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{"title"=>"Modulation of membrane lipid composition and homeostasis in salmon hepatocytes exposed to hypoxia and perfluorooctane sulfonamide, given singly or in combination", "type"=>"journal", "authors"=>[{"first_name"=>"Marianne", "last_name"=>"Olufsen", "scopus_author_id"=>"54584131000"}, {"first_name"=>"Maria V.", "last_name"=>"Cangialosi", "scopus_author_id"=>"7801532681"}, {"first_name"=>"Augustine", "last_name"=>"Arukwe", "scopus_author_id"=>"7003567723"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"373577969", "pmid"=>"25047721", "doi"=>"10.1371/journal.pone.0102485", "scopus"=>"2-s2.0-84904572549", "issn"=>"19326203", "sgr"=>"84904572549"}, "id"=>"b2268ad4-f403-3f6e-bc3e-a9e1ea7b6194", "abstract"=>"The relative importance of environmental hypoxia due to global climate change on organismal ability to adapt to chemical insult and/or mechanisms of these responses is not well understood. Therefore, we have studied the effects of combined exposure to perfluorooctane sulfonamide (PFOSA) and chemically induced hypoxia on membrane lipid profile and homeostasis. Primary salmon hepatocytes were exposed to PFOSA at 0, 25 and 50 µM singly or in combination with either cobalt chloride (CoCl2: 0 and 150 µM) or deferroxamine (DFO: 0 and 100 µM) for 24 and 48 h. CoCl2 and DFO were used to induce cellular hypoxia because these two chemicals have been commonly used in animal experiments for this purpose and have been shown to increase hypoxia-inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF) levels. Fatty acid (FA) profiles were determined by GC-MS, while gene expression patterns were determined by quantitative PCR. Hypoxic condition was confirmed with time-related increases of HIF-1α mRNA levels in CoCl2 and DFO exposed cells. In general, significant alterations of genes involved in lipid homeostasis were predominantly observed after 48 h exposure. Gene expression analysis showed that biological responses related to peroxisome proliferation (peroxisome proliferator-activated receptors (PPARs) and acyl coenzyme A (ACOX)) and FA desaturation (Δ5- and Δ6-desaturases: FAD5 and FAD6, respectively) and elongation (FAE) were elevated slightly by single exposure (i.e. either PFOSA, CoCl2 or DFO exposure alone), and these responses were potentiated in combined exposure conditions. Principal component analysis (PCA) showed a clustering of peroxisome proliferation responses at transcript levels and FA desaturation against membrane FAs levels whose changes were explained by PFOSA and chemically induced hypoxia exposures. Overall, our data show that most of the observed responses were stronger in combined stressor exposure conditions, compared to individual stressor exposure. In general, our data show that hypoxia may, singly or in combination with PFOSA produce deleterious health, physiological and developmental consequences through the alteration of membrane lipid profile in organisms.", "link"=>"http://www.mendeley.com/research/modulation-membrane-lipid-composition-homeostasis-salmon-hepatocytes-exposed-hypoxia-perfluorooctane", "reader_count"=>11, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Student > Bachelor"=>1, "Professor"=>4}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>1, "Student > Master"=>3, "Student > Bachelor"=>1, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Environmental Science"=>2, "Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>4, "Agricultural and Biological Sciences"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Environmental Science"=>{"Environmental Science"=>2}}, "group_count"=>1}

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• http://doi.org/10.1016/j.envres.2017.07.024
• http://doi.org/10.1016/j.cbi.2015.08.006
• http://doi.org/10.1016/j.intimp.2016.06.034
• http://doi.org/10.1016/j.marenvres.2017.06.003
• http://doi.org/10.1016/j.tox.2016.11.003
• http://doi.org/10.1080/1354750X.2017.1315614
• http://doi.org/10.1007/s00227-016-3037-1
• http://doi.org/10.1016/j.envpol.2016.07.005
• http://doi.org/10.1016/j.scitotenv.2017.11.118
• http://doi.org/10.1007/s11356-014-3847-y
• http://doi.org/10.1016/j.cbpc.2016.08.009

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• {"files"=>["https://ndownloader.figshare.com/files/1604123"], "description"=>"<p>*Sequences are given in the 5′−3′order</p>", "links"=>[], "tags"=>["organisms", "animals", "vertebrates", "fishes", "physiology", "toxicology", "Toxic agents", "Atmospheric science", "Climatology", "climate change", "accession", "amplicon", "annealing", "genes", "Real-time"], "article_id"=>1113589, "categories"=>["Biological Sciences"], "users"=>["Marianne Olufsen", "Maria V. Cangialosi", "Augustine Arukwe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102485.t001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Primer_pair_sequences_accession_numbers_amplicon_size_and_annealing_temperature_conditions_for_genes_of_interest_used_for_real_time_PCR_/1113589", "title"=>"Primer pair sequences, accession numbers, amplicon size and annealing temperature conditions for genes of interest used for real-time PCR.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-07-21 15:33:09"}
• {"files"=>["https://ndownloader.figshare.com/files/1604119"], "description"=>"<p>Transcripts were analyzed using real-time polymerase chain reaction (qPCR) and expressed as mean percentage (%) of control ± SEM (n = 5). Asterisk (*) denotes significant difference (p<0.05) compared to control analyzed by Tukey’s test.</p>", "links"=>[], "tags"=>["organisms", "animals", "vertebrates", "fishes", "physiology", "toxicology", "Toxic agents", "Atmospheric science", "Climatology", "climate change", "acox", "mrna", "hepatocytes", "exposed", "dfo", "pfosa", "50"], "article_id"=>1113585, "categories"=>["Biological Sciences"], "users"=>["Marianne Olufsen", "Maria V. Cangialosi", "Augustine Arukwe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102485.g003", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcriptional_changes_of_ACOX_mRNA_in_salmon_hepatocytes_exposed_to_CoCl_2_150_181_M_or_DFO_100_181_M_in_presence_and_absence_of_PFOSA_25_and_50_181_M_/1113585", "title"=>"Transcriptional changes of ACOX mRNA in salmon hepatocytes exposed to CoCl₂ (150 µM) or DFO (100 µM), in presence and absence of PFOSA (25 and 50 µM).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-21 15:33:09"}
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• {"files"=>["https://ndownloader.figshare.com/files/1604118"], "description"=>"<p>Transcripts were analyzed using real-time polymerase chain reaction (qPCR) and expressed as mean percentage (%) of control ± SEM (n = 5). Asterisk (*) denotes significant difference (p<0.05) compared to control analyzed by Tukey’s test, while diamond (^♦) denotes significant difference (p<0.05) with individual hypoxia treatment group (CoCl₂ or DFO) at respective time-interval.</p>", "links"=>[], "tags"=>["organisms", "animals", "vertebrates", "fishes", "physiology", "toxicology", "Toxic agents", "Atmospheric science", "Climatology", "climate change", "fad5", "fad6", "fae", "hepatocytes", "exposed", "dfo", "singly", "pfosa", "50"], "article_id"=>1113584, "categories"=>["Biological Sciences"], "users"=>["Marianne Olufsen", "Maria V. Cangialosi", "Augustine Arukwe"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0102485.g002", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Modulation_of_FAD5_A_FAD6_B_and_FAE_C_in_salmon_hepatocytes_exposed_to_CoCl_2_150_181_M_or_DFO_100_181_M_singly_or_in_combination_with_PFOSA_25_and_50_181_M_/1113584", "title"=>"Modulation of FAD5 (A), FAD6 (B) and FAE (C) in salmon hepatocytes exposed to CoCl₂ (150 µM) or DFO (100 µM), singly or in combination with PFOSA (25 and 50 µM).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-07-21 15:33:09"}
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PMC Usage Stats | Further Information

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Relative Metric

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F1000Prime