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Spheroid Formation of Hepatocarcinoma Cells in Microwells: Experiments and Monte Carlo Simulations

Publication Date: August 29, 2016
Journal: PLOS ONE
Authors: Yan Wang, Myung Hee Kim, Seyed R. Tabaei, Jae Hyeok Park, et al
Volume: 11
Issue: 8
Pages: e0161915
DOI: https://dx.plos.org/10.1371/journal.pone.0161915
Publisher URL: http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0161915
Scopus: 84991501106
Mendeley: http://www.mendeley.com/research/spheroid-formation-hepatocarcinoma-cells-microwells-experiments-monte-carlo-simulations

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Mendeley 1315 May 23:18 UTC

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{"title"=>"Spheroid formation of hepatocarcinoma cells in microwells: Experiments and Monte Carlo simulations", "type"=>"journal", "authors"=>[{"first_name"=>"Yan", "last_name"=>"Wang", "scopus_author_id"=>"57191546209"}, {"first_name"=>"Myung Hee", "last_name"=>"Kim", "scopus_author_id"=>"56456754900"}, {"first_name"=>"Seyed R.", "last_name"=>"Tabaei", "scopus_author_id"=>"35182070900"}, {"first_name"=>"Jae Hyeok", "last_name"=>"Park", "scopus_author_id"=>"57191538096"}, {"first_name"=>"Kyuhwan", "last_name"=>"Na", "scopus_author_id"=>"37067827400"}, {"first_name"=>"Seok", "last_name"=>"Chung", "scopus_author_id"=>"7404292871"}, {"first_name"=>"Vladimir P.", "last_name"=>"Zhdanov", "scopus_author_id"=>"7402337180"}, {"first_name"=>"Nam Joon", "last_name"=>"Cho", "scopus_author_id"=>"16432736900"}], "year"=>2016, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"27571565", "scopus"=>"2-s2.0-84991501106", "pui"=>"612518309", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic) 1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0161915", "sgr"=>"84991501106"}, "id"=>"256cb5e0-bd88-3850-bb01-7ef6ed3e851b", "abstract"=>"The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated low-adhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluores-cence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.", "link"=>"http://www.mendeley.com/research/spheroid-formation-hepatocarcinoma-cells-microwells-experiments-monte-carlo-simulations", "reader_count"=>12, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Master"=>1, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>2, "Neuroscience"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Neuroscience"=>{"Neuroscience"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Uruguay"=>1}, "group_count"=>1}

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Web of Science®13 Apr 04:43 UTC

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