A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio
Publication Date
June 22, 2017
Authors
Govinda Rizal, Shanta Karki, Vivek Thakur, Samart Wanchana, et al
Volume
12
Issue
6
Pages
e0179567
DOI
https://dx.plos.org/10.1371/journal.pone.0179567
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0179567
Scopus
85021266576
Mendeley
http://www.mendeley.com/research/sorghum-sorghum-bicolor-mutant-altered-carbon-isotope-ratio
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Mendeley | Further Information

{"title"=>"A sorghum (Sorghum bicolor) mutant with altered carbon isotope ratio", "type"=>"journal", "authors"=>[{"first_name"=>"Govinda", "last_name"=>"Rizal", "scopus_author_id"=>"35070151800"}, {"first_name"=>"Shanta", "last_name"=>"Karki", "scopus_author_id"=>"54417403500"}, {"first_name"=>"Vivek", "last_name"=>"Thakur", "scopus_author_id"=>"15822924100"}, {"first_name"=>"Samart", "last_name"=>"Wanchana", "scopus_author_id"=>"24281989400"}, {"first_name"=>"Hugo", "last_name"=>"Alonso-Cantabrana", "scopus_author_id"=>"18435975200"}, {"first_name"=>"Jacque", "last_name"=>"Dionora", "scopus_author_id"=>"56897263800"}, {"first_name"=>"John E.", "last_name"=>"Sheehy", "scopus_author_id"=>"7006780452"}, {"first_name"=>"Robert", "last_name"=>"Furbank", "scopus_author_id"=>"7003488182"}, {"first_name"=>"Susanne", "last_name"=>"Von Caemmerer", "scopus_author_id"=>"7003449035"}, {"first_name"=>"William Paul", "last_name"=>"Quick", "scopus_author_id"=>"7005506933"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"616913679", "sgr"=>"85021266576", "pmid"=>"28640841", "scopus"=>"2-s2.0-85021266576", "doi"=>"10.1371/journal.pone.0179567", "issn"=>"19326203"}, "id"=>"806d3ba9-b44a-367b-b163-f13eac7035bb", "abstract"=>"Recent efforts to engineer C4 photosynthetic traits into C3 plants such as rice demand an understanding of the genetic elements that enable C4 plants to outperform C3 plants. As a part of the C4 Rice Consortium's efforts to identify genes needed to support C4 photosynthesis, EMS mutagenized sorghum populations were generated and screened to identify genes that cause a loss of C4 function. Stable carbon isotope ratio (delta13C) of leaf dry matter has been used to distinguishspecies with C3 and C4 photosynthetic pathways. Here, we report the identification of a sorghum (Sorghum bicolor) mutant with a low delta13C characteristic. A mutant (named Mut33) with a pale phenotype and stunted growth was identified from an EMS treated sorghum M2 population. The stable carbon isotope analysis of the mutants showed a decrease of 13C uptake capacity. The noise of random mutation was reduced by crossing the mutant and its wildtype (WT). The back-cross (BC1F1) progenies were like the WT parent in terms of 13C values and plant phenotypes. All the BC1F2 plants with low delta13C died before they produced their 6th leaf. Gas exchange measurements of the low delta13C sorghum mutants showed a higher CO2 compensation point (25.24 mumol CO2.mol-1air) and the maximum rate of photosynthesis was less than 5mumol.m-2.s-1. To identify the genetic determinant of this trait, four DNA pools were isolated; two each from normal and low delta13C BC1F2 mutant plants. These were sequenced using an Illumina platform. Comparison of allele frequency of the single nucleotide polymorphisms (SNPs) between the pools with contrasting phenotype showed that a locus in Chromosome 10 between 57,941,104 and 59,985,708 bps had an allele frequency of 1. There were 211 mutations and 37 genes in the locus, out of which mutations in 9 genes showed non-synonymous changes. This finding is expected to contribute to future research on the identification of the causal factor differentiating C4 from C3 species that can be used in the transformation of C3 to C4 plants.", "link"=>"http://www.mendeley.com/research/sorghum-sorghum-bicolor-mutant-altered-carbon-isotope-ratio", "reader_count"=>2, "reader_count_by_academic_status"=>{"Researcher"=>2}, "reader_count_by_user_role"=>{"Researcher"=>2}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>1, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}}, "group_count"=>0}

Scopus | Further Information

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