Seasonal Dynamics in the Chemistry and Structure of the Fat Bodies of Bumblebee Queens
Publication Date
November 11, 2015
Journal
PLOS ONE
Authors
Alena Votavová, Aleš Tomčala, Edita Kofroňová, Michaela Kudzejová, et al
Volume
10
Issue
11
Pages
e0142261
DOI
https://dx.plos.org/10.1371/journal.pone.0142261
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0142261
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/26559946
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641598
Europe PMC
http://europepmc.org/abstract/MED/26559946
Scopus
84955470293
Mendeley
http://www.mendeley.com/research/seasonal-dynamics-chemistry-structure-fat-bodies-bumblebee-queens
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Seasonal dynamics in the chemistry and structure of the fat bodies of bumblebee queens", "type"=>"journal", "authors"=>[{"first_name"=>"Alena", "last_name"=>"Votavová", "scopus_author_id"=>"56325994100"}, {"first_name"=>"Aleš", "last_name"=>"Tomčala", "scopus_author_id"=>"23494056800"}, {"first_name"=>"Edita", "last_name"=>"Kofroňová", "scopus_author_id"=>"23980671400"}, {"first_name"=>"Michaela", "last_name"=>"Kudzejová", "scopus_author_id"=>"57076792000"}, {"first_name"=>"Jan", "last_name"=>"Šobotník", "scopus_author_id"=>"55879225000"}, {"first_name"=>"Pavel", "last_name"=>"Jiroš", "scopus_author_id"=>"9939413400"}, {"first_name"=>"Olga", "last_name"=>"Komzáková", "scopus_author_id"=>"35519906500"}, {"first_name"=>"Irena", "last_name"=>"Valterová", "scopus_author_id"=>"7004605546"}], "year"=>2015, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "doi"=>"10.1371/journal.pone.0142261", "sgr"=>"84955470293", "scopus"=>"2-s2.0-84955470293", "pmid"=>"26559946", "pui"=>"607878125"}, "id"=>"2160c4d2-31d5-383f-8bf5-35a179e1a749", "abstract"=>"Insects’ fat bodies are responsible for nutrient storage and for a significant part of intermediary metabolism. Thus, it can be expected that the structure and content of the fat body will adaptively change, if an insect is going through different life stages. Bumblebee queens belong to such insects as they dramatically change their physiology several times over their lives in relation to their solitary overwintering, independent colony foundation stage, and during the colony life-cycle ending in the senescent stage. Here, we report on changes in the ultrastructure and lipid composition of the peripheral fat body of Bombus terrestris queens in relation to seasonal changes in the queens’ activity. Six life stages are defined and evaluated in particular: pharate, callow, before and after hibernation, egg-laying, and senescence. Transmission electron microscopy revealed that the fat body contained two main cell types–adipocytes and oenocytes. Only adipocytes reveal important changes related to the life phase, and mostly the ration between inclusion and cytoplasm volume varies among particular stages. Both electron microscopy and chemical analyses of lipids highlighted seasonal variability in the quantity of the stored lipids, which peaked prior to hibernation. Triacylglycerols appeared to be the main energy source during hibernation, while the amount of glycogen before and after hibernation remained unchanged. In addition, we observed that the representation of some fatty acids within the triacylglycerols change during the queen’s life. Last but not least, we show that fat body cell membranes do not undergo substantial changes concerning phospholipid composition in relation to overwintering. This finding supports the hypothesis that the cold-adaptation strategy of bumblebee queens is more likely to be based on polyol accumulation than on the restructuring of lipid membranes.", "link"=>"http://www.mendeley.com/research/seasonal-dynamics-chemistry-structure-fat-bodies-bumblebee-queens", "reader_count"=>17, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>2, "Student > Bachelor"=>4, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>1, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>2, "Student > Master"=>2, "Student > Bachelor"=>4, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>10, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84955470293"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84955470293?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84955470293&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84955470293&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84955470293", "dc:identifier"=>"SCOPUS_ID:84955470293", "eid"=>"2-s2.0-84955470293", "dc:title"=>"Seasonal dynamics in the chemistry and structure of the fat bodies of bumblebee queens", "dc:creator"=>"Votavová A.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"10", "prism:issueIdentifier"=>"11", "prism:pageRange"=>nil, "prism:coverDate"=>"2015-11-01", "prism:coverDisplayDate"=>"1 November 2015", "prism:doi"=>"10.1371/journal.pone.0142261", "citedby-count"=>"10", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Research Institute for Fodder Crops, Ltd.", "affiliation-city"=>"Troubsko", "affiliation-country"=>"Czech Republic"}], "pubmed-id"=>"26559946", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0142261", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0142261", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"46", "xml_views"=>"8", "html_views"=>"293"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"33"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/2436346"], "description"=>"<p><b>(A) Trends in proportions of the most abundant triacylglycerols (TG).</b> Upper diagram shows the accumulation of particular TG in life stages involved in overwintering. Lower diagram shows a significant reduction in particular TG at the indicated life stages. <b>(B) Trends in proportions of saturated and monounsaturated fatty acids (FA) obtained by recalculation of HPLC/MS-APCI data.</b> Diagrams are focused on the most abundant FA, and some trends connected with overwintering are also observed. Generally, accumulation of monounsaturated FA and a decreased level of saturated FA in after- and before-hibernation life stages are obvious. Relative percentages were calculated from integrated peak areas of particular chromatographic peaks.</p>", "links"=>[], "tags"=>["body cell membranes", "hibernation", "colony foundation stage", "Bombus terrestris queens", "lipid", "transmission electron microscopy", "life stages"], "article_id"=>1599985, "categories"=>["Biological Sciences"], "users"=>["Alena Votavová", "Aleš Tomčala", "Edita Kofroňová", "Michaela Kudzejová", "Jan Šobotník", "Pavel Jiroš", "Olga Komzáková", "Irena Valterová"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0142261.g004", "stats"=>{"downloads"=>1, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Seasonal_Dynamics_in_the_Chemistry_and_Structure_of_the_Fat_Bodies_of_Bumblebee_Queens_Fig_4_/1599985", "title"=>"Seasonal Dynamics in the Chemistry and Structure of the Fat Bodies of Bumblebee Queens - Fig 4", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-11 02:59:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2436347"], "description"=>"<p>TG, triacylglycerols</p><p>Total lipid and TG weights in the FB of queens at different life stages (mean ± SD).</p>", "links"=>[], "tags"=>["body cell membranes", "hibernation", "colony foundation stage", "Bombus terrestris queens", "lipid", "transmission electron microscopy", "life stages"], "article_id"=>1599986, "categories"=>["Biological Sciences"], "users"=>["Alena Votavová", "Aleš Tomčala", "Edita Kofroňová", "Michaela Kudzejová", "Jan Šobotník", "Pavel Jiroš", "Olga Komzáková", "Irena Valterová"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0142261.t001", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Total_lipid_and_TG_weights_in_the_FB_of_queens_at_different_life_stages_mean_177_SD_/1599986", "title"=>"Total lipid and TG weights in the FB of queens at different life stages (mean ± SD).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2015-11-11 02:59:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2436349", "https://ndownloader.figshare.com/files/2436350", "https://ndownloader.figshare.com/files/2436351", "https://ndownloader.figshare.com/files/2436352", "https://ndownloader.figshare.com/files/2436353", "https://ndownloader.figshare.com/files/2436354", "https://ndownloader.figshare.com/files/2436355", "https://ndownloader.figshare.com/files/2436356", "https://ndownloader.figshare.com/files/2436357", "https://ndownloader.figshare.com/files/2436358", "https://ndownloader.figshare.com/files/2436359", "https://ndownloader.figshare.com/files/2436360"], "description"=>"<div><p>Insects’ fat bodies are responsible for nutrient storage and for a significant part of intermediary metabolism. Thus, it can be expected that the structure and content of the fat body will adaptively change, if an insect is going through different life stages. Bumblebee queens belong to such insects as they dramatically change their physiology several times over their lives in relation to their solitary overwintering, independent colony foundation stage, and during the colony life-cycle ending in the senescent stage. Here, we report on changes in the ultrastructure and lipid composition of the peripheral fat body of <i>Bombus terrestris</i> queens in relation to seasonal changes in the queens’ activity. Six life stages are defined and evaluated in particular: pharate, callow, before and after hibernation, egg-laying, and senescence. Transmission electron microscopy revealed that the fat body contained two main cell types–adipocytes and oenocytes. Only adipocytes reveal important changes related to the life phase, and mostly the ration between inclusion and cytoplasm volume varies among particular stages. Both electron microscopy and chemical analyses of lipids highlighted seasonal variability in the quantity of the stored lipids, which peaked prior to hibernation. Triacylglycerols appeared to be the main energy source during hibernation, while the amount of glycogen before and after hibernation remained unchanged. In addition, we observed that the representation of some fatty acids within the triacylglycerols change during the queen’s life. Last but not least, we show that fat body cell membranes do not undergo substantial changes concerning phospholipid composition in relation to overwintering. This finding supports the hypothesis that the cold-adaptation strategy of bumblebee queens is more likely to be based on polyol accumulation than on the restructuring of lipid membranes.</p></div>", "links"=>[], "tags"=>["body cell membranes", "hibernation", "colony foundation stage", "Bombus terrestris queens", "lipid", "transmission electron microscopy", "life stages"], "article_id"=>1599988, "categories"=>["Biological Sciences"], "users"=>["Alena Votavová", "Aleš Tomčala", "Edita Kofroňová", "Michaela Kudzejová", "Jan Šobotník", "Pavel Jiroš", "Olga Komzáková", "Irena Valterová"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0142261.s001", "https://dx.doi.org/10.1371/journal.pone.0142261.s002", "https://dx.doi.org/10.1371/journal.pone.0142261.s003", "https://dx.doi.org/10.1371/journal.pone.0142261.s004", "https://dx.doi.org/10.1371/journal.pone.0142261.s005", "https://dx.doi.org/10.1371/journal.pone.0142261.s006", "https://dx.doi.org/10.1371/journal.pone.0142261.s007", "https://dx.doi.org/10.1371/journal.pone.0142261.s008", "https://dx.doi.org/10.1371/journal.pone.0142261.s009", "https://dx.doi.org/10.1371/journal.pone.0142261.s010", "https://dx.doi.org/10.1371/journal.pone.0142261.s011", "https://dx.doi.org/10.1371/journal.pone.0142261.s012"], "stats"=>{"downloads"=>6, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Seasonal_Dynamics_in_the_Chemistry_and_Structure_of_the_Fat_Bodies_of_Bumblebee_Queens_/1599988", "title"=>"Seasonal Dynamics in the Chemistry and Structure of the Fat Bodies of Bumblebee Queens", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2015-11-11 02:59:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2436341"], "description"=>"<p>Scale bars represent 10 μm. Note the flat and highly condensed nucleus prior to hibernation and the initial phase of nucleus elimination in senescent queen. Abbreviations: cv, central vacuole; g, glycogen; ld, lipid droplet; m, mitochondria; n, nucleus; pg, protein granule; rer, rough endoplasmic reticulum.</p>", "links"=>[], "tags"=>["body cell membranes", "hibernation", "colony foundation stage", "Bombus terrestris queens", "lipid", "transmission electron microscopy", "life stages"], "article_id"=>1599980, "categories"=>["Biological Sciences"], "users"=>["Alena Votavová", "Aleš Tomčala", "Edita Kofroňová", "Michaela Kudzejová", "Jan Šobotník", "Pavel Jiroš", "Olga Komzáková", "Irena Valterová"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0142261.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ultrastructure_of_adipocytes_in_B_terrestris_queens_pharate_A_callow_B_before_hibernation_C_after_hibernation_D_egg_laying_E_and_senescent_F_/1599980", "title"=>"Ultrastructure of adipocytes in <i>B</i>. <i>terrestris</i> queens: pharate (A), callow (B), before hibernation (C), after hibernation (D), egg-laying (E), and senescent (F).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-11 02:59:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2436342"], "description"=>"<p>Comparison of cell volume and the proportions of glycogen, lipid droplets, and proteins at each life stage from transmission electron micrographs.</p>", "links"=>[], "tags"=>["body cell membranes", "hibernation", "colony foundation stage", "Bombus terrestris queens", "lipid", "transmission electron microscopy", "life stages"], "article_id"=>1599981, "categories"=>["Biological Sciences"], "users"=>["Alena Votavová", "Aleš Tomčala", "Edita Kofroňová", "Michaela Kudzejová", "Jan Šobotník", "Pavel Jiroš", "Olga Komzáková", "Irena Valterová"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0142261.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_cell_volume_and_the_proportions_of_glycogen_lipid_droplets_and_proteins_at_each_life_stage_from_transmission_electron_micrographs_/1599981", "title"=>"Comparison of cell volume and the proportions of glycogen, lipid droplets, and proteins at each life stage from transmission electron micrographs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-11 02:59:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/2436344"], "description"=>"<p>Adipocyte volume in μm<sup>3</sup>; lipid weight in mg; TG weight in mg.</p>", "links"=>[], "tags"=>["body cell membranes", "hibernation", "colony foundation stage", "Bombus terrestris queens", "lipid", "transmission electron microscopy", "life stages"], "article_id"=>1599983, "categories"=>["Biological Sciences"], "users"=>["Alena Votavová", "Aleš Tomčala", "Edita Kofroňová", "Michaela Kudzejová", "Jan Šobotník", "Pavel Jiroš", "Olga Komzáková", "Irena Valterová"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0142261.g003", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Trends_of_changes_in_adipocyte_size_total_lipid_weight_and_triacylglycerol_TG_weight_in_queens_at_different_life_stages_mean_values_cf_Table_1_and_S1_Table_/1599983", "title"=>"Trends of changes in adipocyte size, total lipid weight, and triacylglycerol (TG) weight in queens at different life stages (mean values, cf. Table 1 and S1 Table).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2015-11-11 02:59:05"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"6", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"24", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"6", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"19", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}

Relative Metric

{"start_date"=>"2015-01-01T00:00:00Z", "end_date"=>"2015-12-31T00:00:00Z", "subject_areas"=>[]}
Loading … Spinner
There are currently no alerts