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{"title"=>"Sphingosine 1-phosphate (S1P) induced interleukin-8 (IL-8) release is mediated by S1P receptor 2 and nuclear factor kB in BEAS-2B cells", "type"=>"journal", "authors"=>[{"first_name"=>"Michael J.", "last_name"=>"O'Sullivan", "scopus_author_id"=>"55792149200"}, {"first_name"=>"Nobuaki", "last_name"=>"Hirota", "scopus_author_id"=>"14045011200"}, {"first_name"=>"James G.", "last_name"=>"Martin", "scopus_author_id"=>"35493942900"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24743449", "doi"=>"10.1371/journal.pone.0095566", "pui"=>"372999731", "issn"=>"19326203", "sgr"=>"84899738610", "scopus"=>"2-s2.0-84899738610"}, "id"=>"4e3b167e-5eea-3985-b49c-74a6afb20ccd", "abstract"=>"The airway epithelium may release pro-inflammatory cytokines and chemokines in the asthmatic airway. Sphingosine 1-phosphate (S1P) is a bioactive lipid, increased in the airways of asthmatics, that may trigger the release of the potent neutrophil chemoattractant Interleukin-8 (IL-8) by epithelial cells. S1P is a ligand for 5 G protein-coupled receptors, S1PR1-5. We wished to explore the mechanisms of S1P induced IL-8 secretion with regard to the receptor(s) and downstream signaling events involved. Our results indicate that S1P induced IL-8 release is mediated by S1PR2 and the transcription factor NF-kB. Since the Epidermal Growth Factor Receptor (EGFR) and reactive oxygen species (ROS) have been implicated in IL-8 release in response to activation of other G protein-coupled receptors, we examined their importance in S1P induced IL-8 release and established that they are not involved. This study reveals S1PR2 and NF-kB as potential therapeutic targets in neutrophilic airway diseases such as severe asthma.", "link"=>"http://www.mendeley.com/research/sphingosine-1phosphate-s1p-induced-interleukin8-il8-release-mediated-s1p-receptor-2-nuclear-factor-k", "reader_count"=>6, "reader_count_by_academic_status"=>{"Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>2, "Other"=>1}, "reader_count_by_user_role"=>{"Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>2, "Other"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Iran"=>1}, "group_count"=>0}

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• http://doi.org/10.3390/ijms19010112
• http://doi.org/10.1128/MCB.00554-15
• http://doi.org/10.1002/jcla.22927
• http://doi.org/10.1186/s12866-017-1081-8
• http://doi.org/10.1371/journal.pone.0119005
• http://doi.org/10.1038/s41598-017-02104-5
• http://doi.org/10.1016/j.transproceed.2019.05.012
• http://doi.org/10.1016/j.prostaglandins.2019.106365
• http://doi.org/10.1016/j.pharmthera.2018.07.006
• http://doi.org/10.1016/j.placenta.2015.08.010
• http://doi.org/10.1371/journal.pone.0203211
• http://doi.org/10.3389/fphar.2016.00167

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Figshare

• {"files"=>["https://ndownloader.figshare.com/files/1468089"], "description"=>"<p>(A) BEAS-2B cells were incubated for 30 minutes with 10 µM DCFH-DA. Free probe was washed with Hank’s buffer and baseline fluorescence was measured at 530 nm. Cells were then stimulated with S1P or vehicle and fluorescence intensity was measured every 5 minutes for one hour (n = 3). Analysis using repeated measures ANOVA revealed no difference between the two curves. (B) BEAS-2B cells were pretreated for 30 minutes with the general antioxidant N-acetyl cysteine (NAC) and then stimulated with S1P for 4 hours (n = 5). Culture medium was assessed for IL-8 concentration by ELISA. (C) BEAS-2B cells were pretreated for 30 minutes with the NADPH oxidase inhibitor DPI and then stimulated with S1P for 4 hours (n = 5). Culture medium was assessed for IL-8 concentration by ELISA. (n = 5). Data are representative of means+SE. ANOVA with Tukey post hoc pairwise comparisons *P<0.05, **P<0.01.</p>", "links"=>[], "tags"=>["anatomy", "biological tissue", "epithelium", "Epithelial cells", "Endocrine system", "Biochemistry", "lipids", "Lipid mediators", "cell biology", "Cell processes", "Cell cycle and cell division", "cytokinesis", "Signal transduction", "cell signaling", "G-protein signaling", "Molecular cell biology", "developmental biology", "Molecular development", "cytokines", "immunology", "Immune system", "physiology", "Endocrine physiology", "Growth factors", "Epidermal growth factor", "Immune physiology", "Respiratory physiology", "Pulmonology", "asthma", "induced", "il-8", "reactive", "beas-2b"], "article_id"=>1002285, "categories"=>["Biological Sciences"], "users"=>["Michael J. O’Sullivan", "Nobuaki Hirota", "James G. Martin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095566.g006", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_S1P_induced_IL_8_release_is_not_dependent_on_the_production_of_reactive_oxygen_species_in_BEAS_2B_cells_/1002285", "title"=>"S1P induced IL-8 release is not dependent on the production of reactive oxygen species in BEAS-2B cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-17 02:51:45"}
• {"files"=>["https://ndownloader.figshare.com/files/1468088"], "description"=>"<p>(A) BEAS-2B cells were pretreated for 30 minutes with the specific EGFR inhibitor AG 1478 and then stimulated with S1P for 4 hours (n = 7). Culture medium was assessed for IL-8 concentration by ELISA. (B) BEAS-2B cells transfected with control or EGFR specific siRNA were stimulated with S1P for 4 hours (n = 3). Culture medium was assessed for concentrations of IL-8 by ELISA (left). Knock-down efficacy is shown by western blot for total EGFR (170 kDa) with GAPDH loading control (37 kDa) (right). Quantification of total EGFR bands is shown (n = 4) (bottom left). (C) BEAS-2B cells were pretreated for 30 minutes with MMP inhibitors GM6001 or TAPI-1 and then stimulated with S1P for 4 hours (n = 3). Culture medium was assessed for IL-8 concentration by ELISA. Data are representative of means+SE. ANOVA with Tukey post hoc pairwise comparisons. *P<0.05, **P<0.01, ***P<0.001.</p>", "links"=>[], "tags"=>["anatomy", "biological tissue", "epithelium", "Epithelial cells", "Endocrine system", "Biochemistry", "lipids", "Lipid mediators", "cell biology", "Cell processes", "Cell cycle and cell division", "cytokinesis", "Signal transduction", "cell signaling", "G-protein signaling", "Molecular cell biology", "developmental biology", "Molecular development", "cytokines", "immunology", "Immune system", "physiology", "Endocrine physiology", "Growth factors", "Epidermal growth factor", "Immune physiology", "Respiratory physiology", "Pulmonology", "asthma", "induced", "il-8", "egfr", "beas-2b"], "article_id"=>1002284, "categories"=>["Biological Sciences"], "users"=>["Michael J. O’Sullivan", "Nobuaki Hirota", "James G. Martin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095566.g005", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_S1P_induced_IL_8_release_is_not_dependent_on_the_EGFR_in_BEAS_2B_cells_/1002284", "title"=>"S1P induced IL-8 release is not dependent on the EGFR in BEAS-2B cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-17 02:51:45"}
• {"files"=>["https://ndownloader.figshare.com/files/1468085"], "description"=>"<p>BEAS-2B cells were pretreated for 30 minutes with (A) W 123 (n = 5), (B) JTE 013 (n = 5) or (C) CAY 10444 (n = 8), specific inhibitors of S1PR1, S1PR2 and S1PR3 respectively and then stimulated with S1P or vehicle for 4 hours. Culture supernatant was analyzed for IL-8 concentration by ELISA. Human airway smooth muscle cells were loaded with 10 µM Fura 2-AM, then treated with CAY10444 or vehicle (n = 5) for 30 minutes and intracellular calcium was measured by ratiometric fluorescence microscopy (E). Cells were stimulated with S1P (n = 5) and increases in resting intracellular calcium were recorded (F). Data are representative of means+SE. ANOVA with Tukey post hoc pairwise comparisons. **P<0.01, ***P<0.001.</p>", "links"=>[], "tags"=>["anatomy", "biological tissue", "epithelium", "Epithelial cells", "Endocrine system", "Biochemistry", "lipids", "Lipid mediators", "cell biology", "Cell processes", "Cell cycle and cell division", "cytokinesis", "Signal transduction", "cell signaling", "G-protein signaling", "Molecular cell biology", "developmental biology", "Molecular development", "cytokines", "immunology", "Immune system", "physiology", "Endocrine physiology", "Growth factors", "Epidermal growth factor", "Immune physiology", "Respiratory physiology", "Pulmonology", "asthma", "induced", "il-8", "mediated", "s1pr2", "beas-2b"], "article_id"=>1002281, "categories"=>["Biological Sciences"], "users"=>["Michael J. O’Sullivan", "Nobuaki Hirota", "James G. Martin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095566.g002", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_S1P_induced_IL_8_release_is_mediated_by_S1PR2_in_BEAS_2B_cells_/1002281", "title"=>"S1P induced IL-8 release is mediated by S1PR2 in BEAS-2B cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-17 02:51:45"}
• {"files"=>["https://ndownloader.figshare.com/files/1468084"], "description"=>"<p>BEAS-2B cells were stimulated with various concentrations of S1P for 4 hours. Culture supernatant was analyzed for concentration of IL-8 by ELISA (n = 5). Data are representative of means+SE. ANOVA with Tukey post hoc pairwise comparisons. *P<0.05.</p>", "links"=>[], "tags"=>["anatomy", "biological tissue", "epithelium", "Epithelial cells", "Endocrine system", "Biochemistry", "lipids", "Lipid mediators", "cell biology", "Cell processes", "Cell cycle and cell division", "cytokinesis", "Signal transduction", "cell signaling", "G-protein signaling", "Molecular cell biology", "developmental biology", "Molecular development", "cytokines", "immunology", "Immune system", "physiology", "Endocrine physiology", "Growth factors", "Epidermal growth factor", "Immune physiology", "Respiratory physiology", "Pulmonology", "asthma", "il-8", "beas-2b"], "article_id"=>1002280, "categories"=>["Biological Sciences"], "users"=>["Michael J. O’Sullivan", "Nobuaki Hirota", "James G. Martin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095566.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_S1P_induces_IL_8_release_in_BEAS_2B_cells_/1002280", "title"=>"S1P induces IL-8 release in BEAS-2B cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-17 02:51:45"}
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• {"files"=>["https://ndownloader.figshare.com/files/1468086"], "description"=>"<p>BEAS-2B cells were pretreated with (A) Helenalin (n = 3), (B) SR 11302 (n = 3), or c-JUN peptide (n = 3) inhibitors of NF-κB, AP-1 and AP-1 respectively, for 30 minutes and then stimulated with S1P for 4 hours. Culture supernatants were analyzed for IL-8 concentration by ELISA. Data are representative of means+SE. ANOVA with Tukey post hoc pairwise comparisons. *P<0.05, ***P<0.001.</p>", "links"=>[], "tags"=>["anatomy", "biological tissue", "epithelium", "Epithelial cells", "Endocrine system", "Biochemistry", "lipids", "Lipid mediators", "cell biology", "Cell processes", "Cell cycle and cell division", "cytokinesis", "Signal transduction", "cell signaling", "G-protein signaling", "Molecular cell biology", "developmental biology", "Molecular development", "cytokines", "immunology", "Immune system", "physiology", "Endocrine physiology", "Growth factors", "Epidermal growth factor", "Immune physiology", "Respiratory physiology", "Pulmonology", "asthma", "induced", "il-8", "mediated", "beas-2b"], "article_id"=>1002282, "categories"=>["Biological Sciences"], "users"=>["Michael J. O’Sullivan", "Nobuaki Hirota", "James G. Martin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095566.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_S1P_induced_IL_8_release_is_mediated_by_NF_954_B_in_BEAS_2B_cells_/1002282", "title"=>"S1P induced IL-8 release is mediated by NF-κB in BEAS-2B cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-17 02:51:45"}

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• {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
• {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
• {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
• {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Immunology", "average_usage"=>[289]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[271]}, {"subject_area"=>"/Physical sciences/Mathematics", "average_usage"=>[286]}]}

F1000Prime