Fluorescence In Situ Hybridization (FISH) Assays for Diagnosing Malaria in Endemic Areas
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{"title"=>"Fluorescence In Situ hybridization (FISH) assays for diagnosing malaria in endemic areas", "type"=>"journal", "authors"=>[{"first_name"=>"Jyotsna", "last_name"=>"Shah", "scopus_author_id"=>"7403266040"}, {"first_name"=>"Olivia", "last_name"=>"Mark", "scopus_author_id"=>"56971164900"}, {"first_name"=>"Helena", "last_name"=>"Weltman", "scopus_author_id"=>"6603372294"}, {"first_name"=>"Nicolas", "last_name"=>"Barcelo", "scopus_author_id"=>"57193858455"}, {"first_name"=>"Wai", "last_name"=>"Lo", "scopus_author_id"=>"56970533000"}, {"first_name"=>"Danuta", "last_name"=>"Wronska", "scopus_author_id"=>"57193339392"}, {"first_name"=>"Srinivas", "last_name"=>"Kakkilaya", "scopus_author_id"=>"6503891370"}, {"first_name"=>"Aravinda", "last_name"=>"Rao", "scopus_author_id"=>"56970268400"}, {"first_name"=>"Shalia T.", "last_name"=>"Bhat", "scopus_author_id"=>"56970548500"}, {"first_name"=>"Ruchi", "last_name"=>"Sinha", "scopus_author_id"=>"56970447400"}, {"first_name"=>"Sabah", "last_name"=>"Omar", "scopus_author_id"=>"7101977670"}, {"first_name"=>"Peter", "last_name"=>"O'bare", "scopus_author_id"=>"56971395800"}, {"first_name"=>"Manuel", "last_name"=>"Moro", "scopus_author_id"=>"7102336285"}, {"first_name"=>"Robert H.", "last_name"=>"Gilman", "scopus_author_id"=>"36044231400"}, {"first_name"=>"Nick", "last_name"=>"Harris", "scopus_author_id"=>"35450590300"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"26333092", "scopus"=>"2-s2.0-84947760071", "doi"=>"10.1371/journal.pone.0136726", "sgr"=>"84947760071", "pui"=>"606945868"}, "id"=>"d6e07cfc-ea81-3dc2-9a1f-b244829f6e47", "abstract"=>"Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field conditions, it still remains the gold standard for the diagnosis of malaria. Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in blood smears and describe the use of an LED light source that makes the method suitable for use in resource-limited malaria endemic countries. The Plasmodium Genus (P-Genus) FISH assay has a Plasmodium genus specific probe that detects all five species of Plasmodium known to cause the disease in humans. The P. falciparum (PF) FISH assay and P. vivax (PV) FISH assay detect and differentiate between P. falciparum and P. vivax respectively from other Plasmodium species. The FISH assays are more sensitive than Giemsa. The sensitivities of P-Genus, PF and PV FISH assays were found to be 98.2%, 94.5% and 98.3%, respectively compared to 89.9%, 83.3% and 87.9% for the detection of Plasmodium, P. falciparum and P. vivax by Giemsa staining respectively.", "link"=>"http://www.mendeley.com/research/fluorescence-situ-hybridization-fish-assays-diagnosing-malaria-endemic-areas-1", "reader_count"=>21, "reader_count_by_academic_status"=>{"Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Other"=>1, "Student > Master"=>5, "Student > Bachelor"=>4, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Other"=>1, "Student > Master"=>5, "Student > Bachelor"=>4, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Nursing and Health Professions"=>1, "Medicine and Dentistry"=>6, "Agricultural and Biological Sciences"=>7, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>6}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2251496"], "description"=>"<p>Blood smears from different <i>Plasmodium</i> species <i>P</i>. <i>falciparum</i>, <i>P</i>. <i>vivax</i>, <i>P</i>. <i>knowlesi</i>, <i>P</i>. <i>ovale and P</i>. <i>malariae</i> were analyzed using <i>Plasmodium</i> genus FISH assay. (1) All the processed smears were read with a 100X objective in a fluorescence microscope. (2) <i>P</i>. <i>falciparum</i>. <i>P</i>. <i>vivax</i>, <i>P</i>. <i>knowlesi</i> and negative control smears were read with a 100X objective on a regular microscope with a LED unit. Green fluorescence indicates the presence of <i>Plasmodium</i> ribosomal RNA (rRNA). (A) <i>P</i>. <i>falciparum</i> including crescent shaped gametocytes <i>(B) P</i>. <i>vivax</i>; (C) <i>P</i>. <i>knowlesi</i>; (D) <i>P</i>. <i>ovale</i>; (E) <i>P</i>. <i>malariae</i>; and (N) <i>Negative Control</i>; (A1) <i>P</i>. <i>falciparum</i>;. (B1) <i>P</i>. <i>vivax</i>; (C1) <i>P</i>. <i>knowlesi</i>; and (N1) <i>Negative Control</i>.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534242, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blood_smears_tested_with_Plasmodium_genus_FISH_assay_/1534242", "title"=>"Blood smears tested with <i>Plasmodium</i> genus FISH assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251498"], "description"=>"<p>Malaria positive patient blood samples from two collection sites, Peru (A), and Kenya (B) were analyzed using PF-FISH assay. (A) Patient blood positive for <i>P</i>. <i>falciparum</i>. (B) Patient blood positive for <i>P</i>. <i>malariae</i> [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136726#pone.0136726.ref001\" target=\"_blank\">1</a>] <i>and P</i>. <i>falciparum</i> gametocyte [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0136726#pone.0136726.ref002\" target=\"_blank\">2</a>]. (C) Patient blood positive for <i>P</i>. <i>ovale</i>. (D) Patient blood positive for <i>P</i>. <i>vivax</i>. Green fluorescence is due to the <i>P</i>. <i>falciparum</i> specific probe and red fluorescence due to the <i>Plasmodium</i> genus probe.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534244, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.g002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blood_smears_tested_with_P_falciparum_FISH_assay_/1534244", "title"=>"Blood smears tested with <i>P</i>. <i>falciparum</i> FISH assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251504"], "description"=>"<p>Malaria positive patient blood samples from two collection sites, Peru (A), India (B) and Kenya (C-E) were analyzed using PV-FISH assay. (A) Patient blood positive for <i>P</i>. <i>vivax</i>. (B) Patient blood positive for <i>P</i>. <i>vivax</i>. (C) Patient blood positive for <i>P</i>. <i>ovale</i>. (D) Patient blood positive for <i>P</i>. <i>malariae</i>. (E) Patient blood positive for <i>P</i>. <i>falciparum</i>. Green fluorescence is due to reactivity with the <i>P</i>. <i>vivax</i> specific probe and red fluorescence is due to reactivity with the <i>Plasmodium</i> genus probe.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534246, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.g003", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blood_smears_tested_with_P_vivax_FISH_assay_/1534246", "title"=>"Blood smears tested with <i>P</i>. <i>vivax</i> FISH assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251505"], "description"=>"<p>Blood smears prepared from <i>P</i>. <i>falciparum</i> positive patient before (0 Hrs) and after 24 hours (24Hrs) drug treatment were analyzed by <i>Plasmodium</i> Genus FISH assay. The disappearance of the <i>Plasmodium</i> Genus fluorescence signal at 24 hours suggests that FISH assay only detects live <i>Plasmodium</i> parasites.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534247, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.g004", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FISH_assay_only_detects_live_Plasmodium_parasites_/1534247", "title"=>"FISH assay only detects live <i>Plasmodium</i> parasites.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251509"], "description"=>"<p>Smear prepared from finger-prick capillary blood without anticoagulant. Green fluorescence indicates the presence of <i>Plasmodium</i> ribosomal RNA (rRNA) due to reaction with <i>Plasmodium</i> genus FISH.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534251, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.g005", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_P_falciparum_positive_patient_s_finger_prick_capillary_blood_smear_tested_with_Plasmodium_genus_FISH_assay_/1534251", "title"=>"<i>P</i>. <i>falciparum</i> positive patient’s finger-prick capillary blood smear tested with <i>Plasmodium</i> genus FISH assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251510"], "description"=>"<p>N/A—not applicable.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534252, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.t001", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Lowest_number_of_parasites_detected_per_956_l_blood_by_Giemsa_and_FISH_/1534252", "title"=>"Lowest number of parasites detected per μl blood by Giemsa and FISH.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251511"], "description"=>"<p>Giemsa negative, PCR and FISH Positive samples a = 17; b = 5; c = 14. (Note: 150 samples from US are excluded)</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534253, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.t002", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_Clinical_Study_Results_/1534253", "title"=>"Summary of Clinical Study Results.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251513"], "description"=>"<p>After discrepant analysis: Including FISH and PCR positive but Giemsa negative samples as true positives.” P-Genus FISH -17; PF-FISH– 5; and PV-FISH– 14. CI—confidence interval.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534255, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.t003", "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sensitivity_of_Plasmodium_FISH_tests_and_RDTs_as_compared_to_Giemsa_/1534255", "title"=>"Sensitivity of Plasmodium FISH tests and RDTs as compared to Giemsa.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251515"], "description"=>"<p>After discrepant analysis: Including FISH and PCR positive but Giemsa negative samples as true positives”. P-Genus FISH -17; PF-FISH– 5; and PV-FISH– 14. CI—confidence interval.</p>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534257, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0136726.t004", "stats"=>{"downloads"=>7, "page_views"=>35, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Specificity_of_Plasmodium_FISH_tests_and_RDTs_as_compared_to_Giemsa_/1534257", "title"=>"Specificity of Plasmodium FISH tests and RDTs as compared to Giemsa.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-09-02 03:56:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/2251517", "https://ndownloader.figshare.com/files/2251518"], "description"=>"<div><p>Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field conditions, it still remains the gold standard for the diagnosis of malaria. Here, we report the development of a fluorescence in situ hybridization (FISH) based method for detecting malaria infection in blood smears and describe the use of an LED light source that makes the method suitable for use in resource-limited malaria endemic countries. The <i>Plasmodium</i> Genus (P-Genus) FISH assay has a <i>Plasmodium</i> genus specific probe that detects all five species of <i>Plasmodium</i> known to cause the disease in humans. The <i>P</i>. <i>falciparum</i> (PF) FISH assay and <i>P</i>. <i>vivax</i> (PV) FISH assay detect and differentiate between <i>P</i>. <i>falciparum</i> and <i>P</i>. <i>vivax</i> respectively from other <i>Plasmodium</i> species. The FISH assays are more sensitive than Giemsa. The sensitivities of P-Genus, PF and PV FISH assays were found to be 98.2%, 94.5% and 98.3%, respectively compared to 89.9%, 83.3% and 87.9% for the detection of <i>Plasmodium</i>, <i>P</i>. <i>falciparum</i> and <i>P</i>. <i>vivax</i> by Giemsa staining respectively.</p></div>", "links"=>[], "tags"=>["PV FISH assays", "pf", "method", "plasmodium", "malaria", "FISH assay", "falciparum", "giemsa", "Endemic Areas Malaria", "vivax", "LED light source", "blood smears"], "article_id"=>1534259, "categories"=>["Uncategorised"], "users"=>["Jyotsna Shah", "Olivia Mark", "Helena Weltman", "Nicolas Barcelo", "Wai Lo", "Danuta Wronska", "Srinivas Kakkilaya", "Aravinda Rao", "Shalia T. Bhat", "Ruchi Sinha", "Sabah Omar", "Peter O’bare", "Manuel Moro", "Robert H. Gilman", "Nick Harris"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0136726.s001", "https://dx.doi.org/10.1371/journal.pone.0136726.s002"], "stats"=>{"downloads"=>13, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Fluorescence_In_Situ_Hybridization_FISH_Assays_for_Diagnosing_Malaria_in_Endemic_Areas/1534259", "title"=>"Fluorescence <i>In Situ</i> Hybridization (FISH) Assays for Diagnosing Malaria in Endemic Areas", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-09-02 03:56:53"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
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