ALM

Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations

Publication Date: August 29, 2016
Journal: PLOS ONE
Authors: Yan Wang, Myung Hee Kim, Seyed R. Tabaei, Jae Hyeok Park, et al
Volume: 11
Issue: 8
Pages: e0161915
DOI: https://dx.plos.org/10.1371/journal.pone.0161915
Publisher URL: http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0161915
Scopus: 84991501106
Mendeley: http://www.mendeley.com/research/spheroid-formation-hepatocarcinoma-cells-microwells-experiments-monte-carlo-simulations

Loading …

Events

Loading …

CiteULike

CiteULike

Mendeley 1315 May 23:18 UTC

Mendeley | Further Information

{"title"=>"Spheroid formation of hepatocarcinoma cells in microwells: Experiments and Monte Carlo simulations", "type"=>"journal", "authors"=>[{"first_name"=>"Yan", "last_name"=>"Wang", "scopus_author_id"=>"57191546209"}, {"first_name"=>"Myung Hee", "last_name"=>"Kim", "scopus_author_id"=>"56456754900"}, {"first_name"=>"Seyed R.", "last_name"=>"Tabaei", "scopus_author_id"=>"35182070900"}, {"first_name"=>"Jae Hyeok", "last_name"=>"Park", "scopus_author_id"=>"57191538096"}, {"first_name"=>"Kyuhwan", "last_name"=>"Na", "scopus_author_id"=>"37067827400"}, {"first_name"=>"Seok", "last_name"=>"Chung", "scopus_author_id"=>"7404292871"}, {"first_name"=>"Vladimir P.", "last_name"=>"Zhdanov", "scopus_author_id"=>"7402337180"}, {"first_name"=>"Nam Joon", "last_name"=>"Cho", "scopus_author_id"=>"16432736900"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"27571565", "scopus"=>"2-s2.0-84991501106", "pui"=>"612518309", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0161915", "sgr"=>"84991501106"}, "id"=>"256cb5e0-bd88-3850-bb01-7ef6ed3e851b", "abstract"=>"The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluores-cence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.", "link"=>"http://www.mendeley.com/research/spheroid-formation-hepatocarcinoma-cells-microwells-experiments-monte-carlo-simulations", "reader_count"=>12, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>2, "Neuroscience"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Neuroscience"=>{"Neuroscience"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Uruguay"=>1}, "group_count"=>1}

ORCID

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84991501106"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84991501106?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84991501106&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84991501106&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84991501106", "dc:identifier"=>"SCOPUS_ID:84991501106", "eid"=>"2-s2.0-84991501106", "dc:title"=>"Spheroid formation of hepatocarcinoma cells in microwells: Experiments and Monte Carlo simulations", "dc:creator"=>"Wang Y.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"11", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2016-08-01", "prism:coverDisplayDate"=>"August 2016", "prism:doi"=>"10.1371/journal.pone.0161915", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Centre for Biomimetic Sensor Science", "affiliation-city"=>"Singapore City", "affiliation-country"=>"Singapore"}, {"@_fa"=>"true", "affilname"=>"School of Materials Science and Engineering", "affiliation-city"=>"Singapore City", "affiliation-country"=>"Singapore"}], "pubmed-id"=>"27571565", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0161915", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Web of Science®13 Apr 04:43 UTC

{"id"=>"775807889482981376", "text"=>"#PLOSONE: Spheroid Formation of #Hepatocarcinoma Cells in #Microwells: Monte Carlo Simulations #cellline https://t.co/fDyNst17PY", "created_at"=>"2016-09-13T21:26:29Z", "user"=>"MaryRuberry", "user_name"=>"Mary Ruberry", "user_profile_image"=>"http://pbs.twimg.com/profile_images/628664741972590592/wKdSJapf_normal.jpg"}

Wikipedia16 Mar 21:43 UTC

{"month"=>"8", "year"=>"2016", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"304"}
{"month"=>"9", "year"=>"2016", "pdf_views"=>"89", "xml_views"=>"0", "html_views"=>"357"}
{"month"=>"10", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"56"}
{"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"53"}
{"month"=>"12", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"90"}
{"month"=>"1", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"44"}
{"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"40"}
{"month"=>"3", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"43"}
{"month"=>"4", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"49"}
{"month"=>"5", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"51"}
{"month"=>"6", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"37"}
{"month"=>"7", "year"=>"2017", "pdf_views"=>"24", "xml_views"=>"4", "html_views"=>"43"}
{"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"23"}
{"month"=>"9", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"39"}
{"month"=>"10", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"43"}
{"month"=>"11", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"138"}
{"month"=>"12", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"195"}
{"month"=>"1", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"39"}
{"month"=>"2", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"28"}
{"month"=>"3", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"18"}
{"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"14"}
{"month"=>"5", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"40"}
{"month"=>"6", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"12"}
{"month"=>"7", "year"=>"2018", "pdf_views"=>"29", "xml_views"=>"6", "html_views"=>"22"}
{"month"=>"8", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"33"}
{"month"=>"9", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"25"}
{"month"=>"10", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"32"}
{"month"=>"11", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
{"month"=>"12", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"20"}
{"month"=>"1", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"17"}
{"month"=>"2", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"16"}
{"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}
{"month"=>"4", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"39"}
{"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
{"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"27"}
{"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"30"}
{"month"=>"8", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"27"}
{"month"=>"9", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"24"}
{"month"=>"10", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"15"}
{"month"=>"11", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"23"}
{"month"=>"12", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"12"}
{"month"=>"1", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"11"}
{"month"=>"2", "year"=>"2020", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"11"}

Figshare

Figshare

PMC Usage Stats 25517 Feb 22:40 UTC

PMC Usage Stats

{"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
{"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
{"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
{"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
{"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
{"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
{"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
{"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
{"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
{"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
{"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
{"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
{"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
{"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
{"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
{"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
{"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
{"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
{"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
{"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
{"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
{"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
{"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
{"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
{"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
{"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
{"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
{"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
{"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
{"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
{"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
{"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
{"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
{"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
{"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
{"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

Relative Metric

F1000Prime19 Feb 13:47 UTC

F1000Prime

Loading …

There are currently no alerts

CiteULike

Mendeley | Further Information

ORCID

CrossRef

DataCite

Europe PMC Citations

Europe PMC Database Citations

PubMed Central

Scopus | Further Information

Web of Science®

Article Coverage

Article Coverage Curated

Facebook

Journal Comments

Nature

Reddit

Research Blogging

ScienceSeeker

Twitter

Wikipedia

Wordpress.com

Counter

Figshare

PMC Usage Stats

Relative Metric

F1000Prime