Novel Approach to Activity Evaluation for Release-Active Forms of Anti-Interferon-Gamma Antibodies Based on Enzyme-Linked Immunoassay
- Publication Date
- May 09, 2014
- Journal
- PLOS ONE
- Authors
- Elena S. Gavrilova, Sergey A. Bobrovnik, Gordon Sherriff, Andrey A. Myslivets, et al
- Volume
- 9
- Issue
- 5
- Pages
- e97017
- DOI
- https://dx.plos.org/10.1371/journal.pone.0097017
- Publisher URL
- http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0097017
- PubMed
- http://www.ncbi.nlm.nih.gov/pubmed/24816648
- PubMed Central
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016219
- Europe PMC
- http://europepmc.org/abstract/MED/24816648
- Web of Science
- 000336838000095
- Scopus
- 84901207706
- Mendeley
- http://www.mendeley.com/research/novel-approach-activity-evaluation-releaseactive-forms-antiinterferongamma-antibodies-based-enzymeli
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Mendeley | Further Information
{"title"=>"Novel approach to activity evaluation for release-active forms of anti-interferon-gamma antibodies based on enzyme-linked immunoassay", "type"=>"journal", "authors"=>[{"first_name"=>"Elena S.", "last_name"=>"Gavrilova", "scopus_author_id"=>"56176033300"}, {"first_name"=>"Sergey A.", "last_name"=>"Bobrovnik", "scopus_author_id"=>"7005428351"}, {"first_name"=>"Gordon", "last_name"=>"Sherriff", "scopus_author_id"=>"56175986300"}, {"first_name"=>"Andrey A.", "last_name"=>"Myslivets", "scopus_author_id"=>"56175433200"}, {"first_name"=>"Sergey A.", "last_name"=>"Tarasov", "scopus_author_id"=>"7005125924"}, {"first_name"=>"Oleg I.", "last_name"=>"Epstein", "scopus_author_id"=>"7005380941"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84901207706", "pmid"=>"24816648", "doi"=>"10.1371/journal.pone.0097017", "pui"=>"373132260", "issn"=>"19326203", "scopus"=>"2-s2.0-84901207706"}, "id"=>"8d3349fd-3d20-348f-ac30-b941ff044a29", "abstract"=>"Selection of a suitable assay to measure the activity of drug agents based on release-active forms of anti-interferon-gamma antibodies (RA forms of Abs) is an important step forward in the investigation of such agents. In this study, the enzyme-linked immunosorbent assay was utilized to examine the effect of RA forms of Abs specific for human interferon gamma on the interaction between monoclonal anti-interferon gamma antibodies and recombinant human interferon gamma. The experimental data and the results obtained by using relevant mathematical analysis showed that such RA forms of Abs are able to modulate the monoclonal antibody interaction with both soluble and immobilized (to the assay plate well) interferon gamma. These data demonstrated the importance of using relatively low concentrations of both soluble and plate-immobilized interferon gamma to detect the effects of RA forms of Abs to interferon gamma on the binding of monoclonal antibodies to interferon gamma. It has been suggested that the observed influence of RA forms of Abs on 'antibody-antigen' interaction could be used to detect and analyze the activity of drugs containing RA forms of Abs.", "link"=>"http://www.mendeley.com/research/novel-approach-activity-evaluation-releaseactive-forms-antiinterferongamma-antibodies-based-enzymeli", "reader_count"=>9, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>4}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "group_count"=>2}CrossRef
- http://doi.org/10.15407/ubj91.01.030
- http://doi.org/10.3390/s16010096
- http://doi.org/10.3390/sym10030053
- http://doi.org/10.1136/bmjebm-2018-111121
- http://doi.org/10.1371/journal.pone.0197086
- http://doi.org/10.1016/j.ijid.2016.09.002
- http://doi.org/10.3390/sym10040103
- http://doi.org/10.1002/jmv.24717
- http://doi.org/10.1016/j.ijid.2019.09.030
- http://doi.org/10.1002/jmv.24759
Scopus | Further Information
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Relative Metric
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