Whole genome microarray analysis of neural progenitor C17.2 cells during differentiation and validation of 30 neural mRNA biomarkers for estimation of developmental neurotoxicity
Publication Date
December 20, 2017
Authors
Kristina Attoff, Anda Gliga, Jessica Lundqvist, Ulf Norinder, et al
Volume
12
Issue
12
Pages
e0190066
DOI
https://dx.plos.org/10.1371/journal.pone.0190066
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0190066
Scopus
85038819307
Mendeley
http://www.mendeley.com/research/whole-genome-microarray-analysis-neural-progenitor-c172-cells-during-differentiation-validation-30-n
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Mendeley | Further Information

{"title"=>"Whole genome microarray analysis of neural progenitor C17.2 cells during differentiation and validation of 30 neural mRNA biomarkers for estimation of developmental neurotoxicity", "type"=>"journal", "authors"=>[{"first_name"=>"Kristina", "last_name"=>"Attoff", "scopus_author_id"=>"57189591282"}, {"first_name"=>"Anda", "last_name"=>"Gliga", "scopus_author_id"=>"56033828300"}, {"first_name"=>"Jessica", "last_name"=>"Lundqvist", "scopus_author_id"=>"35388748700"}, {"first_name"=>"Ulf", "last_name"=>"Norinder", "scopus_author_id"=>"7003382744"}, {"first_name"=>"Anna", "last_name"=>"Forsby", "scopus_author_id"=>"6701324942"}], "year"=>2017, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-85038819307", "sgr"=>"85038819307", "pui"=>"619853309", "isbn"=>"1111111111", "doi"=>"10.1371/journal.pone.0190066"}, "id"=>"c23fb890-bba9-3073-807f-d1691e4d0df1", "abstract"=>"Copyright: © 2017 Attoff et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Despite its high relevance, developmental neurotoxicity (DNT) is one of the least studied forms of toxicity. Current guidelines for DNT testing are based on in vivo testing and they require extensive resources. Transcriptomic approaches using relevant in vitro models have been suggested as a useful tool for identifying possible DNT-generating compounds. In this study, we performed whole genome microarray analysis on the murine progenitor cell line C17.2 following 5 and 10 days of differentiation. We identified 30 genes that are strongly associated with neural differentiation. The C17.2 cell line can be differentiated into a co-culture of both neurons and neuroglial cells, giving a more relevant picture of the brain than using neuronal cells alone. Among the most highly upregulated genes were genes involved in neurogenesis (CHRDL1), axonal guidance (BMP4), neuronal connectivity (PLXDC2), axonogenesis (RTN4R) and astrocyte differentiation (S100B). The 30 biomarkers were further validated by exposure to non-cytotoxic concentrations of two DNT-inducing compounds (valproic acid and methylmercury) and one neurotoxic chemical possessing a possible DNT activity (acrylamide). Twenty-eight of the 30 biomarkers were altered by at least one of the neurotoxic substances, proving the importance of these biomarkers during differentiation. These results suggest that gene expression profiling using a predefined set of biomarkers could be used as a sensitive tool for initial DNT screening of chemicals. Using a predefined set of mRNA biomarkers, instead of the whole genome, makes this model affordable and high-throughput. The use of such models could help speed up the initial screening of substances, possibly indicating alerts that need to be further studied in more sophisticated models.", "link"=>"http://www.mendeley.com/research/whole-genome-microarray-analysis-neural-progenitor-c172-cells-during-differentiation-validation-30-n", "reader_count"=>6, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>1, "Student > Doctoral Student"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>1, "Student > Doctoral Student"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Agricultural and Biological Sciences"=>1, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>1}, "reader_count_by_subdiscipline"=>{"Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "group_count"=>0}

Scopus | Further Information

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