R-Loops in Proliferating Cells but Not in the Brain: Implications for AOA2 and Other Autosomal Recessive Ataxias
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"R-Loops in proliferating cells but not in the brain: Implications for AOA2 and other autosomal recessive ataxias", "type"=>"journal", "authors"=>[{"first_name"=>"Abrey J.", "last_name"=>"Yeo", "scopus_author_id"=>"55969277200"}, {"first_name"=>"Olivier J.", "last_name"=>"Becherel", "scopus_author_id"=>"6602583654"}, {"first_name"=>"John E.", "last_name"=>"Luff", "scopus_author_id"=>"12780815500"}, {"first_name"=>"Jason K.", "last_name"=>"Cullen", "scopus_author_id"=>"55523103700"}, {"first_name"=>"Thidathip", "last_name"=>"Wongsurawat", "scopus_author_id"=>"8553139800"}, {"first_name"=>"Piroon", "last_name"=>"Jenjaroenpoon", "scopus_author_id"=>"56114206400"}, {"first_name"=>"Vladimir A.", "last_name"=>"Kuznetsov", "scopus_author_id"=>"7404320226"}, {"first_name"=>"Peter J.", "last_name"=>"McKinnon", "scopus_author_id"=>"57193378843"}, {"first_name"=>"Martin F.", "last_name"=>"Lavin", "scopus_author_id"=>"35427118100"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24637776", "doi"=>"10.1371/journal.pone.0090219", "sgr"=>"84898618015", "scopus"=>"2-s2.0-84898618015", "issn"=>"19326203", "pui"=>"372838524"}, "id"=>"93972677-5fa6-3f05-b4aa-754542970e57", "abstract"=>"Disruption of the Setx gene, defective in ataxia oculomotor apraxia type 2 (AOA2) leads to the accumulation of DNA/RNA hybrids (R-loops), failure of meiotic recombination and infertility in mice. We report here the presence of R-loops in the testes from other autosomal recessive ataxia mouse models, which correlate with fertility in these disorders. R-loops were coincident in cells showing high basal levels of DNA double strand breaks and in those cells undergoing apoptosis. Depletion of Setx led to high basal levels of R-loops and these were enhanced further by DNA damage both in vitro and in vivo in tissues with proliferating cells. There was no evidence for accumulation of R-loops in the brains of mice where Setx, Atm, Tdp1 or Aptx genes were disrupted. These data provide further evidence for genome destabilization as a consequence of disrupted transcription in the presence of DNA double strand breaks arising during DNA replication or recombination. They also suggest that R-loop accumulation does not contribute to the neurodegenerative phenotype in these autosomal recessive ataxias.", "link"=>"http://www.mendeley.com/research/rloops-proliferating-cells-not-brain-implications-aoa2-other-autosomal-recessive-ataxias", "reader_count"=>17, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>6, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>10, "Medicine and Dentistry"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"United Kingdom"=>1, "Israel"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84898618015"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84898618015?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84898618015&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84898618015&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84898618015", "dc:identifier"=>"SCOPUS_ID:84898618015", "eid"=>"2-s2.0-84898618015", "dc:title"=>"R-Loops in proliferating cells but not in the brain: Implications for AOA2 and other autosomal recessive ataxias", "dc:creator"=>"Yeo A.J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-03-17", "prism:coverDisplayDate"=>"17 March 2014", "prism:doi"=>"10.1371/journal.pone.0090219", "citedby-count"=>"29", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"The University of Queensland", "affiliation-city"=>"Brisbane", "affiliation-country"=>"Australia"}, {"@_fa"=>"true", "affilname"=>"QIMR Berghofer Medical Research Institute", "affiliation-city"=>"Brisbane", "affiliation-country"=>"Australia"}], "pubmed-id"=>"24637776", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e90219", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0090219", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Twitter

Counter

  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"64", "xml_views"=>"7", "html_views"=>"213"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"38", "xml_views"=>"5", "html_views"=>"111"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"36", "xml_views"=>"0", "html_views"=>"117"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"35", "xml_views"=>"0", "html_views"=>"118"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"121"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"131"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"153"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"2", "html_views"=>"143"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"102"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"90"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"100"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"105"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"95"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"106"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"82"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"74"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"102"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"88"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"127"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"105"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"63"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"100"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"56"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"49", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"3", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"39"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"51", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"17"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1422712"], "description"=>"<p>Model depicting the requirements for R-loops formation in the various ARCAs model. While transcription is implied in the formation of R-loops, active replication appears to be a necessary condition too. Thus R-loops accumulation does not appear to contribute to the neurodegenerative phenotype observed in autosomal recessive cerebellar ataxias.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "preferentially", "cycling", "post-mitotic"], "article_id"=>963885, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g011", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_R_loops_preferentially_form_in_cycling_but_not_in_post_mitotic_cells_/963885", "title"=>"R-loops preferentially form in cycling but not in post-mitotic cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422697"], "description"=>"<p><b>A.</b> Whole brain sections from wildtype (<i>Setx<sup>+/+</sup></i>) and <i>Setx<sup>−/−</sup></i> were stained for R-loop (Red) and apoptosis (TUNEL, Green). Nuclei were labelled with DAPI. <b>B.</b> Wildtype and <i>Setx<sup>−/−</sup></i> cerebellar sections staining with R-loops and TUNEL. DAPI stained nuclei. <b>C.</b> As a control for R-loop and TUNEL staining, both wildtype and <i>Setx<sup>−/−</sup></i> testes sections are shown. Scale bar, 100 µm.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "r-loops"], "article_id"=>963870, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g003", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Absence_of_R_loops_in_Setx_8722_8722_nervous_tissues_/963870", "title"=>"Absence of R-loops in <i>Setx<sup>−/−</sup></i> nervous tissues.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422711"], "description"=>"<p>Graphs show the percentage of apoptotic (TUNEL) cells only, cells containing R-loops only and apoptotic cells containing R-loops in the different tissues of <i>Setx</i> (<b>A</b> and <b>B</b>) and <i>Tdp1</i> mice (<b>C</b>). Counts were performed using 5 fields of view per tissue per animal (n = 3). Higher levels of these three cell types were observed in TPT-treated knockout animals.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "r-loop", "tpt-treated", "knockout"], "article_id"=>963884, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g010", "stats"=>{"downloads"=>1, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Increased_R_loop_formation_in_TPT_treated_knockout_Setx_and_Tdp1_mice_/963884", "title"=>"Increased R-loop formation in TPT-treated knockout <i>Setx</i> and <i>Tdp1</i> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422708"], "description"=>"<p>Wildtype and <i>Setx<sup>−/−</sup></i>histological sections of intestine and spleen, two tissues with a high proliferative capacity, were stained for R-loops, TUNEL, and Ki67. TPT induced the formation of R-loops and apoptosis in both intestine and spleen. TPT disrupted the structure of the small intestine in <i>Setx<sup>−/−</sup></i> animals supporting the severe weight loss observed in these mice. R-loop (red), TUNEL (green) and Ki67 (red). DAPI (blue) stained nuclei. Scale bar, 100 µm.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "r-loops", "proliferating", "cells", "topotecan"], "article_id"=>963881, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g008", "stats"=>{"downloads"=>1, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Induction_of_R_loops_in_proliferating_cells_after_topotecan_treatment_/963881", "title"=>"Induction of R-loops in proliferating cells after topotecan treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422688"], "description"=>"<p><b>A.</b> Histological cross-sections of seminiferous tubules from ARCA mouse models <i>Setx<sup>−/−</sup></i>, <i>Atm<sup>−/−</sup></i>, <i>Aptx<sup>−/−</sup></i>, <i>Tdp1<sup>−/−</sup></i> and wildtype (WT) littermates. Sections were H & E stained. Scale bar, 100 µm. <b>B.</b> Immunostaining of testes sections with R-loop (S9.6) antibody (red) and TUNEL (green). Nuclei were stained using Hoechst 33342 (blue). Scale bar, 100 µm. Magnified views of the tubules are also shown. Representative spermatocytes staining positive for both R-loop and TUNEL are indicated by white arrows. <b>C.</b> Immunostaining of testes sections with γH2AX (red), a marker of DNA DSBs, and TUNEL (green). Scale bar, 100 µm.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "hybrids", "accumulation", "spermatocytes", "arca"], "article_id"=>963861, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g001", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evidence_of_DNA_RNA_hybrids_R_loops_accumulation_in_spermatocytes_of_ARCA_mouse_model_/963861", "title"=>"Evidence of DNA/RNA hybrids (R-loops) accumulation in spermatocytes of ARCA mouse model.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422701"], "description"=>"<p>Wiltype (WT), <i>Setx<sup>−/−</sup></i> and <i>Tdp1<sup>−/−</sup></i> mice were treated with a daily dose of topotecan (TPT, 2 mg/kg/day) over a period of 9 days in order to exacerbate the formation of R-loops in these mice. Mice of each genotype were injected daily with a dose of TPT (2 mg/kg/day) and weights were recorded daily (n = 3). Controls were injected with an equivalent volume of purified water.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "mice", "topotecan"], "article_id"=>963874, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g006", "stats"=>{"downloads"=>2, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Treatment_of_Setx_and_Tdp1_mice_with_Topotecan_induces_severe_weight_loss_/963874", "title"=>"Treatment of <i>Setx</i> and <i>Tdp1</i> mice with Topotecan induces severe weight loss.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422713", "https://ndownloader.figshare.com/files/1422714", "https://ndownloader.figshare.com/files/1422715", "https://ndownloader.figshare.com/files/1422716", "https://ndownloader.figshare.com/files/1422717"], "description"=>"<div><p>Disruption of the <i>Setx</i> gene, defective in ataxia oculomotor apraxia type 2 (AOA2) leads to the accumulation of DNA/RNA hybrids (R-loops), failure of meiotic recombination and infertility in mice. We report here the presence of R-loops in the testes from other autosomal recessive ataxia mouse models, which correlate with fertility in these disorders. R-loops were coincident in cells showing high basal levels of DNA double strand breaks and in those cells undergoing apoptosis. Depletion of <i>Setx</i> led to high basal levels of R-loops and these were enhanced further by DNA damage both <i>in vitro</i> and <i>in vivo</i> in tissues with proliferating cells. There was no evidence for accumulation of R-loops in the brains of mice where <i>Setx</i>, <i>Atm</i>, <i>Tdp1</i> or <i>Aptx</i> genes were disrupted. These data provide further evidence for genome destabilization as a consequence of disrupted transcription in the presence of DNA double strand breaks arising during DNA replication or recombination. They also suggest that R-loop accumulation does not contribute to the neurodegenerative phenotype in these autosomal recessive ataxias.</p></div>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "proliferating", "cells", "implications", "aoa2", "autosomal", "recessive"], "article_id"=>963886, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0090219.s001", "https://dx.doi.org/10.1371/journal.pone.0090219.s002", "https://dx.doi.org/10.1371/journal.pone.0090219.s003", "https://dx.doi.org/10.1371/journal.pone.0090219.s004", "https://dx.doi.org/10.1371/journal.pone.0090219.s005"], "stats"=>{"downloads"=>1, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_R_Loops_in_Proliferating_Cells_but_Not_in_the_Brain_Implications_for_AOA2_and_Other_Autosomal_Recessive_Ataxias_/963886", "title"=>"R-Loops in Proliferating Cells but Not in the Brain: Implications for AOA2 and Other Autosomal Recessive Ataxias", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422710"], "description"=>"<p>Histological sections from <i>Tdp1<sup>−/−</sup></i> mice were stained for R-loops, TUNEL and Ki67 after topotecan exposure. Similar to <i>Setx<sup>−/−</sup></i>, TPT induced the formation of R-loops formation only in proliferative tissues testes, intestine and spleen). No R-loops were detected in post-mitotic tissues (brain and cerebellum). Scale bar, 100 µm.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "r-loops", "proliferative", "tissues", "topotecan"], "article_id"=>963883, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g009", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Induction_of_R_loops_only_in_proliferative_tissues_in_Tdp1_8722_8722_after_topotecan_treatment_/963883", "title"=>"Induction of R-loops only in proliferative tissues in <i>Tdp1<sup>−/−</sup></i> after topotecan treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422691"], "description"=>"<p><b>A.</b> Percentage of seminiferous tubules containing R-loops. <b>B.</b> Average number of apoptotic spermatocytes per tubule in testes from ARCA mice. <b>C.</b> Percentage of R-loop & TUNEL-positive cells. <b>D.</b> Average number of apoptotic cells containing DNA DSBs (γH2AX). For each panel, 200 tubules were examined per animal and cells were counted in each tubule (n = 3). Error bars represent SEM.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "dna", "quantitation", "arcas"], "article_id"=>963864, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g002", "stats"=>{"downloads"=>3, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_R_loop_formation_apoptosis_and_DNA_damage_quantitation_in_ARCAs_mouse_testes_/963864", "title"=>"R-loop formation, apoptosis, and DNA damage quantitation in ARCAs mouse testes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422704"], "description"=>"<p>Histological sections of brain, cerebellum, and testes were immunostained for R-loops (Red) and TUNEL (Green). No R-loops were detected in brain and cerebellum sections after TPT treatment. In contrast, an increase in the number of R-loop-containing cells was observed in wildtype and <i>Setx<sup>−/−</sup></i> after TPT exposure suggesting that R-loop preferentially form in proliferating cells, most likely due to collision between the DNA replication machinery and the transcription apparatus. DAPI stained nuclei and Ki67 (Red) was used as a marker for proliferation. Scale bar, 100 µm.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "induce", "r-loops", "post-mitotic", "tissues"], "article_id"=>963877, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g007", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Topotecan_treatment_does_not_induce_R_loops_formation_in_post_mitotic_nervous_tissues_in_Setx_8722_8722_mice_/963877", "title"=>"Topotecan treatment does not induce R-loops formation in post-mitotic nervous tissues in <i>Setx<sup>−/−</sup></i> mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422700"], "description"=>"<p><b>A.</b> Knock down of senataxin in HeLa cells using short interfering RNA (siRNA) induced the accumulation of R-loops in nucleoli. Knock down efficiency for senataxin is shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090219#pone.0090219.s002\" target=\"_blank\">Figure S2</a>. Treatment of these cells with 25 µM of camptothecin (CPT) led to the accumulation of R-loops in control cells (Ctrl siRNA) and a further increase in senataxin knockdown cells (<i>SETX</i> siRNA). Pre-treatment of these with RNA polymerase inhibitor Actinomycin D (AD, 5 µg/ml), ablated the CPT-induced formation of R-loop in both cell types. <b>B.</b> Quantitation of R-loops formation in control and senataxin knockdown cells. No R-loops were detected in ctrl siRNA-treated cells under normal growing conditions. CPT induced the formation of R-loops in both cell types and pre-treatment these cells with AD prevented the induction of R-loops following DNA damage exposure. Levels of R-loops returned to basal levels in <i>SETX</i> siRNA-treated cells after addition of AD.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "r-loop", "dna"], "article_id"=>963873, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g005", "stats"=>{"downloads"=>0, "page_views"=>33, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Induction_of_R_loop_after_DNA_damage_exposure_/963873", "title"=>"Induction of R-loop after DNA damage exposure.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1422698"], "description"=>"<p>UCSC Genome browser shows chromosome mapping of RLFS and poly(A) signal location at 3′ UTR of studied genes. RFLS (red box), and PCR products (black vertical lines connected by horizontal lines) are indicated on the diagram. <b>A.</b> RLFS prediction in the 3′ UTR of the mouse <i>Pkd2l1</i> gene located in the HS44.2 locus on chromosome 19. See <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090219#pone.0090219.s005\" target=\"_blank\">Figure S5</a> for sequence details. DRIP quantitation from <i>Setx<sup>+/+</sup></i> and <i>Setx<sup>−/−</sup></i> showed similar background levels of R-loops in brain and cerebellum. Only a significant increase in R-loop formation was observed in <i>Setx<sup>−/−</sup></i>testes as compared to <i>Setx<sup>+/+</sup></i>(Students t-test, p<0.05, n = 3). <b>B</b>. RLFS prediction in the 3′UTR of the mouse <i>Foxo4</i> gene located on the X chromosome. DRIP quantitation from <i>Setx<sup>+/+</sup></i> and <i>Setx<sup>−/−</sup></i> showed similar background levels of R-loops in brain and cerebellum. Similarly, a significant increase in R-loop formation was only observed in <i>Setx<sup>−/−</sup></i>testes (Students t-test, p<0.05, n = 3). <b>C.</b> Treatment of <i>Setx<sup>−/−</sup></i> DRIP samples with S1 Nuclease and RNAse H confirmed the specificity of the S9.6 (R-loop) antibody towards DNA/RNA hybrids as shown by reduced fluorescence intensities on Bioanalyzer Spectra.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "cell biology", "Molecular cell biology", "genetics", "Genetic dominance", "Autosomal recessive traits", "cytogenetics", "Genetics of disease", "Clinical genetics", "neurology", "Neurodegenerative diseases", "Motor neuron diseases", "Model organisms", "Animal models", "Mouse models", "sequences", "r-loops", "detection", "drip"], "article_id"=>963871, "categories"=>["Biological Sciences"], "users"=>["Abrey J. Yeo", "Olivier J. Becherel", "John E. Luff", "Jason K. Cullen", "Thidathip Wongsurawat", "Piroon Jenjaroenpoon", "Vladimir A. Kuznetsov", "Peter J. McKinnon", "Martin F. Lavin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090219.g004", "stats"=>{"downloads"=>1, "page_views"=>61, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_R_loops_forming_sequences_RLFS_prediction_and_R_loops_detection_using_DRIP_assay_/963871", "title"=>"R-loops forming sequences (RLFS) prediction and R-loops detection using DRIP assay.)", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 02:57:27"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"24", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"6", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"39", "full-text"=>"36", "pdf"=>"14", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"26", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"24", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"22", "full-text"=>"31", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"22", "full-text"=>"29", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"28", "full-text"=>"21", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"28", "full-text"=>"27", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"30", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"11", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"33", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"25", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"17", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"20", "full-text"=>"13", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"27", "full-text"=>"30", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"36", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"30", "full-text"=>"39", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"24", "full-text"=>"24", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"29", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"23", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"30", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"25", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"30", "full-text"=>"29", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"21", "full-text"=>"31", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"27", "full-text"=>"30", "pdf"=>"28", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"28", "full-text"=>"28", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"18", "full-text"=>"30", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"28", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"35", "full-text"=>"40", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"28", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"18", "full-text"=>"10", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"22", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"23", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[266]}]}
Loading … Spinner
There are currently no alerts