Role of the Vibrio cholerae Matrix Protein Bap1 in Cross-Resistance to Antimicrobial Peptides
Publication Date
October 03, 2013
Journal
PLOS Pathogens
Authors
Marylise Duperthuy, Annika E. Sjöström, Dharmesh Sabharwal, Fatemeh Damghani, et al
Volume
9
Issue
10
Pages
e1003620
DOI
https://dx.plos.org/10.1371/journal.ppat.1003620
Publisher URL
http://journals.plos.org/plospathogens/article?id=10.1371%2Fjournal.ppat.1003620
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24098113
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789753
Europe PMC
http://europepmc.org/abstract/MED/24098113
Web of Science
000330383800011
Scopus
84887315028
Mendeley
http://www.mendeley.com/research/role-vibrio-cholerae-matrix-protein-bap1-crossresistance-antimicrobial-peptides
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Mendeley | Further Information

{"title"=>"Role of the Vibrio cholerae Matrix Protein Bap1 in Cross-Resistance to Antimicrobial Peptides", "type"=>"journal", "authors"=>[{"first_name"=>"Marylise", "last_name"=>"Duperthuy", "scopus_author_id"=>"36172536400"}, {"first_name"=>"Annika E.", "last_name"=>"Sjöström", "scopus_author_id"=>"9732378600"}, {"first_name"=>"Dharmesh", "last_name"=>"Sabharwal", "scopus_author_id"=>"36673734900"}, {"first_name"=>"Fatemeh", "last_name"=>"Damghani", "scopus_author_id"=>"55920880100"}, {"first_name"=>"Bernt Eric", "last_name"=>"Uhlin", "scopus_author_id"=>"7006211117"}, {"first_name"=>"Sun Nyunt", "last_name"=>"Wai", "scopus_author_id"=>"7004197354"}], "year"=>2013, "source"=>"PLoS Pathogens", "identifiers"=>{"scopus"=>"2-s2.0-84887315028", "doi"=>"10.1371/journal.ppat.1003620", "sgr"=>"84887315028", "isbn"=>"1553-7374 (Electronic)\\r1553-7366 (Linking)", "pmid"=>"24098113", "issn"=>"15537366", "pui"=>"370218032"}, "id"=>"2917d9fe-4c15-3e31-b128-93e7841065d3", "abstract"=>"Outer membrane vesicles (OMVs) that are released from Gram-negative pathogenic bacteria can serve as vehicles for the translocation of effectors involved in infectious processes. In this study we have investigated the role of OMVs of the Vibrio cholerae O1 El Tor A1552 strain in resistance to antimicrobial peptides (AMPs). To assess this potential role, we grew V. cholerae with sub-lethal concentrations of Polymyxin B (PmB) or the AMP LL-37 and analyzed the OMVs produced and their effects on AMP resistance. Our results show that growing V. cholerae in the presence of AMPs modifies the protein content of the OMVs. In the presence of PmB, bacteria release OMVs that are larger in size and contain a biofilm-associated extracellular matrix protein (Bap1). We demonstrated that Bap1 binds to the OmpT porin on the OMVs through the LDV domain of OmpT. In addition, OMVs from cultures incubated in presence of PmB also provide better protection for V. cholerae against LL-37 compared to OMVs from V. cholerae cultures grown without AMPs or in presence of LL-37. Using a bap1 mutant we showed that cross-resistance between PmB and LL-37 involved the Bap1 protein, whereby Bap1 on OMVs traps LL-37 with no subsequent degradation of the AMP.", "link"=>"http://www.mendeley.com/research/role-vibrio-cholerae-matrix-protein-bap1-crossresistance-antimicrobial-peptides", "reader_count"=>54, "reader_count_by_academic_status"=>{"Researcher"=>15, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>15, "Student > Postgraduate"=>2, "Student > Master"=>10, "Other"=>1, "Student > Bachelor"=>4, "Professor"=>2}, "reader_count_by_user_role"=>{"Researcher"=>15, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>15, "Student > Postgraduate"=>2, "Student > Master"=>10, "Other"=>1, "Student > Bachelor"=>4, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Unspecified"=>2, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>10, "Agricultural and Biological Sciences"=>27, "Medicine and Dentistry"=>1, "Physics and Astronomy"=>1, "Chemistry"=>2, "Immunology and Microbiology"=>5, "Earth and Planetary Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>5}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>27}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>2}

CrossRef

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1223625"], "description"=>"<p>SDS-PAGE (A), immunoblot analyses using anti-OmpU (B) and anti-Bap1 antiserum (C) and quantification of OMV yield by analyzing LPS content by ELISA (D) of OMVs samples from control A1552 cultures (lane 1) or A1552 cultures with a sub-lethal concentration of LL-37 (lane 2) or PmB (lane 3).</p>", "links"=>[], "tags"=>["omvs", "amp", "treated"], "article_id"=>813858, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analyses_of_OMVs_from_AMP_treated_V_cholerae_/813858", "title"=>"Analyses of OMVs from AMP treated <i>V. cholerae</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223628"], "description"=>"<p>Protective effect against LL-37 of OMVs from wild type A1552 grown with and without AMPs (A) and the Δ<i>bap1</i> mutant (B). Results are shown in µg/ml and represent the MIC values for LL-37 on A1552 measured after 1 h incubation of LL-37 with OMVs from A1552 grown in presence or absence of AMPs, followed by 16 h incubation with A1552 bacteria.</p>", "links"=>[], "tags"=>["amp-protective", "omvs", "pmb", "treated"], "article_id"=>813860, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g003", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Altered_AMP_protective_effect_of_OMVs_from_PmB_treated_V_cholerae_/813860", "title"=>"Altered AMP-protective effect of OMVs from PmB treated <i>V. cholerae</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223629"], "description"=>"<p>(A) Detection of Bap1 in OMVs from A1552 grown with PmB (lane 1), OMVs from A1552 grown without OMVs(lane 2), OMVs from Δ<i>ompT</i> mutant grown with PmB (lane 3) and OMVs from Δ<i>toxR</i> mutant grown with PmB (lane 4). (B) Detection of OmpT in OMVs from A1552 grown without PmB (lane 1), OMVs from A1552 grown with PmB (lane 2), whole cell extract of A1552 grown without PmB (lane 3) and whole cell extract of A1552 grown with PmB (lane 4). (C) Detection of OmpT and Bap1 in control-OMVs isolated from Δ<i>ompT</i>/pBR-<i>ompT</i> (lane 1), in PmB-OMVs isolated from Δ<i>ompT</i>/pBR-<i>ompT</i> (lane 2), incontrol-OMVs from Δ<i>ompT</i>/pBR-<i>ompT</i>* (LDV mutant) (lane 3) and PmB-OMVs from Δ<i>ompT</i>/pBR-<i>ompT</i>* (LDV mutant) (lane 4).</p>", "links"=>[], "tags"=>["detection", "ompt", "bap1", "omv", "samples", "extracts"], "article_id"=>813861, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g004", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunoblot_detection_of_OmpT_and_Bap1_in_OMV_samples_and_in_total_cell_extracts_from_whole_cell_bacteria_/813861", "title"=>"Immunoblot detection of OmpT and Bap1 in OMV samples and in total cell extracts from whole cell bacteria.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223631"], "description"=>"<p>(A) Fluorescence microscopy analysis of LL-37 binding to OMVs. Control-OMVs from A1552 grown without AMPs (a, b and c), A1552 OMVs grown with PmB (d, e and f), Δ<i>bap1</i> OMVs grown with PmB (g, h and i) and <i>ΔompT</i> OMVs grown with PmB (j, k, and l) were stained using PKH26 Red Fluorescent dye (a, d, g and j) and LL-37 was detected using anti-LL-37 and FITC-labelled (green) anti-rabbit antibodies (b, e, h and k). Merged images are shown in frames c, f, i and l. (B) Immunoblot quantification of LL-37 binding to control-OMVs from A1552, PmB-OMVs from A1552, PmB-OMVs from Δ<i>bap1</i> and PmB-OMVs from Δ<i>ompT</i>.</p>", "links"=>[], "tags"=>["ll-37", "binding"], "article_id"=>813863, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g005", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_of_LL_37_binding_to_V_cholerae_OMVs_/813863", "title"=>"Detection of LL-37 binding to <i>V. cholerae</i> OMVs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223637"], "description"=>"<p>The following samples were analyzed: a) Control-OMVs from A1552 grown without AMPs, b) OMVs from A1552 grown with PmB, c) OMVs from the <i>Δbap1</i> mutant grown without PmB), d) OMVs from the <i>Δbap1</i> mutant grown with PmB, e) OMVs from the <i>ΔompT</i> mutant grown without PmB, f) OMVs from the <i>ΔompT</i> mutant grown with PmB. Arrows in panel b) indicate examples of gold labeling. Horizontal bars represent 100 nm.</p>", "links"=>[], "tags"=>["labeling", "electron", "microscopic", "ll-37", "binding"], "article_id"=>813869, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g006", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immuno_gold_labeling_and_electron_microscopic_analysis_of_LL_37_binding_to_OMVs_/813869", "title"=>"Immuno-gold labeling and electron microscopic analysis of LL-37 binding to OMVs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223638"], "description"=>"<p>Immunoblot detection of LL-37 after 1 h, 3 h and 6 h of incubation with OMVs from A1552 grown without and with sub-lethal concentration of PmB.</p>", "links"=>[], "tags"=>["pmb", "ll-37", "binding"], "article_id"=>813870, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g007", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_PmB_treatment_on_LL_37_binding_to_V_cholerae_OMVs_/813870", "title"=>"Effect of PmB treatment on LL-37 binding to <i>V. cholerae</i> OMVs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223639"], "description"=>"<p>(A) In the absence of PmB, OMVs cannot protect A1552 against a lethal concentration of LL-37, leading to bacterial death. (B) Growing A1552 with a sub-lethal concentration of PmB can induce the release of OMVs able to bind Bap1 through the LDV domain of OmpT. Bap1 then serves as an adapter protein between LL-37 and the OmpT on the surface of the OMVs. The concentration of free LL-37 was thereby reduced to a sub-lethal concentration, leading to the apparent resistance and survival of <i>V. cholerae</i>. OM: outer membrane, PG: peptioglycan, IM: inner membrane.</p>", "links"=>[], "tags"=>["omvs", "grown", "sub-lethal", "pmb", "cross-resistance", "ll-37"], "article_id"=>813871, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g008", "stats"=>{"downloads"=>1, "page_views"=>75, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Model_for_the_role_of_OMVs_from_V_cholerae_grown_with_sub_lethal_concentration_of_PmB_in_cross_resistance_mechanism_against_LL_37_in_V_cholerae_/813871", "title"=>"Model for the role of OMVs from <i>V. cholerae</i> grown with sub-lethal concentration of PmB in cross-resistance mechanism against LL-37 in <i>V. cholerae</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223640"], "description"=>"<p>Strains and plasmids used in this study.</p>", "links"=>[], "tags"=>["plasmids"], "article_id"=>813872, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.t001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Strains_and_plasmids_used_in_this_study_/813872", "title"=>"Strains and plasmids used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223641"], "description"=>"<p>Primers used in this study.</p>", "links"=>[], "tags"=>[], "article_id"=>813873, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.t002", "stats"=>{"downloads"=>1, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Primers_used_in_this_study_/813873", "title"=>"Primers used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223642", "https://ndownloader.figshare.com/files/1223643"], "description"=>"<div><p>Outer membrane vesicles (OMVs) that are released from Gram-negative pathogenic bacteria can serve as vehicles for the translocation of effectors involved in infectious processes. In this study we have investigated the role of OMVs of the <i>Vibrio cholerae</i> O1 El Tor A1552 strain in resistance to antimicrobial peptides (AMPs). To assess this potential role, we grew <i>V. cholerae</i> with sub-lethal concentrations of Polymyxin B (PmB) or the AMP LL-37 and analyzed the OMVs produced and their effects on AMP resistance. Our results show that growing <i>V. cholerae</i> in the presence of AMPs modifies the protein content of the OMVs. In the presence of PmB, bacteria release OMVs that are larger in size and contain a biofilm-associated extracellular matrix protein (Bap1). We demonstrated that Bap1 binds to the OmpT porin on the OMVs through the LDV domain of OmpT. In addition, OMVs from cultures incubated in presence of PmB also provide better protection for <i>V. cholerae</i> against LL-37 compared to OMVs from <i>V. cholerae</i> cultures grown without AMPs or in presence of LL-37. Using a <i>bap1</i> mutant we showed that cross-resistance between PmB and LL-37 involved the Bap1 protein, whereby Bap1 on OMVs traps LL-37 with no subsequent degradation of the AMP.</p></div>", "links"=>[], "tags"=>["matrix", "bap1", "cross-resistance", "antimicrobial", "peptides"], "article_id"=>813874, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>["https://dx.doi.org/10.1371/journal.ppat.1003620.s001", "https://dx.doi.org/10.1371/journal.ppat.1003620.s002"], "stats"=>{"downloads"=>2, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Role_of_the_Vibrio_cholerae_Matrix_Protein_Bap1_in_Cross_Resistance_to_Antimicrobial_Peptides/813874", "title"=>"Role of the <i>Vibrio cholerae</i> Matrix Protein Bap1 in Cross-Resistance to Antimicrobial Peptides", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-10-03 02:16:50"}
  • {"files"=>["https://ndownloader.figshare.com/files/1223622"], "description"=>"<p>(A) Growth curves of A1552 in normal LB (square), LB supplemented with 12.5 µg/ml of PmB (diamond) and LB supplemented with 12.5 µg/ml of LL-37 (triangles). (B) Electron micrographs of OMVs from A1552 control cultures without AMPs (panel a) or cultures with a sub-lethal concentration of LL-37 (pannel b) or PmB (pannel c). Bars: 50 nm.</p>", "links"=>[], "tags"=>["amp", "omvs"], "article_id"=>813854, "categories"=>["Biological Sciences"], "users"=>["Marylise Duperthuy", "Annika E. Sjöström", "Dharmesh Sabharwal", "Fatemeh Damghani", "Bernt Eric Uhlin", "Sun Nyunt Wai"], "doi"=>"https://dx.doi.org/10.1371/journal.ppat.1003620.g001", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sub_lethal_AMP_treatment_and_OMVs_formation_of_V_cholerae_/813854", "title"=>"Sub-lethal AMP treatment and OMVs formation of <i>V. cholerae</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-03 02:16:50"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
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Relative Metric

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