Pathogen-specific deep sequence-coupled biopanning: A method for surveying human antibody responses
Publication Date
February 02, 2017
Journal
PLOS ONE
Authors
Kathryn M. Frietze, Juan M. Pascale, Brechla Moreno, Bryce Chackerian, et al
Volume
12
Issue
2
Pages
e0171511
DOI
https://dx.plos.org/10.1371/journal.pone.0171511
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0171511
Scopus
85011324234
Mendeley
http://www.mendeley.com/research/pathogenspecific-deep-sequencecoupled-biopanning-method-surveying-human-antibody-responses
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Mendeley | Further Information

{"title"=>"Pathogen-specific deep sequence-coupled biopanning: A method for surveying human antibody responses", "type"=>"journal", "authors"=>[{"first_name"=>"Kathryn M.", "last_name"=>"Frietze", "scopus_author_id"=>"36554129700"}, {"first_name"=>"Juan M.", "last_name"=>"Pascale", "scopus_author_id"=>"7004472331"}, {"first_name"=>"Brechla", "last_name"=>"Moreno", "scopus_author_id"=>"55311749700"}, {"first_name"=>"Bryce", "last_name"=>"Chackerian", "scopus_author_id"=>"6603169499"}, {"first_name"=>"David S.", "last_name"=>"Peabody", "scopus_author_id"=>"7006847757"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "sgr"=>"85011324234", "doi"=>"10.1371/journal.pone.0171511", "isbn"=>"1111111111", "pmid"=>"28152075", "scopus"=>"2-s2.0-85011324234", "pui"=>"614278761"}, "id"=>"ebae7f63-c64c-31da-baec-04901c672ae5", "abstract"=>"Identifying the targets of antibody responses during infection is important for designing vaccines, developing diagnostic and prognostic tools, and understanding pathogenesis. We developed a novel deep sequence-coupled biopanning approach capable of identifying the protein epitopes of antibodies present in human polyclonal serum. Here, we report the adaptation of this approach for the identification of pathogen-specific epitopes recognized by antibodies elicited during acute infection. As a proof-of-principle, we applied this approach to assessing antibodies to Dengue virus (DENV). Using a panel of sera from patients with acute secondary DENV infection, we panned a DENV antigen fragment library displayed on the surface of bacteriophage MS2 virus-like particles and characterized the population of affinity-selected peptide epitopes by deep sequence analysis. Although there was considerable variation in the responses of individuals, we found several epitopes within the Envelope glycoprotein and Non-Structural Protein 1 that were commonly enriched. This report establishes a novel approach for characterizing pathogen-specific antibody responses in human sera, and has future utility in identifying novel diagnostic and vaccine targets.", "link"=>"http://www.mendeley.com/research/pathogenspecific-deep-sequencecoupled-biopanning-method-surveying-human-antibody-responses", "reader_count"=>17, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>3}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>6, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>5, "Chemical Engineering"=>2, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}, "Chemical Engineering"=>{"Chemical Engineering"=>2}}, "reader_count_by_country"=>{"South Africa"=>1}, "group_count"=>2}

Scopus | Further Information

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