MafA Is Required for Postnatal Proliferation of Pancreatic β-Cells
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"MafA is required for postnatal proliferation of pancreatic β-cells", "type"=>"journal", "authors"=>[{"first_name"=>"Koki", "last_name"=>"Eto", "scopus_author_id"=>"55580823100"}, {"first_name"=>"Wataru", "last_name"=>"Nishimura", "scopus_author_id"=>"6602193756"}, {"first_name"=>"Hisashi", "last_name"=>"Oishi", "scopus_author_id"=>"7006227169"}, {"first_name"=>"Haruhide", "last_name"=>"Udagawa", "scopus_author_id"=>"54401760000"}, {"first_name"=>"Miho", "last_name"=>"Kawaguchi", "scopus_author_id"=>"8935993200"}, {"first_name"=>"Masaki", "last_name"=>"Hiramoto", "scopus_author_id"=>"7005189075"}, {"first_name"=>"Toshiyoshi", "last_name"=>"Fujiwara", "scopus_author_id"=>"35407437200"}, {"first_name"=>"Satoru", "last_name"=>"Takahashi", "scopus_author_id"=>"56132080000"}, {"first_name"=>"Kazuki", "last_name"=>"Yasuda", "scopus_author_id"=>"7403377987"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84911455995", "pmid"=>"25390658", "sgr"=>"84911455995", "doi"=>"10.1371/journal.pone.0104184", "issn"=>"19326203", "pui"=>"373772252"}, "id"=>"b462421a-ec39-36c2-a32b-bd5c55892493", "abstract"=>"[This corrects the article DOI: 10.1371/journal.pone.0104184.].", "link"=>"http://www.mendeley.com/research/mafa-required-postnatal-proliferation-pancreatic-%CE%B2cells", "reader_count"=>21, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>6, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>3, "Student > Ph. D. Student"=>6, "Student > Master"=>3, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>7, "Medicine and Dentistry"=>3, "Agricultural and Biological Sciences"=>8}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>8}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84911455995"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84911455995?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84911455995&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84911455995&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84911455995", "dc:identifier"=>"SCOPUS_ID:84911455995", "eid"=>"2-s2.0-84911455995", "dc:title"=>"MafA is required for postnatal proliferation of pancreatic β-cells", "dc:creator"=>"Eto K.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-08-15", "prism:coverDisplayDate"=>"15 August 2014", "prism:doi"=>"10.1371/journal.pone.0104184", "citedby-count"=>"15", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Okayama University", "affiliation-city"=>"Okayama", "affiliation-country"=>"Japan"}, {"@_fa"=>"true", "affilname"=>"Research Institute", "affiliation-city"=>"Tokyo", "affiliation-country"=>"Japan"}], "pubmed-id"=>"25126749", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e104184", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0104184", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"75", "xml_views"=>"17", "html_views"=>"370"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"38", "xml_views"=>"1", "html_views"=>"102"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"3", "html_views"=>"69"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"5", "html_views"=>"72"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"28", "xml_views"=>"2", "html_views"=>"56"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1637662"], "description"=>"<p>Genes that were downregulated in MafA KO islets in both transcriptome datasets.</p>", "links"=>[], "tags"=>["target Cyclin D 2", "5b", "postnatal proliferation", "expression", "MafA knockout islets", "prlr", "insulin gene transcription factor"], "article_id"=>1140574, "categories"=>["Biological Sciences"], "users"=>["Koki Eto", "Wataru Nishimura", "Hisashi Oishi", "Haruhide Udagawa", "Miho Kawaguchi", "Masaki Hiramoto", "Toshiyoshi Fujiwara", "Satoru Takahashi", "Kazuki Yasuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104184.t001", "stats"=>{"downloads"=>8, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genes_that_were_downregulated_in_MafA_KO_islets_in_both_transcriptome_datasets_/1140574", "title"=>"Genes that were downregulated in MafA KO islets in both transcriptome datasets.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-08-15 02:54:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1637659"], "description"=>"<p>The effects of MafA expression on <i>Prlr</i> promoter activity were analyzed using HeLa cells. (A) Luciferase activity driven by the <i>hPrlr</i> promoter (from −890 to +103) in MafA-overexpressing cells compared to control cells transfected with the control vector pcDNA3.1. n = 8. (B) Luciferase activities driven by the <i>mPrlr</i> promoter constructs, including −2232 to +127 (<i>mPrlrP</i>-1), −1177 to +127 (<i>mPrlrP</i>-2) and −481 to +127 (<i>mPrlrP</i>-3), with or without overexpression of MafA, relative to the luciferase activity of <i>mPrlrP</i>-1 with control. n = 5. Six putative MAREs were observed in the <i>mPrlr</i> promoter and are referred to as region I (from −2026 to −2016), region II (from −1907 to −1897), region III (from −1723 to −1713), region IV (from −217 to −207), region V (from −47 to −37) and region VI (from −12 to −2). TSS: transcription start site. (C) Luciferase activities of the <i>mPrlrP-1</i> construct (−2232 to +127) with MafA compared to the luciferase activities of constructs with deletions in the following regions: region VI (from −12 to −2, <i>mPlrlP</i>-11), region V (from −47 to −37, <i>mPlrlP</i>-5), region IV (from −217 to −207, <i>mPlrlP</i>-6), from −800 to −535 (<i>mPlrlP</i>-9) or regions I to III (from −2026 to −1409, <i>mPlrlP</i>-8). n = 4. The data represent the mean ± S.E.M. Asterisks means p<0.05.</p>", "links"=>[], "tags"=>["target Cyclin D 2", "5b", "postnatal proliferation", "expression", "MafA knockout islets", "prlr", "insulin gene transcription factor"], "article_id"=>1140571, "categories"=>["Biological Sciences"], "users"=>["Koki Eto", "Wataru Nishimura", "Hisashi Oishi", "Haruhide Udagawa", "Miho Kawaguchi", "Masaki Hiramoto", "Toshiyoshi Fujiwara", "Satoru Takahashi", "Kazuki Yasuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104184.g002", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Activation_of_the_Prlr_promoter_by_MafA_/1140571", "title"=>"Activation of the <i>Prlr</i> promoter by MafA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-15 02:54:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1637658"], "description"=>"<p>(A) mRNA expression of the indicated molecules in MIN6 cells transfected with <i>MafA</i> siRNA relative to cells transfected with control siRNA. n = 4 for each molecule, except n = 3 for <i>MafB</i>. Black bars: molecules detected in the transcriptome of <i>MafA</i> KO islets; white bars: molecules involved in β-cell function. (B) qRT-PCR analysis of prolactin signaling components in <i>MafA</i> KO islets relative to wild-type islets. n = 5 for Prlr; n = 3 for <i>Jak2</i>, <i>Stat5A</i> and <i>Stat5B</i>. (C) qRT-PCR analysis of molecules involved in the cell cycle in <i>MafA</i> KO islets relative to wild-type islets. n = 4 for <i>Ccnd1</i>; n = 5 for <i>Ccnd2</i> and <i>Ccnd3</i>; n = 3 for <i>Cdkn1a</i>, <i>Cdkn1b</i> and <i>Cdkn1c</i>. (D) Immunoblots of Prlr and actin in wild-type (WT) and <i>MafA</i> KO (KO) islets. n = 3. (E) Immunoblots of Ccnd2 and actin in wild-type (WT) and <i>MafA</i> KO (KO) islets. n = 3. (F) Quantification of the results of (D) and (E). The data represent the mean ± S.E.M. in (A), (B), (C) and (F), and representative results are shown in (D) and (E). Asterisks means p<0.05.</p>", "links"=>[], "tags"=>["target Cyclin D 2", "5b", "postnatal proliferation", "expression", "MafA knockout islets", "prlr", "insulin gene transcription factor"], "article_id"=>1140570, "categories"=>["Biological Sciences"], "users"=>["Koki Eto", "Wataru Nishimura", "Hisashi Oishi", "Haruhide Udagawa", "Miho Kawaguchi", "Masaki Hiramoto", "Toshiyoshi Fujiwara", "Satoru Takahashi", "Kazuki Yasuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104184.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibition_of_MafA_expression_in_cell_lines_or_mouse_islets_resulted_in_reduced_expression_of_Prlr_and_Ccnd2_/1140570", "title"=>"Inhibition of <i>MafA</i> expression in β-cell lines or mouse islets resulted in reduced expression of Prlr and Ccnd2.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-15 02:54:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1637661"], "description"=>"<p>(A, B) Representative islets of <i>MafA</i> KO mice and their wild-type littermates 24 hours after the injection of BrdU (100 mg BrdU/kg body weight). BrdU (green), insulin (red) and DAPI (blue). (C) Quantification of the BrdU<sup>+</sup> cells among the insulin<sup>+</sup> cells. In total, 1706 insulin<sup>+</sup> cells were counted in the <i>MafA</i> KO pancreas, and 1262 insulin<sup>+</sup> cells were counted in the wild-type pancreas. The proliferation of β-cells in <i>MafA</i> KO mice was impaired. n = 3. The data represent the mean ± S.E.M. Bar, 20 µm.</p>", "links"=>[], "tags"=>["target Cyclin D 2", "5b", "postnatal proliferation", "expression", "MafA knockout islets", "prlr", "insulin gene transcription factor"], "article_id"=>1140573, "categories"=>["Biological Sciences"], "users"=>["Koki Eto", "Wataru Nishimura", "Hisashi Oishi", "Haruhide Udagawa", "Miho Kawaguchi", "Masaki Hiramoto", "Toshiyoshi Fujiwara", "Satoru Takahashi", "Kazuki Yasuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104184.g004", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Impaired_proliferation_of_cells_in_MafA_KO_mice_/1140573", "title"=>"Impaired proliferation of β-cells in <i>MafA</i> KO mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-15 02:54:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1637660"], "description"=>"<p>(A, B) mRNA expression of the indicated molecules in INS-1 cells transfected with siRNA targeting <i>MafA</i> (A) or <i>Prlr</i> (B) relative to INS-1 cells transfected with control siRNA. n = 4 for both (A) and (B). (C–P) INS-1 cells were transfected with control siRNA or siRNA targeting <i>Prlr</i> (si-<i>Prlr</i>) or <i>MafA</i> (si-<i>MafA</i>). At 48 hours after transfection, the cells were cultured in defined serum-free medium for 24 hours for serum deprivation, followed by the addition of prolactin (PRL) or PBS as a control. (C, D) The immunoprecipitates obtained with anti-Stat5B antibody from 1% TX-100 soluble fractions of INS-1 cells transfected with control si-RNA, si-<i>Prlr</i> or si-<i>MafA</i>, and with or without PRL stimulation were immunoblotted with either anti-Stat5B (C) or anti-phosphotyrosine antibody (D). n = 3. (E, F) 1% TX-100 soluble fractions of INS-1 cell lysates were immunoblotted with either anti-Stat5B (E) or anti-phosphorylated Stat5 antibody (F). n = 3. (G) Dose-dependent effects of PRL on the phosphorylation of Stat5. n = 2. (H–K) Localization of Stat5B (red), β-catenin (green) and nuclei, which were counterstained with DAPI (blue), in INS-1 cells that were transfected or not with si-<i>Prlr</i> and stimulated or not with PRL. n = 3. (L–N) Localization of Stat5B (red), β-catenin (green) and nuclei, which were counterstained with DAPI (blue), in INS-1 cells with or without PRL stimulation and with or without exposure to AG490, a Jak2 inhibitor. n = 3. (O, P) The amount of phosphorylated Stat5, Ccnd2 and Lamin A/C (control) in the nuclear fraction of INS-1 cells with (w/) or without (w/o) PRL stimulation after serum deprivation (O), and quantification of the results (P). n = 4. (Q) mRNA expression of the indicated molecules in INS-1 cells transfected with siRNA targeting <i>Prlr</i> relative to INS-1 cells transfected with control siRNA. n = 3 for each molecule. The data represent the mean ± S.E.M. in (A), (B), (P) and (Q), and representative results are shown in (C–O). Asterisks means p<0.05.</p>", "links"=>[], "tags"=>["target Cyclin D 2", "5b", "postnatal proliferation", "expression", "MafA knockout islets", "prlr", "insulin gene transcription factor"], "article_id"=>1140572, "categories"=>["Biological Sciences"], "users"=>["Koki Eto", "Wataru Nishimura", "Hisashi Oishi", "Haruhide Udagawa", "Miho Kawaguchi", "Masaki Hiramoto", "Toshiyoshi Fujiwara", "Satoru Takahashi", "Kazuki Yasuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104184.g003", "stats"=>{"downloads"=>3, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Regulation_of_Ccnd2_expression_by_prolactin_signaling_in_946_cells_/1140572", "title"=>"Regulation of Ccnd2 expression by prolactin signaling in β-cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-08-15 02:54:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1637663"], "description"=>"<div><p>The postnatal proliferation and maturation of insulin-secreting pancreatic β-cells are critical for glucose metabolism and disease development in adults. Elucidation of the molecular mechanisms underlying these events will be beneficial to direct the differentiation of stem cells into functional β-cells. Maturation of β-cells is accompanied by increased expression of MafA, an insulin gene transcription factor. Transcriptome analysis of <i>MafA</i> knockout islets revealed MafA is required for the expression of several molecules critical for β-cell function, including <i>Glut2</i>, <i>ZnT8</i>, <i>Granuphilin</i>, <i>Vdr</i>, <i>Pcsk1</i> and <i>Urocortin 3</i>, as well as <i>Prolactin receptor</i> (<i>Prlr</i>) and its downstream target <i>Cyclin D2</i> (<i>Ccnd2</i>). Inhibition of <i>MafA</i> expression in mouse islets or β-cell lines resulted in reduced expression of Prlr and Ccnd2, and MafA transactivated the <i>Prlr</i> promoter. Stimulation of β-cells by prolactin resulted in the phosphorylation and translocation of Stat5B and an increased nuclear pool of Ccnd2 via Prlr and Jak2. Consistent with these results, the loss of MafA resulted in impaired proliferation of β-cells at 4 weeks of age. These results suggest that MafA regulates the postnatal proliferation of β-cells via prolactin signaling.</p></div>", "links"=>[], "tags"=>["target Cyclin D 2", "5b", "postnatal proliferation", "expression", "MafA knockout islets", "prlr", "insulin gene transcription factor"], "article_id"=>1140575, "categories"=>["Biological Sciences"], "users"=>["Koki Eto", "Wataru Nishimura", "Hisashi Oishi", "Haruhide Udagawa", "Miho Kawaguchi", "Masaki Hiramoto", "Toshiyoshi Fujiwara", "Satoru Takahashi", "Kazuki Yasuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0104184", "stats"=>{"downloads"=>25, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MafA_Is_Required_for_Postnatal_Proliferation_of_Pancreatic_946_Cells_/1140575", "title"=>"MafA Is Required for Postnatal Proliferation of Pancreatic β-Cells", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-08-15 02:54:31"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"21", "full-text"=>"18", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"56", "full-text"=>"43", "pdf"=>"30", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"44", "full-text"=>"35", "pdf"=>"25", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"36", "full-text"=>"34", "pdf"=>"20", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"23", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"27", "full-text"=>"19", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"6", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[285]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}, {"subject_area"=>"/Medicine and health sciences/Endocrinology", "average_usage"=>[282, 453]}]}
Loading … Spinner
There are currently no alerts