Super-Resolution Microscopy Reveals Specific Recruitment of HIV-1 Envelope Proteins to Viral Assembly Sites Dependent on the Envelope C-Terminal Tail
- Publication Date
- February 28, 2013
- Journal
- PLOS Pathogens
- Authors
- Walter Muranyi, Sebastian Malkusch, Barbara Müller, Mike Heilemann, et al
- Volume
- 9
- Issue
- 2
- Pages
- e1003198
- DOI
- https://dx.plos.org/10.1371/journal.ppat.1003198
- Publisher URL
- http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1003198
- PubMed
- http://www.ncbi.nlm.nih.gov/pubmed/23468635
- PubMed Central
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585150
- Europe PMC
- http://europepmc.org/abstract/MED/23468635
- Web of Science
- 000321863200017
- Scopus
- 84878279566
- Mendeley
- http://www.mendeley.com/research/superresolution-microscopy-reveals-specific-recruitment-hiv1-envelope-proteins-viral-assembly-sites
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Mendeley | Further Information
{"title"=>"Super-Resolution Microscopy Reveals Specific Recruitment of HIV-1 Envelope Proteins to Viral Assembly Sites Dependent on the Envelope C-Terminal Tail", "type"=>"journal", "authors"=>[{"first_name"=>"Walter", "last_name"=>"Muranyi", "scopus_author_id"=>"6603637980"}, {"first_name"=>"Sebastian", "last_name"=>"Malkusch", "scopus_author_id"=>"37113717200"}, {"first_name"=>"Barbara", "last_name"=>"Müller", "scopus_author_id"=>"7403187010"}, {"first_name"=>"Mike", "last_name"=>"Heilemann", "scopus_author_id"=>"6701502015"}, {"first_name"=>"Hans Georg", "last_name"=>"Kräusslich", "scopus_author_id"=>"7006112877"}], "year"=>2013, "source"=>"PLoS Pathogens", "identifiers"=>{"pui"=>"369459545", "issn"=>"15537366", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "doi"=>"10.1371/journal.ppat.1003198", "scopus"=>"2-s2.0-84878279566", "pmid"=>"23468635", "sgr"=>"84878279566"}, "id"=>"c8f554b1-b869-39bc-9b9e-6928b75e4b6a", "abstract"=>"The inner structural Gag proteins and the envelope (Env) glycoproteins of human immunodeficiency virus (HIV-1) traffic independently to the plasma membrane, where they assemble the nascent virion. HIV-1 carries a relatively low number of glycoproteins in its membrane, and the mechanism of Env recruitment and virus incorporation is incompletely understood. We employed dual-color super-resolution microscopy visualizing Gag assembly sites and HIV-1 Env proteins in virus-producing and in Env expressing cells. Distinctive HIV-1 Gag assembly sites were readily detected and were associated with Env clusters that always extended beyond the actual Gag assembly site and often showed enrichment at the periphery and surrounding the assembly site. Formation of these Env clusters depended on the presence of other HIV-1 proteins and on the long cytoplasmic tail (CT) of Env. CT deletion, a matrix mutation affecting Env incorporation or Env expression in the absence of other HIV-1 proteins led to much smaller Env clusters, which were not enriched at viral assembly sites. These results show that Env is recruited to HIV-1 assembly sites in a CT-dependent manner, while Env(ΔCT) appears to be randomly incorporated. The observed Env accumulation surrounding Gag assemblies, with a lower density on the actual bud, could facilitate viral spread in vivo. Keeping Env molecules on the nascent virus low may be important for escape from the humoral immune response, while cell-cell contacts mediated by surrounding Env molecules could promote HIV-1 transmission through the virological synapse.", "link"=>"http://www.mendeley.com/research/superresolution-microscopy-reveals-specific-recruitment-hiv1-envelope-proteins-viral-assembly-sites", "reader_count"=>93, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>7, "Researcher"=>28, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>27, "Student > Postgraduate"=>6, "Student > Master"=>10, "Other"=>3, "Student > Bachelor"=>4, "Professor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>7, "Researcher"=>28, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>27, "Student > Postgraduate"=>6, "Student > Master"=>10, "Other"=>3, "Student > Bachelor"=>4, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>6, "Biochemistry, Genetics and Molecular Biology"=>14, "Agricultural and Biological Sciences"=>46, "Medicine and Dentistry"=>3, "Neuroscience"=>1, "Physics and Astronomy"=>12, "Chemistry"=>4, "Immunology and Microbiology"=>4}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>6}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>4}, "Physics and Astronomy"=>{"Physics and Astronomy"=>12}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>46}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>14}, "Unspecified"=>{"Unspecified"=>3}}, "reader_count_by_country"=>{"Czech Republic"=>1, "United States"=>2, "United Kingdom"=>1, "France"=>1, "Switzerland"=>1, "Germany"=>1}, "group_count"=>2}CrossRef
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Scopus | Further Information
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Relative Metric
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