CiteULike

Mendeley | Further Information

{"title"=>"Differential effects of cold atmospheric plasma in the treatment of malignant glioma", "type"=>"journal", "authors"=>[{"first_name"=>"Alan", "last_name"=>"Siu", "scopus_author_id"=>"54409586500"}, {"first_name"=>"Olga", "last_name"=>"Volotskova", "scopus_author_id"=>"36128982600"}, {"first_name"=>"Xiaoqian", "last_name"=>"Cheng", "scopus_author_id"=>"56082801900"}, {"first_name"=>"Siri S.", "last_name"=>"Khalsa", "scopus_author_id"=>"57189688422"}, {"first_name"=>"Ka", "last_name"=>"Bian", "scopus_author_id"=>"51763144700"}, {"first_name"=>"Ferid", "last_name"=>"Murad", "scopus_author_id"=>"7102163579"}, {"first_name"=>"Michael", "last_name"=>"Keidar", "scopus_author_id"=>"7005101837"}, {"first_name"=>"Jonathan H.", "last_name"=>"Sherman", "scopus_author_id"=>"7201895450"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "doi"=>"10.1371/journal.pone.0126313", "sgr"=>"84939173216", "scopus"=>"2-s2.0-84939173216", "pmid"=>"26083405", "pui"=>"605585533"}, "id"=>"bed04de1-a977-3580-918b-8e7faf568f0a", "abstract"=>"OBJECTIVE: Cold atmospheric plasma (CAP) has recently been shown to selectively target cancer cells with minimal effects on normal cells. We systematically assessed the effects of CAP in the treatment of glioblastoma.\\n\\nMETHODS: Three glioma cell lines, normal astrocytes, and endothelial cell lines were treated with CAP. The effects of CAP were then characterized for viability, cytotoxicity/apoptosis, and cell cycle effects. Statistical significance was determined with student's t-test.\\n\\nRESULTS: CAP treatment decreases viability of glioma cells in a dose dependent manner, with the ID50 between 90-120 seconds for all glioma cell lines. Treatment with CAP for more than 120 seconds resulted in viability less than 35% at 24-hours posttreatment, with a steady decline to less than 20% at 72-hours. In contrast, the effect of CAP on the viability of NHA and HUVEC was minimal, and importantly not significant at 90 to 120 seconds, with up to 85% of the cells remained viable at 72-hours post-treatment. CAP treatment produces both cytotoxic and apoptotic effects with some variability between cell lines. CAP treatment resulted in a G2/M-phase cell cycle pause in all three cell lines.\\n\\nCONCLUSIONS: This preliminary study determined a multi-focal effect of CAP on glioma cells in vitro, which was not observed in the non-tumor cell lines. The decreased viability depended on the treatment duration and cell line, but overall was explained by the induction of cytotoxicity, apoptosis, and G2/M pause. Future studies will aim at further characterization with more complex pre-clinical models.", "link"=>"http://www.mendeley.com/research/differential-effects-cold-atmospheric-plasma-treatment-malignant-glioma", "reader_count"=>36, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>7, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>9, "Other"=>2, "Student > Master"=>2, "Student > Bachelor"=>8, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>7, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>9, "Other"=>2, "Student > Master"=>2, "Student > Bachelor"=>8, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>9, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>6, "Materials Science"=>1, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>3, "Physics and Astronomy"=>5, "Chemistry"=>5, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>9}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>5}, "Physics and Astronomy"=>{"Physics and Astronomy"=>5}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>4}

ORCID

CrossRef

• http://doi.org/10.1038/srep29098
• http://doi.org/10.1088/0022-3727/49/27/273001
• http://doi.org/10.1021/acsami.9b10620
• http://doi.org/10.3390/cancers11121920
• http://doi.org/10.1002/term.2224
• http://doi.org/10.1007/s13277-016-4911-7
• http://doi.org/10.1186/s12935-019-0856-4
• http://doi.org/10.1002/ppap.201500140
• http://doi.org/10.1063/1.5060650
• http://doi.org/10.1007/s00105-015-3686-x
• http://doi.org/10.1038/s41598-017-13041-8
• http://doi.org/10.3390/cancers12010245
• http://doi.org/10.1038/s41598-018-35166-0
• http://doi.org/10.1109/TRPMS.2017.2766027
• http://doi.org/10.12968/jowc.2017.26.8.470
• http://doi.org/10.1038/s41598-019-47128-1
• http://doi.org/10.1038/s41598-019-49013-3
• http://doi.org/10.1007/s11090-018-9910-6
• http://doi.org/10.1038/s41598-019-50329-3
• http://doi.org/10.1007/s12325-016-0338-1
• http://doi.org/10.1016/j.freeradbiomed.2017.11.011
• http://doi.org/10.3390/cancers11081109
• http://doi.org/10.1038/bjc.2016.12
• http://doi.org/10.1088/2516-1067/ab4ea9
• http://doi.org/10.1007/s11090-017-9845-3

DataCite

Europe PMC Citations

Europe PMC Database Citations

PubMed Central

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84939173216"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84939173216?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84939173216&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84939173216&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84939173216", "dc:identifier"=>"SCOPUS_ID:84939173216", "eid"=>"2-s2.0-84939173216", "dc:title"=>"Differential effects of cold atmospheric plasma in the treatment of malignant glioma", "dc:creator"=>"Siu A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"6", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-06-17", "prism:coverDisplayDate"=>"17 June 2015", "prism:doi"=>"10.1371/journal.pone.0126313", "citedby-count"=>"33", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"The George Washington University", "affiliation-city"=>"Washington, D.C.", "affiliation-country"=>"United States"}], "pubmed-id"=>"26083405", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0126313", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Web of Science® | Further Information

Article Coverage

Article Coverage Curated

Facebook

• {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0126313", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Journal Comments

Reddit

Twitter

Wikipedia

Counter

• {"month"=>"6", "year"=>"2015", "pdf_views"=>"43", "xml_views"=>"5", "html_views"=>"98"}
• {"month"=>"7", "year"=>"2015", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"63"}
• {"month"=>"8", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"34"}
• {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"24"}
• {"month"=>"10", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"45"}
• {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"14"}
• {"month"=>"12", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
• {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
• {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
• {"month"=>"3", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"43"}
• {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"45"}
• {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
• {"month"=>"6", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"46"}
• {"month"=>"7", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"44"}
• {"month"=>"8", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
• {"month"=>"9", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"34"}
• {"month"=>"10", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"31"}
• {"month"=>"11", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
• {"month"=>"12", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"35"}
• {"month"=>"1", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"27"}
• {"month"=>"2", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"52"}
• {"month"=>"3", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
• {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
• {"month"=>"5", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"41"}
• {"month"=>"6", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"35"}
• {"month"=>"7", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"31"}
• {"month"=>"8", "year"=>"2017", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"41"}
• {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"32"}
• {"month"=>"10", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"53"}
• {"month"=>"11", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"77"}
• {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"97"}
• {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"29"}
• {"month"=>"2", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
• {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
• {"month"=>"4", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"25"}
• {"month"=>"5", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"18"}
• {"month"=>"6", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
• {"month"=>"7", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"16"}
• {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"35"}
• {"month"=>"9", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"27"}
• {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"16"}
• {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"23"}
• {"month"=>"12", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"21"}
• {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"16"}
• {"month"=>"2", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"16"}
• {"month"=>"3", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
• {"month"=>"4", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
• {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"22"}
• {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"9"}
• {"month"=>"7", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"29"}
• {"month"=>"8", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"19"}
• {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
• {"month"=>"10", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
• {"month"=>"11", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"13"}
• {"month"=>"12", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"20"}
• {"month"=>"1", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"16"}
• {"month"=>"2", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"8"}

Figshare

• {"files"=>["https://ndownloader.figshare.com/files/2120583"], "description"=>"<p>(A) Cold atmospheric plasma (CAP) jet, with which can be mounted for treatment directly over culture plate containing various glioma cell lines (U87, U373, A172) for various durations. (B) The parameters of the CAP were an output voltage between 4.5–5 kV, a frequency of 13 kHz and a helium flow rate at 10 L/min. The distance between the jet and the base of the culture plate was maintained at 20 mm, yielding an average jet dose of 0.8 J/sec/cm².</p>", "links"=>[], "tags"=>["target cancer cells", "glioblastoma.MethodsThree glioma cell lines", "CAP treatment", "Cell lines", "Cold Atmospheric Plasma", "120 seconds", "viability", "cell cycle effects", "glioma cells", "Malignant Glioma ObjectiveCold", "huvec", "glioma cell lines", "nha", "id"], "article_id"=>1452468, "categories"=>["Biological Sciences"], "users"=>["Alan Siu", "Olga Volotskova", "Xiaoqian Cheng", "Siri S. Khalsa", "Ka Bian", "Ferid Murad", "Michael Keidar", "Jonathan H. Sherman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126313.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cold_Atmospheric_Plasma_CAP_jet_setup_/1452468", "title"=>"Cold Atmospheric Plasma (CAP) jet setup.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-17 04:31:10"}
• {"files"=>["https://ndownloader.figshare.com/files/2120584"], "description"=>"<p>Spectra of reactive species generated by CAP on media (DMEM, 10% FBS, 1% abx), which produces a stable proportion of reactive species over the duration of treatment (30 to 180 seconds).</p>", "links"=>[], "tags"=>["target cancer cells", "glioblastoma.MethodsThree glioma cell lines", "CAP treatment", "Cell lines", "Cold Atmospheric Plasma", "120 seconds", "viability", "cell cycle effects", "glioma cells", "Malignant Glioma ObjectiveCold", "huvec", "glioma cell lines", "nha", "id"], "article_id"=>1452469, "categories"=>["Biological Sciences"], "users"=>["Alan Siu", "Olga Volotskova", "Xiaoqian Cheng", "Siri S. Khalsa", "Ka Bian", "Ferid Murad", "Michael Keidar", "Jonathan H. Sherman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126313.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Spectral_Characterization_of_CAP_jet_/1452469", "title"=>"Spectral Characterization of CAP jet.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-17 04:31:10"}
• {"files"=>["https://ndownloader.figshare.com/files/2120585"], "description"=>"<p>Results of MTT assay from (A) A172, (B) U373, (C) U87, (D) NHA and (E) HUVEC treated with varying durations of cold atmospheric plasma (CAP). After treatment, the MTT assay was performed at 24, 48, and 72 hours. Viability was calculated relative to control. All three glioma cell lines exhibited a dose-dependent decline in viability with CAP treatments ranging from 60 to 180 seconds. The effects of CAP were sustained up to 72 hours post-treatment. Compared to the other two cancer cell lines, U87 seemed to be more resistant to CAP treatment for 90 seconds. No significant response was seen with NHA, and only transient effects observed on HUVEC cells. * denotes statistical significance compared to control (P <0.05).</p>", "links"=>[], "tags"=>["target cancer cells", "glioblastoma.MethodsThree glioma cell lines", "CAP treatment", "Cell lines", "Cold Atmospheric Plasma", "120 seconds", "viability", "cell cycle effects", "glioma cells", "Malignant Glioma ObjectiveCold", "huvec", "glioma cell lines", "nha", "id"], "article_id"=>1452470, "categories"=>["Biological Sciences"], "users"=>["Alan Siu", "Olga Volotskova", "Xiaoqian Cheng", "Siri S. Khalsa", "Ka Bian", "Ferid Murad", "Michael Keidar", "Jonathan H. Sherman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126313.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cell_viability_under_CAP_treatment_/1452470", "title"=>"Cell viability under CAP treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-17 04:31:10"}
• {"files"=>["https://ndownloader.figshare.com/files/2120586"], "description"=>"<p>Duration of CAP treatment necessary to decrease viability by 50%, termed the inhibitory duration (ID_<b>50</b>). The ID_<b>50</b> for (A) A172, (B) U373, and (C) U87 is between a treatment duration of 90 to 120 seconds with CAP. The ID_<b>50</b> for A172 and U373 declined at 48 and 72 hours more significantly than in U87, indicating a relative treatment resistance to CAP in U87. (D)The ID_<b>50</b> for all cell lines are between 90 to 120 seconds at 24 hours.</p>", "links"=>[], "tags"=>["target cancer cells", "glioblastoma.MethodsThree glioma cell lines", "CAP treatment", "Cell lines", "Cold Atmospheric Plasma", "120 seconds", "viability", "cell cycle effects", "glioma cells", "Malignant Glioma ObjectiveCold", "huvec", "glioma cell lines", "nha", "id"], "article_id"=>1452471, "categories"=>["Biological Sciences"], "users"=>["Alan Siu", "Olga Volotskova", "Xiaoqian Cheng", "Siri S. Khalsa", "Ka Bian", "Ferid Murad", "Michael Keidar", "Jonathan H. Sherman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126313.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibitory_dose_of_CAP_ID_50_/1452471", "title"=>"Inhibitory dose of CAP (ID₅₀).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-17 04:31:10"}
• {"files"=>["https://ndownloader.figshare.com/files/2120587"], "description"=>"<p>Briefly, cells were treated with CAP for 60, 90 or 120 seconds, incubated with bis-AAF-R110 at 4, 24 and 48 hours post-treatment for 30 minutes for 30 minutes, and emission quantified, as per protocol. bis-AAF-R110 cleavage calculated and plotted relative to untreated control cells. (A) Cytotoxicity was greatest for A172 24 hours post-treatment. (B) Cytotoxicity was greatest for U373 at 3 and 48 hours post-treatment. (C) CAP treatment did not produce a significant amount of cytotoxicity, as measured by bis-AAF-R110 cleavage.* denotes statistical significance compared to control (P <0.05).</p>", "links"=>[], "tags"=>["target cancer cells", "glioblastoma.MethodsThree glioma cell lines", "CAP treatment", "Cell lines", "Cold Atmospheric Plasma", "120 seconds", "viability", "cell cycle effects", "glioma cells", "Malignant Glioma ObjectiveCold", "huvec", "glioma cell lines", "nha", "id"], "article_id"=>1452472, "categories"=>["Biological Sciences"], "users"=>["Alan Siu", "Olga Volotskova", "Xiaoqian Cheng", "Siri S. Khalsa", "Ka Bian", "Ferid Murad", "Michael Keidar", "Jonathan H. Sherman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126313.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytotoxic_effects_of_CAP_treatment_in_three_different_glioma_cell_lines_/1452472", "title"=>"Cytotoxic effects of CAP treatment in three different glioma cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-17 04:31:10"}
• {"files"=>["https://ndownloader.figshare.com/files/2120588"], "description"=>"<p>Briefly, cells were treated with CAP for 60, 90 or 120 seconds, incubated with DEVD at 4, 24 and 48 hours post-treatment for 30 minutes, and emission quantified, as per protocol. DEVD cleavage calculated and plotted relative to untreated control cells. (A) Treatment with the lowest dosage of CAP, 60s, induced apoptosis in A172. Higher dosages induced cytotoxicity instead of apoptosis. (B) Apoptosis was significantly increased at 48 hours post-CAP at 120 seconds. At 4 and 24 hours post-CAP, a significant decline in caspase 3/7 activity was detected, likely representing a decline in total population due to the cytotoxic effects observed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0126313#pone.0126313.g003\" target=\"_blank\">Fig 3</a>. (C) No significant increase in caspase 3/7 activity was noted with CAP treatment of U87 cells.* denotes statistical significance compared to control (P <0.05).</p>", "links"=>[], "tags"=>["target cancer cells", "glioblastoma.MethodsThree glioma cell lines", "CAP treatment", "Cell lines", "Cold Atmospheric Plasma", "120 seconds", "viability", "cell cycle effects", "glioma cells", "Malignant Glioma ObjectiveCold", "huvec", "glioma cell lines", "nha", "id"], "article_id"=>1452473, "categories"=>["Biological Sciences"], "users"=>["Alan Siu", "Olga Volotskova", "Xiaoqian Cheng", "Siri S. Khalsa", "Ka Bian", "Ferid Murad", "Michael Keidar", "Jonathan H. Sherman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126313.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Apoptotic_effects_of_CAP_treatment_in_three_different_glioma_cell_lines_/1452473", "title"=>"Apoptotic effects of CAP treatment in three different glioma cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-17 04:31:10"}
• {"files"=>["https://ndownloader.figshare.com/files/2120590"], "description"=>"<p>Briefly, each of the glioma cells were treated with 90s CAP, and DNA content was measured at 24, 48, and 72 hours post-CAP treatment. In A172, CAP treatment for 90s resulted in a significant G2/M arrest at (B) 48 and (C) 72 hours, but not at (A) 24 hours. In U373, 90s CAP treatment resulted in a significant increase in the S-phase and G2/M population at (D) 24 hours and sustained at (E) 48 and (F) 72 hours. In U87, CAP treatment for 90s resulted in a significant increase in the G2/M population at (G) 24 and (H) 48 hours that diminished by (I) 72 hours. * denotes statistical significance compared to control (P <0.05).</p>", "links"=>[], "tags"=>["target cancer cells", "glioblastoma.MethodsThree glioma cell lines", "CAP treatment", "Cell lines", "Cold Atmospheric Plasma", "120 seconds", "viability", "cell cycle effects", "glioma cells", "Malignant Glioma ObjectiveCold", "huvec", "glioma cell lines", "nha", "id"], "article_id"=>1452475, "categories"=>["Biological Sciences"], "users"=>["Alan Siu", "Olga Volotskova", "Xiaoqian Cheng", "Siri S. Khalsa", "Ka Bian", "Ferid Murad", "Michael Keidar", "Jonathan H. Sherman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0126313.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Determination_of_the_cell_cycle_effect_induced_by_CAP_treatment_in_each_glioma_cell_line_/1452475", "title"=>"Determination of the cell cycle effect induced by CAP treatment in each glioma cell line.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-06-17 04:31:10"}

PMC Usage Stats | Further Information

• {"unique-ip"=>"19", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
• {"unique-ip"=>"24", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
• {"unique-ip"=>"25", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
• {"unique-ip"=>"27", "full-text"=>"26", "pdf"=>"24", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
• {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
• {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
• {"unique-ip"=>"25", "full-text"=>"14", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
• {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
• {"unique-ip"=>"13", "full-text"=>"9", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
• {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
• {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
• {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
• {"unique-ip"=>"22", "full-text"=>"21", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
• {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
• {"unique-ip"=>"25", "full-text"=>"34", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
• {"unique-ip"=>"33", "full-text"=>"39", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
• {"unique-ip"=>"54", "full-text"=>"55", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
• {"unique-ip"=>"32", "full-text"=>"33", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2016", "month"=>"12"}
• {"unique-ip"=>"29", "full-text"=>"31", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
• {"unique-ip"=>"28", "full-text"=>"30", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
• {"unique-ip"=>"30", "full-text"=>"31", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
• {"unique-ip"=>"20", "full-text"=>"22", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
• {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
• {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
• {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
• {"unique-ip"=>"21", "full-text"=>"26", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
• {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
• {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
• {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
• {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
• {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
• {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
• {"unique-ip"=>"21", "full-text"=>"27", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
• {"unique-ip"=>"26", "full-text"=>"29", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
• {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
• {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
• {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
• {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
• {"unique-ip"=>"23", "full-text"=>"27", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
• {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
• {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
• {"unique-ip"=>"21", "full-text"=>"19", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
• {"unique-ip"=>"12", "full-text"=>"20", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
• {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
• {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
• {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
• {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
• {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
• {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
• {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}

F1000Prime