"=>"<p>Structures of LNA and 2’ O-methyl RNA monomers (phosphate and phosphorothioate structures) used.</p>", "links"=>[], "tags"=>["lna", "o-methyl", "rna", "monomers", "phosphorothioate"], "article_id"=>860463, "categories"=>["Biological Sciences"], "users"=>["Sílvia Fontenete", "Nuno Guimarães", "Marina Leite", "Ceu Figueiredo", "Jesper Wengel", "Nuno Filipe Azevedo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081230.g001", "stats"=>{"downloads"=>7, "page_views"=>212, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Structures_of_LNA_and_2_8217_O_methyl_RNA_monomers_phosphate_and_phosphorothioate_structures_used_/860463", "title"=>"Structures of LNA and 2’ O-methyl RNA monomers (phosphate and phosphorothioate structures) used.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-22 02:43:30"}

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Relative Metric

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