Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation
Publication Date
April 17, 2018
Authors
Inger Greve Alsos, Youri Lammers, Nigel Giles Yoccoz, Tina Jørgensen, et al
Volume
13
Issue
4
Pages
e0195403
DOI
https://dx.plos.org/10.1371/journal.pone.0195403
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0195403
Scopus
85045618312
Mendeley
http://www.mendeley.com/research/plant-dna-metabarcoding-lake-sediments-it-represent-contemporary-vegetation
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation", "type"=>"journal", "authors"=>[{"first_name"=>"Inger Greve", "last_name"=>"Alsos"}, {"first_name"=>"Youri", "last_name"=>"Lammers"}, {"first_name"=>"Nigel Giles", "last_name"=>"Yoccoz"}, {"first_name"=>"Tina", "last_name"=>"Jørgensen"}, {"first_name"=>"Per", "last_name"=>"Sjögren"}, {"first_name"=>"Ludovic", "last_name"=>"Gielly"}, {"first_name"=>"Mary E.", "last_name"=>"Edwards"}], "year"=>2018, "source"=>"PLOS ONE", "identifiers"=>{"pui"=>"621689328", "doi"=>"10.1371/journal.pone.0195403", "issn"=>"1932-6203", "scopus"=>"2-s2.0-85045618312", "sgr"=>"85045618312", "isbn"=>"1111111111"}, "id"=>"4641a75a-954b-3e8c-a078-5ce322c30e15", "abstract"=>"Metabarcoding of lake sediments have been shown to reveal current and past biodiversity, but little is known about the degree to which taxa growing in the vegetation are represented in environmental DNA (eDNA) records. We analysed composition of lake and catchment vegetation and vascular plant eDNA at 11 lakes in northern Norway. Out of 489 records of taxa growing within 2 m from the lake shore, 17–49% (mean 31%) of the identifiable taxa recorded were detected with eDNA. Of the 217 eDNA records of 47 plant taxa in the 11 lakes, 73% and 12% matched taxa recorded in vegetation surveys within 2 m and up to about 50 m away from the lakeshore, respectively, whereas 16% were not recorded in the vegetation surveys of the same lake. The latter include taxa likely overlooked in the vegetation surveys or growing outside the survey area. The percentages detected were 61, 47, 25, and 15 for dominant, common, scattered, and rare taxa, respectively. Similar numbers for aquatic plants were 88, 88, 33 and 62%, respectively. Detection rate and taxonomic resolution varied among plant families and functional groups with good detection of e.g. Ericaceae, Roseaceae, deciduous trees, ferns, club mosses and aquatics. The representation of terrestrial taxa in eDNA depends on both their distance from the sampling site and their abundance and is sufficient for recording vegetation types. For aquatic vegetation, eDNA may be comparable with, or even superior to, in-lake vegetation surveys and may therefore be used as an tool for biomonitoring. For reconstruction of terrestrial vegetation, technical improvements and more intensive sampling is needed to detect a higher proportion of rare taxa although DNA of some taxa may never reach the lake sediments due to taphonomical constrains. Nevertheless, eDNA performs similar to conventional methods of pollen and macrofossil analyses and may therefore be an important tool for reconstruction of past vegetation.", "link"=>"http://www.mendeley.com/research/plant-dna-metabarcoding-lake-sediments-it-represent-contemporary-vegetation", "reader_count"=>22, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>3, "Lecturer"=>2, "Unspecified"=>1, "Student > Master"=>3, "Researcher"=>10, "Professor"=>1, "Student > Bachelor"=>1, "Student > Ph. D. Student"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>3, "Lecturer"=>2, "Unspecified"=>1, "Student > Master"=>3, "Researcher"=>10, "Professor"=>1, "Student > Bachelor"=>1, "Student > Ph. D. Student"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>13, "Environmental Science"=>4, "Unspecified"=>2}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Environmental Science"=>{"Environmental Science"=>4}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85045618312"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/85045618312?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85045618312&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85045618312&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/85045618312", "dc:identifier"=>"SCOPUS_ID:85045618312", "eid"=>"2-s2.0-85045618312", "dc:title"=>"Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation", "dc:creator"=>"Alsos I.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"13", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2018-04-01", "prism:coverDisplayDate"=>"April 2018", "prism:doi"=>"10.1371/journal.pone.0195403", "citedby-count"=>"11", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"UiT The Arctic University of Norway", "affiliation-city"=>"Tromso", "affiliation-country"=>"Norway"}], "pubmed-id"=>"29664954", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e0195403", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Twitter

Counter

  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"96", "xml_views"=>"7", "html_views"=>"164"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"99", "xml_views"=>"4", "html_views"=>"150"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"40", "xml_views"=>"6", "html_views"=>"69"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"40", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"57", "xml_views"=>"1", "html_views"=>"70"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"57", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"56", "xml_views"=>"1", "html_views"=>"88"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"69", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"49", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"63", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"59", "xml_views"=>"1", "html_views"=>"68"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"55", "xml_views"=>"2", "html_views"=>"67"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"46", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"33", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"51", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"53", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"27", "xml_views"=>"1", "html_views"=>"24"}

PMC Usage Stats

  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"18", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"56", "full-text"=>"44", "pdf"=>"19", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"13", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"30", "full-text"=>"36", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"18", "full-text"=>"23", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
Loading … Spinner
There are currently no alerts