Para-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom Agaricus bisporus
Publication Date
March 10, 2014
Journal
PLOS ONE
Authors
Zhonglei Lu, Xiangxiang Kong, Zhaoming Lu, Meixiang Xiao, et al
Volume
9
Issue
3
Pages
e91298
DOI
https://dx.plos.org/10.1371/journal.pone.0091298
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0091298
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24614118
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948851
Europe PMC
http://europepmc.org/abstract/MED/24614118
Web of Science
000332839300107
Scopus
84897539258
Mendeley
http://www.mendeley.com/research/paraaminobenzoic-acid-paba-synthase-enhances-thermotolerance-mushroom-agaricus-bisporus
Events
Loading … Spinner
Mendeley 1329 Apr 12:11 UTC

Mendeley | Further Information

{"title"=>"Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom agaricus bisporus", "type"=>"journal", "authors"=>[{"first_name"=>"Zhonglei", "last_name"=>"Lu", "scopus_author_id"=>"55286301700"}, {"first_name"=>"Xiangxiang", "last_name"=>"Kong", "scopus_author_id"=>"55336509300"}, {"first_name"=>"Zhaoming", "last_name"=>"Lu", "scopus_author_id"=>"56096013400"}, {"first_name"=>"Meixiang", "last_name"=>"Xiao", "scopus_author_id"=>"56096896400"}, {"first_name"=>"Meiyuan", "last_name"=>"Chen", "scopus_author_id"=>"56097085700"}, {"first_name"=>"Liang", "last_name"=>"Zhu", "scopus_author_id"=>"7404201017"}, {"first_name"=>"Yuemao", "last_name"=>"Shen", "scopus_author_id"=>"7404766522"}, {"first_name"=>"Xiangyang", "last_name"=>"Hu", "scopus_author_id"=>"8051753000"}, {"first_name"=>"Siyang", "last_name"=>"Song", "scopus_author_id"=>"7403349884"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"372755294", "sgr"=>"84897539258", "issn"=>"19326203", "pmid"=>"24614118", "scopus"=>"2-s2.0-84897539258", "doi"=>"10.1371/journal.pone.0091298", "isbn"=>"10.1371/journal.pone.0091298"}, "id"=>"1b0c88ff-4f51-399f-8f44-ae21308dddb2", "abstract"=>"Most mushrooms are thermo-sensitive to temperatures over 23°C, which greatly restricts their agricultural cultivation. Understanding mushroom's innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. Agaricus bisporus strain 02 is a relatively thermotolerant mushroom strain, while strain 8213 is quite thermo-sensitive. Here, we compared their responses at proteomic level to heat treatment at 33°C. We identified 73 proteins that are differentially expressed between 02 and 8213 or induced upon heat stress in strain 02 itself, 48 of which with a known identity. Among them, 4 proteins are constitutively more highly expressed in 02 than 8213; and they can be further upregulated in response to heat stress in 02, but not in 8213. One protein is encoded by the para-aminobenzoic acid (PABA) synthase gene Pabs, which has been shown to scavenge the reactive oxygen species in vitro. Pabs mRNA and its chemical product PABA show similar heat stress induction pattern as PABA synthase protein and are more abundant in 02, indicating transcriptional level upregulation of Pabs upon heat stress. A specific inhibitor of PABA synthesis impaired thermotolerance of 02, while exogenous PABA or transgenic overexpression of 02 derived PABA synthase enhanced thermotolerance of 8213. Furthermore, compared to 8213, 02 accumulated less H2O2 but more defense-related proteins (e.g., HSPs and Chitinase) under heat stress. Together, these results demonstrate a role of PABA in enhancing mushroom thermotolerance by removing H2O2 and elevating defense-related proteins.", "link"=>"http://www.mendeley.com/research/paraaminobenzoic-acid-paba-synthase-enhances-thermotolerance-mushroom-agaricus-bisporus", "reader_count"=>13, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>8, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>8}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>0}
Scopus 2014 Feb 22:10 UTC

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84897539258"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84897539258?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84897539258&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84897539258&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84897539258", "dc:identifier"=>"SCOPUS_ID:84897539258", "eid"=>"2-s2.0-84897539258", "dc:title"=>"Para-aminobenzoic acid (PABA) synthase enhances thermotolerance of mushroom agaricus bisporus", "dc:creator"=>"Lu Z.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-04-10", "prism:coverDisplayDate"=>"10 April 2014", "prism:doi"=>"10.1371/journal.pone.0091298", "citedby-count"=>"20", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Xiamen University", "affiliation-city"=>"Xiamen", "affiliation-country"=>"China"}], "pubmed-id"=>"24614118", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e91298", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}
Facebook10 Jul 01:23 UTC

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0091298", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}
Counter 351322 Feb 03:51 UTC

Counter

  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"28", "xml_views"=>"6", "html_views"=>"202"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"4", "html_views"=>"172"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"141"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"107"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"63"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"69"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"47"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"52"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"49"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"19"}
Figshare 17329 Apr 09:10 UTC

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1413567"], "description"=>"<p>Strains <i>02</i> and <i>8213</i> had been cultivated under the same regular condition until about 1 week before fruit body formation, each was then divided into two groups. One half was maintained at 18°C (control group); the other half was maintained at 30°C (high-temperature group) for 7 days. At Day 7, the fruit bodies were photographed (A), and the pileus diameter and biomass weight were recorded (B). Three independent replicates were performed. Data are expressed as average ± SEM. Unpaired t-tests were performed, ns: P>0.05, **: P<0.01.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "thermotolerant"], "article_id"=>956679, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g001", "stats"=>{"downloads"=>5, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mushroom_strain_02_is_more_thermotolerant_than_strain_8213_/956679", "title"=>"Mushroom strain <i>02</i> is more thermotolerant than strain <i>8213</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413570"], "description"=>"<p>(A) Representative 2-DE gels of mushrooms, identifying 73 proteins with a greater than 2-fold difference after high-temperature treatment (p<0.05). Molecular weight (MW) in kilodaltons and pI of proteins are indicated on the left and top of the gel, respectively. (B) Close-up view of some differentially expressed proteins spots. Three independent replicates were performed. <i>02</i>-NS: <i>02</i> non-stressed (23°C/24 h); <i>02</i>-HS: <i>02</i> heat-stressed (33°C/24 h); <i>8213</i>-NS: <i>8213</i> non-stressed (23°C/24 h); <i>8213</i>-HS: strain <i>8213</i> heat-stressed (33°C/24 h).</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "electrophoresis", "extracts"], "article_id"=>956682, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g002", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_2_D_electrophoresis_of_protein_extracts_from_02_and_8213_with_or_without_heat_stress_33_C_24_h_/956682", "title"=>"2-D electrophoresis of protein extracts from <i>02</i> and <i>8213</i> with or without heat stress (33°C/24 h).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413573"], "description"=>"<p>(A) Venn diagram showing 20 up-regulated and 5 down-regulated proteins among 48 identified proteins comparing <i>02</i>-HS with <i>02</i>-NS. Functional classification of those 25 heat stress induced proteins is shown on the bottom. (B) Heat-map is shown to indicate relative protein abundance among the 4 samples. The abundance of each protein spot in <i>02</i>-NS was given a reference value of 1.0, the abundance of that spot from <i>02</i>-HS, <i>8213</i>-NS and <i>8213</i>-HS were transformed into relative value. The different colors correspond to the values of protein level changes as indicated by the bar at the bottom of the heat map. Venn diagrams present the two-step filtering process leading to 4 candidates to further select out the more important candidate proteins for functional studies (see text). The four asterisks mark the proteins that are upregulated by heat-stress in thermotolerant strain <i>02</i>, not upregulated by heat stress in thermo-sensitive strain <i>8213,</i> and expressed at lower levels in thermo-sensitive strain <i>8213</i> than in the thermotolerant strain <i>02</i> with or without heat-stress. <i>02</i>-NS: <i>02</i> non-stressed (23°C/24 h); <i>02</i>-HS: <i>02</i> heat-stressed (33°C/24 h); <i>8213</i>-NS: <i>8213</i> non-stressed (23°C/24 h); <i>8213</i>-HS: strain <i>8213</i> heat-stressed (33°C/24 h).</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "classification", "differentially"], "article_id"=>956685, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g003", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Identification_and_classification_of_differentially_expressed_proteins_between_02_and_8213_/956685", "title"=>"Identification and classification of differentially expressed proteins between <i>02</i> and <i>8213</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413575"], "description"=>"<p>(A) MS/MS analysis identifies the protein spot 36 to be mushroom PABA synthase. (B) Differential transcription of PABA synthase gene <i>Pabs</i> in strain <i>02</i> and strain <i>8213</i> with or without heat stress. Strain <i>02</i> and strain <i>8213</i> were treated with high temperature at 33°C for the indicated time and the mRNA level of <i>Pabs</i> was quantified by real-time PCR. Three independent biological replicates were performed. Data are expressed as average ± SEM. Unpaired t-tests were performed between high temperature treatment samples and control samples (without treatment) within each strain, respectively, ns: P>0.05, *: P<0.05, **: P<0.01. (C) Differential protein accumulation of PABA synthase in strain <i>02</i> and strain <i>8213</i> after heat stress. Strain <i>02</i> and strain <i>8213</i> were treated with high temperature at 33°C for the indicated time, and PABA synthase protein levels were measured by western blot as indicated. (D) Differential accumulation of PABA content in strain <i>02</i> and strain <i>8213</i> after heat stress. Strain <i>02</i> and strain <i>8213</i> were treated with high temperature at 33°C for the indicated time and the content of PABA was measured by HPLC. Three independent biological replicates were performed. Data are expressed as average ± SEM.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "responded", "paba", "synthesis"], "article_id"=>956687, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g004", "stats"=>{"downloads"=>3, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Thermotolerant_strain_02_responded_to_heat_stress_with_more_PABA_synthesis_than_strain_8213_/956687", "title"=>"Thermotolerant strain <i>02</i> responded to heat stress with more PABA synthesis than strain <i>8213</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413577"], "description"=>"<p>(A) Strains <i>02</i> and <i>8213</i> mycelium culture were supplied with or without 5 mg/L PABA, followed by high temperature treatment at 33°C for 48 hours, the images were taken at the indicated time post-heat stress. The control groups were cultured under regular temperature of 23°C. (B) Dose-dependent protection by PABA of mycelium intactness under heat stress at 33°C for 48 hours. Strain <i>02</i> and strain <i>8213</i> were treated with different concentrations of PABA followed by high-temperature treatment at 33°C for 48 h, and the percentage of intact mycelia was counted. The intact mycelia percentage was calculated as compared to the control group (23°C). Three independent biological replicates were performed. Data are expressed as average ± SEM. Unpaired t-tests were performed between samples with and without PABA addition within each strain, respectively, ns: P>0.05, *: P<0.05, **: P<0.01.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "paba", "improves", "thermotolerance"], "article_id"=>956689, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g005", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Exogenous_PABA_improves_thermotolerance_of_strain_8213_/956689", "title"=>"Exogenous PABA improves thermotolerance of strain <i>8213</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413578"], "description"=>"<p>(A) Relative mRNA level of <i>Pabs</i> gene of strains <i>02</i>, <i>8213</i> and two <i>Pabs</i>-overexpressing transgenic strains <i>TB-2</i> and <i>TB-3</i> (derived from <i>8213</i>) under normal temperature (23°C) and heat stress (33°C). The mRNA of corresponding samples was extracted and analyzed after 24 hours of treatment. (B) The PABA content of strains <i>02</i>, <i>8213</i> and <i>TB-2</i> and <i>TB-3</i> under normal temperature (23°C) and heat stress (33°C) for 3 days. The PABA content of corresponding samples was extracted and measured after 3 days of treatment. (C) The mycelia growth of Strains <i>02</i>, <i>8213</i> and <i>TB-2</i> and <i>TB-3</i> under normal temperature (23°C) and heat stress (33°C). Mycelia cultures were photographed after 2 weeks of treatment. (D) The mycelia elongation of strains <i>02</i>, <i>8213</i> and <i>TB-2</i> and <i>TB-3</i> under normal temperature (23°C) and heat stress (33°C). The mycelia length is measured after 14 and 21 days of treatment. Three independent biological replicates were performed for each analysis. Data are expressed as average ± SEM. Unpaired t-tests were performed between strain <i>8213</i> and all other strains as indicated within each treatment condition, ns: P>0.05, *: P<0.05, **: P<0.01.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "overexpression", "paba", "synthase", "improves", "thermotolerance"], "article_id"=>956690, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g006", "stats"=>{"downloads"=>2, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transgenic_overexpression_of_PABA_synthase_improves_thermotolerance_of_strain_8213_/956690", "title"=>"Transgenic overexpression of PABA synthase improves thermotolerance of strain <i>8213</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413579"], "description"=>"<p>Mushrooms were treated with increasing concentrations of sulfanilamide for 6 days, and then subjected to heat stress (33°C) for 48 hours. PABA content (A) and the percentage of intact mycelium (B) were determined after heat stress treatment.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "synthase", "inhibitor", "sulfanilamide", "inhibits", "paba", "impairs", "mycelium"], "article_id"=>956691, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g007", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PABA_synthase_inhibitor_sulfanilamide_inhibits_PABA_production_and_impairs_mycelium_intactness_/956691", "title"=>"PABA synthase inhibitor sulfanilamide inhibits PABA production and impairs mycelium intactness.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413580"], "description"=>"<p>(A) Time course of accumulation of H<sub>2</sub>O<sub>2</sub> in the mycelia of strains <i>02</i>, <i>8213</i>, <i>TB-2</i> and <i>TB-3</i> under heat stress (33°C). Strains <i>02</i>, <i>TB-2</i> and <i>TB-3</i>, which produce more PABA content than strain <i>8213</i>, have less H<sub>2</sub>O<sub>2</sub> accumulation under parallel conditions. (B) Effects of PABA and PABA synthase inhibitor sulfanilamide on H<sub>2</sub>O<sub>2</sub> accumulation. Strain <i>02</i> and strain <i>8213</i> were cultured on PDA medium with 1 mM PABA or 0.1 mM sulfanilamide for 6 days, then were subjected to high temperature treatment (33°C) for 48 hours as indicated, followed by H<sub>2</sub>O<sub>2</sub> content measurement. Three independent biological replicates were performed for each analysis. Data are expressed as average ± SEM. Unpaired t-tests were performed between control sample and sulfanilamide treated sample or PABA treated sample within each strain, ns: P>0.05, *: P<0.05, **: P<0.01.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "accumulation", "stressed", "strains"], "article_id"=>956692, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g008", "stats"=>{"downloads"=>4, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PABA_reduces_H_2_O_2_accumulation_in_heat_stressed_strains_02_and_8213_/956692", "title"=>"PABA reduces H<sub>2</sub>O<sub>2</sub> accumulation in heat stressed strains <i>02</i> and <i>8213</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413581"], "description"=>"<p>Mushroom strains, as indicated, were treated with 1(33°C) for 2 days. The enzymes activities of Catalase and SOD were measured immediately after heat stress. Three independent biological replicates were performed for each analysis. Data are expressed as average ± SEM. Unpaired t-tests were performed as indicated in the figure, ns: P>0.05, *: P<0.05, **: P<0.01, Sul: sulfanilamide.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "sulfanilamide", "catalase", "sod"], "article_id"=>956693, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g009", "stats"=>{"downloads"=>2, "page_views"=>43, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PABA_increases_while_sulfanilamide_decreases_the_activity_of_Catalase_and_SOD_in_02_8213_and_TB_2_/956693", "title"=>"PABA increases, while sulfanilamide decreases, the activity of Catalase and SOD in <i>02</i>, <i>8213</i> and <i>TB-2</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413582"], "description"=>"<p>(A) Defense-related proteins HSPs and Chitinase accumulated more in thermotolerant strain <i>02</i> than in thermo-sensitive strain <i>8213</i> under heat stress (33°C). (B) Exogenous PABA (1 mM) application increases accumulation of HSPs and Chitinase in <i>8213</i> under heat stress (33°C). (C) PABA synthase inhibitor Sulfanilamide (0.1 mM) decreases accumulation of HSPs and Chitinase in <i>02</i> under heat stress (33°C). D) <i>Pabs</i>-overexpressing transgenic line <i>TB-2</i> accumulates more HSPs and Chitinase than the parent strain <i>8213</i> under heat stress (33°C). Three independent biological replicates were performed for each sample.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "mediates", "accumulation", "defense-related"], "article_id"=>956694, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g010", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PABA_mediates_the_accumulation_of_defense_related_proteins_in_02_8213_and_TB_2_under_heat_stress_/956694", "title"=>"PABA mediates the accumulation of defense-related proteins in <i>02</i>, <i>8213</i> and <i>TB-2</i> under heat stress.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413583"], "description"=>"<p>See Discussion for details.</p>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "paba", "enhancing", "thermotolerance"], "article_id"=>956695, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091298.g011", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_model_for_the_role_of_PABA_in_enhancing_thermotolerance_of_mushroom_/956695", "title"=>"Schematic model for the role of PABA in enhancing thermotolerance of mushroom.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:09:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413584", "https://ndownloader.figshare.com/files/1413585", "https://ndownloader.figshare.com/files/1413586", "https://ndownloader.figshare.com/files/1413587", "https://ndownloader.figshare.com/files/1413588"], "description"=>"<div><p>Most mushrooms are thermo-sensitive to temperatures over 23°C, which greatly restricts their agricultural cultivation. Understanding mushroom’s innate heat-tolerance mechanisms may facilitate genetic improvements of their thermotolerance. <i>Agaricus bisporus</i> strain <i>02</i> is a relatively thermotolerant mushroom strain, while strain <i>8213</i> is quite thermo-sensitive. Here, we compared their responses at proteomic level to heat treatment at 33°C. We identified 73 proteins that are differentially expressed between <i>02</i> and <i>8213</i> or induced upon heat stress in strain <i>02</i> itself, 48 of which with a known identity. Among them, 4 proteins are constitutively more highly expressed in <i>02</i> than <i>8213</i>; and they can be further upregulated in response to heat stress in <i>02,</i> but not in <i>8213.</i> One protein is encoded by the <i>para</i>-aminobenzoic acid (PABA) synthase gene <i>Pabs</i>, which has been shown to scavenge the reactive oxygen species <i>in vitro</i>. <i>Pabs</i> mRNA and its chemical product PABA show similar heat stress induction pattern as PABA synthase protein and are more abundant in <i>02</i>, indicating transcriptional level upregulation of <i>Pabs</i> upon heat stress. A specific inhibitor of PABA synthesis impaired thermotolerance of <i>02</i>, while exogenous PABA or transgenic overexpression of <i>02</i> derived PABA synthase enhanced thermotolerance of <i>8213</i>. Furthermore, compared to <i>8213</i>, <i>02</i> accumulated less H<sub>2</sub>O<sub>2</sub> but more defense-related proteins (e.g., HSPs and Chitinase) under heat stress. Together, these results demonstrate a role of PABA in enhancing mushroom thermotolerance by removing H<sub>2</sub>O<sub>2</sub> and elevating defense-related proteins.</p></div>", "links"=>[], "tags"=>["crops", "Biochemistry", "Nucleic acids", "rna", "proteins", "Plant science", "Plant biochemistry", "Plant cell biology", "Plant genetics", "Plant growth and development", "Plant physiology", "proteomics", "Protein abundance", "synthase", "thermotolerance"], "article_id"=>956696, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Zhonglei Lu", "Xiangxiang Kong", "Zhaoming Lu", "Meixiang Xiao", "Meiyuan Chen", "Liang Zhu", "Yuemao Shen", "Xiangyang Hu", "Siyang Song"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0091298.s001", "https://dx.doi.org/10.1371/journal.pone.0091298.s002", "https://dx.doi.org/10.1371/journal.pone.0091298.s003", "https://dx.doi.org/10.1371/journal.pone.0091298.s004", "https://dx.doi.org/10.1371/journal.pone.0091298.s005"], "stats"=>{"downloads"=>5, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Para_Aminobenzoic_Acid_PABA_Synthase_Enhances_Thermotolerance_of_Mushroom_Agaricus_bisporus_/956696", "title"=>"<i>Para</i>-Aminobenzoic Acid (PABA) Synthase Enhances Thermotolerance of Mushroom <i>Agaricus bisporus</i>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-03-10 03:09:04"}
PMC Usage Stats 144817 Feb 19:28 UTC

PMC Usage Stats | Further Information

  • {"unique-ip"=>"24", "full-text"=>"16", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"28", "full-text"=>"28", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"22", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"28", "full-text"=>"25", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"26", "full-text"=>"27", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"22", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"6", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"11", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"23", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"11", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"31", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"28", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"26", "full-text"=>"25", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"27", "full-text"=>"24", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"34", "full-text"=>"36", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"27", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"24", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"30", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"23", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"1", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"22", "full-text"=>"23", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"33", "full-text"=>"33", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"39", "full-text"=>"38", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"38", "full-text"=>"42", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"23", "full-text"=>"20", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"34", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"13", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"12"}
Relative Metric 167710 May 17:33 UTC

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Engineering and technology", "average_usage"=>[282]}, {"subject_area"=>"/Medicine and health sciences/Nutrition", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[287]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[266]}]}
Loading … Spinner
There are currently no alerts